Voltammetric and spectroscopic studies on the interaction of anti-cancer herbal drug rutin with an anti-tuberculosis agent rifampicin

In this paper, the interaction of rutin (Rt) with rifampicin (RIF) has been investigated using voltammetric and spectroscopic techniques. With the addition of RIF into the Rt solution, both the reduction and oxidation currents of rutin decrease with few changes in the peak potentials and no new peaks appeared. The binding of Rt with RIF forms a kind of supramolecular complex Rt-RIF, which is electrochemically non-active. The experimental data showed that the formation of supramolecular complex is due to the electrostatic attraction of Rt with RIF. The binding reaction resulted in the decrease of the concentration of free Rt in the solution, and the decrease of the cathodic peak current of Rt. The stoichiometry of the Rt-RIF biocomplex was determinated to be 1: 1 by means of voltammetric data. The effect of pH on the binding reaction has been also studied. Based on the peak current values of Rt in the presence and absence of RIF, the binding constants of Rt-RIF at pHs 4, 7 and 9 were calculated as 0.72 × 10⁴ M⁻¹, 1.28 × 10⁴ M⁻¹ and 0.19 × 10⁴ M⁻¹, respectively. The experimental results indicate that there is a strength interaction between Rt and RIF in neutral pH. This binding reaction was supported by UV-Vis. and IR spectroscopy techniques.     

Isotropic matrix elements of the collision integral for the Boltzmann equation

We have proposed an algorithm for constructing matrix elements of the collision integral for the nonlinear Boltzmann equation isotropic in velocities. These matrix elements have been used to start the recurrent procedure for calculating matrix elements of the velocity-nonisotropic collision integral described in our previous publication. In addition, isotropic matrix elements are of independent interest for calculating isotropic relaxation in a number of physical kinetics problems. It has been shown that the coefficients of expansion of isotropic matrix elements in Ω integrals are connected by the recurrent relations that make it possible to construct the procedure of their sequential determination.     

Competitive kinetic study of the amination of organomagnesium and ‐zinc reagents with acetone O‐sulfonyloxime

Kinetic studies are reported for the electrophilic amination of substituted phenylmagnesium bromides and CuCN‐catalyzed phenylzinc chlorides with acetone O‐(2,4,6‐trimethylphenylsulfonyl)oxime in tetrahydrofuran at reflux temperature and at room temperature respectively. Substituent effects were determined by competition experiments. Rate data are analyzed via Hammett relationships to support the proposed mechanisms for the substitution of these organometallics with an electrophilic amino transfer reagent. Copyright © 2005 John Wiley & Sons, Ltd.     

Lifetime of the two-phonon vibrational state in232Th

We report on a measurement of the lifetime of the 1414 keV state in²³²Th by the Doppler-shift recoil-distance method. This 4⁺ state shows all characteristics of a nearly-harmonic two-phonon-vibrational excitation. The result for the B(E2) value obtained from the lifetime,=3.2 ± 0.7 ps, is in agreement with an earlier estimate of the collectivity from Coulomb-excitation yields and supports the interpretation of the 1414 keV state as a two-phonon-vibrational excitation.We would like to thank the accelerator crew of the Max-Planck-Institut für Kernphysik for the excellent running conditions they maintain. We are also indebted to Dr. H.J. Maier, University Munich for supplying us with the²³²Th targets. This work was supported by the Bundesminister für Forschung und Technologie under contract numbers 06BN601 and 06HD5251.     

The interaction of cysteine with chromium(VI) ions under UV irradiation

In 0.1 M phosphate buffer (pH 7.2), the interaction of chromium(VI) with cysteine in the presence and absence of UV irradiation was studied by cyclic voltammetry and electronic spectroscopy techniques. The reduction of Cr(VI) by cysteine takes place through the formation of Cr(VI)-thioester intermediate. On the cyclic voltammograms of cysteine and Cr(VI) mixture, the peaks at -0.315 and -0.800 V were observed, and these peaks are corresponding to the reduction of Cr(VI)-thioester and thiyl radical, respectively. In the cysteine solution exposed to UV irradiation, the formation of free cystine was observed at -0.792 V. In the cysteine and Cr(VI) mixture exposed to UV irradiation, the peak current of thiyl radical increases while the peak current of Cr(VI)-thioester reaches a maximum at 15 min and then decreases by increasing UV irradiation time. The formation of the thioester in the reaction between Cr(VI) and cysteine in aqueous media has been studied by monitoring the decrease of Cr(VI) at 370 nm. It was observed that the reaction is catalyzed by the UV irradiation of the Cr(VI) and cysteine mixture.     

High-energy gamma-rays accompanying the spontaneous fission of252Cf

Theγ-ray spectrum of²⁵²Cf(sf) was measured in the Darmstadt-Heidelberg Crystal Ball spectrometer, with a double ionization chamber mounted inside to detect the fission fragments. The measurement was aimed at a better understanding of an unusual component found in the high-energy region between 3 and 8 MeV, with fragment mass splits near symmetry. This component was proved to be predominantly emitted by the heavier fragment, to reach its highest intensity at a fragment mass split of 132:120, and to have an almost isotropic angular distribution. Calculations with the statistical code CASCADE could reproduce the main features.     

Coincidence Doppler shift lifetime measurements in 73,74Se and 74Br using the EUROBALL Cluster cube array

Subpicosecond lifetimes of high spin states in the rotational nuclei ⁷³Se, ⁷⁴Se and ⁷⁴Br have been measured using the ⁵⁸Ni +¹⁹F compound reaction and the Doppler Shift Attenuation method. Six EUROBALL Cluster detectors arranged in cube geometry allowed us to select the relevant transitions in γγ coincidence mode. The high counting statistics achieved in this setup also facilitated the determination of average sidefeeding times, which were found to agree rather well with the results of Monte-Carlo calculations of the particle and γ-ray evaporation process. The deduced quadrupole strengths and deformations are compared with the results of previous measurements and the predictions of Cranked Shell Model calculations. In ⁷⁴Br, a large and constant prolate deformation of β₂= 0.37(1) was found for the presumed 4⁺ and 3⁻ two-quasiparticle bands. Received: 2 July 1999     

Betr?ge zur Kenntnis der Ligninbestimmung nach der Schwefels?uremethod

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