Second-class currents in 0−→0+ and unique nuclear β transitions

The induced pseudotensor contribution to 0^? → 0⁺ and uniqueβ transitions has been analyzed starting from two possible forms of the nonrelativistic Hamiltonian. The role of mesonic corrections and mesonic second-class currents has been discussed     

Development of a differential pulse voltammetric method for the determination of Silymarin/Vitamin E acetate mixture in pharmaceuticals

Differential pulse voltammetric method was developed for determination of Silymarin (SMR)/Vitamin E acetate (VEA) mixture in pharmaceuticals. SMR and VE gave well-resolved diffusion-controlled anodic peaks at +756 and +444mV, respectively (versus Ag/AgCl) in Britton-Robinson buffer at pH 2.8. The solution conditions and instrumental parameters were optimized for their quantitative determination. The linear response was obtained in the range 0.1-4.0mgL(-1) with a detection limit of 0.03mgL(-1) for SMR and 0.05-4.0mgL(-1) with a detection limit of 0.01mgL(-1)for VEA.     

Real-time monitoring of protein complexes reveals their quaternary organization and dynamics

The dynamics of protein complexes are crucial for their function yet are challenging to study. Here, we present a nanoelectrospray (nESI) mass spectrometry (MS) approach capable of simultaneously providing structural and dynamical information for protein complexes. We investigate the properties of two small heat shock proteins (sHSPs) and find that these proteins exist as dodecamers composed of dimeric building blocks. Moreover, we show that these proteins exchange dimers on the timescale of minutes, with the rate of exchange being strongly temperature dependent. Because these proteins are expressed in the same cellular compartment, we anticipate that this dynamical behavior is crucial to their function in vivo. Furthermore, we propose that the approach used here is applicable to a range of nonequilibrium systems and is capable of providing both structural and dynamical information necessary for functional genomics.     

Eco-friendly micellar HPTLC technique for the simultaneous analysis of co-formulated antibiotic cefoperazone and sulbactam in pure form and vial pharmaceutical formulation

JPC – Journal of Planar Chromatography – Modern TLC - A rapid, sensitive, and eco-friendly micellar high-performance thin-layer chromatography (HPTLC) technique for the simultaneous...     

A Site-Selective Doping Strategy of Carbon Anodes with Remarkable K-Ion Storage Capacity

The limited potassium-ion intercalation capacity of graphite hampers development of potassium-ion batteries (PIB). Edge-nitrogen doping is an effective approach to enhance K-ion storage in carbonaceous materials. One shortcoming is the lack of precise control over producing the edge-nitrogen configuration. Here, a molecular-scale copolymer pyrolysis strategy is used to precisely control edge-nitrogen doping in carbonaceous materials. This process results in defect-rich, edge-nitrogen doped carbons (ENDC) with a high nitrogen-doping level (up to 10.5 at %) and a high edge-nitrogen ratio (87.6 %). The optimized ENDC exhibits a high reversible capacity of 423 mAh g⁻¹, a high initial Coulombic efficiency of 65 %, superior rate capability, and long cycle life (93.8 % retention after three months). This strategy can be extended to design other edge-heteroatom-rich carbons through pyrolysis of copolymers for efficient storage of various mobile ions.     

The hypoglycemic and antioxidant activities of garden cress (Lepidium sativum L.) seed on alloxan-induced diabetic male rats

The hypoglycemic and antioxidants activities of the methanol extract of Lepidium sativum seeds was tested in alloxan-induced diabetic male rats. Thirty male albino rats weighing 190–200 g were divided into five groups as follows: negative control, positive control and three diabetic groups treated with three concentrations of L. sativum methanol extract for four weeks. Induction of hyperglycemia in the positive control group increased blood glucose, glycated hemoglobin A1c, immunoglobulins, liver enzyme, lipid peroxide and kidney function, total cholesterol, low-density lipoproteins, very low-density lipoproteins and decreased antioxidants and high-density lipoproteins compared with the negative control. Furthermore, pancreas tissues showed pathological changes compared with the negative control. Treating the diabetic rats with L. sativum methanol extract decreased blood sugar and restored all biochemical and histological changes to the normal. It could be concluded that L. sativum methanol extract succeeded in controlling diabetes, increasing antioxidants and ameliorating lipid profile.     

