Combined Short-Term Glucose Starvation and Chemotherapy in 3D Colorectal Cancer Cell Culture Decreases 14-3-3 Family Protein Expression and Phenotypic Response to Therapy

Short-term glucose starvation prior to chemotherapy has the potential to preferentially weaken cancer cells, making them more likely to succumb to treatment, while protecting normal cells. In this study, we used 3D cell cultures of colorectal cancer and assessed the effects of short-term glucose starvation and chemotherapy compared to treatment of either individually. We evaluated both phenotypic changes and protein expression levels. Our findings indicate that the combined treatment results in more significant phenotypic responses, including decreased cell viability and clonogenicity. These phenotypic responses can be explained by the decreased expression of LDHA and 14-3-3 family proteins, which were found only in the combined treatment groups. This study indicates that short-term glucose starvation has the potential to increase the efficacy of current cancer treatment regimes.

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Tartrazine: physical,thermal and biophysical properties of the most widely employed synthetic yellow food-colouring azo dye

Journal of Thermal Analysis and Calorimetry - The food-colouring dye tartrazine is a significant additive and in the same time a biologically active material. Thermal behaviour of trisodium...     

Total Synthesis and Structural Revision of the Antibiotic Tetrapeptide GE81112A

The total synthesis of the naturally occurring antibiotic GE81112A, a densely functionalized tetrapeptide, is reported. Comparison of spectral data with those of the natural product and the lack of biological activity of the synthesized compound led us to revise the published configuration of the 3‐hydroxypipecolic acid moiety. This hypothesis was fully validated by the synthesis of the corresponding epimer.     

Controlling Proton and Electron Transfer Rates to Enhance the Activity of an Oxygen Reduction Electrocatalyst

An electrochemical approach is developed that allows for the control of both proton and electron transfer rates in the O₂ reduction reaction (ORR). A dinuclear Cu ORR catalyst was prepared that can be covalently attached to thiol‐based self‐assembled monolayers (SAMs) on Au electrodes using azide–alkyne click chemistry. Using this architecture, the electron transfer rate to the catalyst is modulated by changing the length of the SAM, and the proton transfer rate to the catalyst is controlled with an appended lipid membrane modified with proton carriers. By tuning the relative rates of proton and electron transfer, the current density of the lipid‐covered catalyst is enhanced without altering its core molecular structure. This electrochemical platform will help identify optimal thermodynamic and kinetic parameters for ORR catalysts and catalysts of other reactions that involve the transfer of both protons and electrons.     

Interrupted Baeyer–Villiger Rearrangement: Building A Stereoelectronic Trap for the Criegee Intermediate

The instability of hydroxy peroxyesters, the elusive Criegee intermediates of the Baeyer–Villiger rearrangement, can be alleviated by selective deactivation of the stereoelectronic effects that promote the 1,2‐alkyl shift. Stable cyclic Criegee intermediates constrained within a five‐membered ring can be prepared by mild reduction of the respective hydroperoxy peroxyesters (β‐hydroperoxy‐β‐peroxylactones) which were formed in high yields in reaction of β‐ketoesters with BF₃?Et₂O/H₂O₂.     

Bis(2,4,6-trifluorophenyl) derivatives of palladium(II)

Summary The organopalladium(II) complexes: Pd(2,4,6-C₆F₃H₂)₂L₂ [L=triphenylphosphine(PPh₃), methyldiphenylphosphine(PPh₂Me), dimethylphenylphosphine-(PPhMe₂) or pyridine(py); L₂=1,2-bis(diphenylphosphino)ethane(dpe), 2,2-bipyridine(bipy), 1, 10-phenanthroline(phen) or ethylenediamine(en)] have been prepared by addition of the appropriate compound to the THF-dioxane solution resulting from the arylation of potassium tetrachloropalladate(II) with (2,4,6-C₆F₃H₂)MgBr. The i.r. data suggest that the py and PPhMe₂ compounds are thecis-isomers, whereas the PPh₃ and PPh₂Me compounds have thetrans configuration.¹H- and¹⁹F-n.m.r. data for the compounds are reported.     

Electrocatalysis of As(III) oxidation by cobalt oxide nanoparticles: measurement and modeling the effect of nanoparticle amount on As(III) oxidation potential

Journal of Solid State Electrochemistry - The performance of electrodes modified with electrochemically generated cobalt oxide nanoparticles for the oxidation of As(III) species was investigated by...     

Formation of metal-anion arrays within layered perovskite hosts. Preparation of a series of new metastable transition-metal oxyhalides, (MCl)LaNb(2)O(7) (M = Cr,Mn, Fe,Co)

A new series of transition-metal oxyhalides (MCl)LaNb(2)O(7) (M = Cr, Mn, Fe, Co) have been prepared by a simple topochemical route. Layered perovskite hosts (ALaNb(2)O(7), A = Li, Na, K or Rb) were reacted with the corresponding anhydrous metal halides under mild reaction conditions (<400 degrees C). The compounds were examined by X-ray powder diffraction; the series appears to be isostructural with (CuCl)LaNb(2)O(7), and the layer spacings, with the exception of M = Co, follow the trend expected from transition-metal cationic radii. Thermal analysis with differential scanning calorimetry (DSC) shows the materials to be metastable where all four compounds decompose exothermically above 690 degrees C.