Preconcentration of milk proteins using octadecylated monolithic silica microchip

Sample preparation is a bottleneck in systems for chemical analysis and it is a required step in order to remove interference and preconcentrate the target analytes. Much research in recent years has focused on porous monolithic materials since they are highly permeable to liquid flow and show high mass transfer compared with common packed beds. This study has focused on the use of a glass microchip containing an inorganic silica-based monolithic material modified with octadecyl groups for preconcentration of milk proteins from skimmed cows’ milk that vary in molecular weight, hydrophobicity, and abundance. Comparison between the fabricated device and a commercial cartridge for the preconcentration of proteins in skimmed cows’ milk showed the ability of the device to successfully enrich protein mixtures from the sample. The three replicate experiments showed that the RSD of the mass to charge ratio of milk proteins ranged from 0.01 to 0.46%. In addition, it was found that there were no significant differences between the observed and reported masses of the milk proteins and the relative percentage error of the molecular masses ranged between 0.03 and 0.90%. The fact that the small amounts of sample required and short sample preparation time suggest that this new microfluidic device may be a viable alternative to existing procedures for protein extraction from real samples.     

Facile Route for the Synthesis of Pyridazine Derivatives: Unexpected Pathway to Benzothiazole,Benzimidazole, and Triazole Derivatives

4‐Amino‐5‐arylmethylidene‐3‐phenyl‐pyridazin‐6‐ones 7 have been synthesized and reacted with selected nucleophile reagents such as phenyl hydrazine, semicarbazide, semithiocarbazide, cyanoacetohydrazide, 2‐aminothiophenol, and 2‐phenylenediamine in ethanol triethyl‐amine solution. An unexpected 1‐phenyl‐3‐arylaziridene 10, 3‐aryl‐5‐oxo(thio)‐1,2,4‐triazole 21, 4‐amino‐3‐aryl‐6‐hydroxy‐pridazine 27, 2‐arylbenzothiazole 30a–c, and 2‐arylbenzimidazole 30d–f have been obtained, respectively. Also, 2‐aminothiophenol and 2‐phenylenediamine were reacted with N‐phenylmethylidene‐2‐cyanoacetohydrazide 2, affording the new 1,4‐benzodiazepine derivatives 35.     

Simultaneous quantitative determination of insulin aspart and insulin degludec in human plasma using simple shoot LC method

Combining short-acting and long-acting insulin analogs was a real challenge that was overcome by NovoNordisk through the co-formulation of insulin aspart and insulin degludec in single-dosage form. The proposed study provides a simple, short, and reliable HPLC method with diode array detection that is developed and validated for the simultaneous determination of insulin aspart and insulin degludec in human plasma. The proposed method achieved good separation between the two analytes utilizing a C8 column at 35°C in a very short run time (6 min), with a simple, low-cost, and reliable extraction method through precipitation of plasma protein. Gradient elution was applied using a mobile phase consisting of 0.1 M sodium sulfate (pH 3.4) and acetonitrile. The method was validated according to EMA Guideline on Bioanalytical Validation. The proposed method had a linear range from 3.0 to 300 μg/mL for insulin aspart and from 3.5 to 300 μg/mL for insulin degludec. The intra- and inter-day precision of insulin aspart were 0.36–3.33% and 1.59–8.84%, respectively, and accuracy was between 10.06 and 3.09% The intra- and inter-day precision of insulin degludec were 0.29–1.93% and 0.89–5.14%, respectively, and accuracy was between −5.29 and 3.91%.