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31.
The purpose of this study was to investigate the effect of three vitrification procedures [conventional straw (CS), open pulled straw (OPS) and closed pulled straw (CPS)] on the development of two-cell mouse embryos. Two-cell mouse embryos were randomly divided into vitrified and non-vitrified control groups. Embryos in the vitrified group were cryopreserved within a combination of 5.5 M ethylene glycol and 1M sucrose as cryoprotectants, loaded within three different straws (CS, OPS and CPS) and warmed in stepwise sucrose solutions. The survived embryos from each procedure were cultured in human tubal fluid (HTF). The non-vitrified control embryos were also cultured in the same manner. The rates of the development in all the groups were daily determined and statistically compared. On day 4 of the cultivation period, several expanded blastocysts from each group were randomly selected and stained either with propidium iodide (PI) and bisbenzimide or with terminal transferase- mediate DNA end labeling (TUNEL) Technique. The mean number of the inner cell mass (ICM), trophoectoderm (TE), necrotic and apoptotic cells were counted and statistically compared. The survival rate of embryos in CPS was significantly higher than that in OPS and CS. The rate of hatched blastocysts did not differ in the three vitrification procedures, but in comparison with that of the control, CS and OPS showed a significant reduction. The mean number of ICM and TE decreased in CS and OPS, whereas in CPS it was almost identical to that of the control. The incidence of apoptosis and necrosis appeared to be almost similar in all the groups. In conclusion, CPS seems to be an effective, easy and rapid method for the cryopreservation of two-cell mouse embryos. 相似文献
32.
T.?HayatEmail author K.?Hutter S.?Nadeem S.?Asghar 《Zeitschrift für Angewandte Mathematik und Physik (ZAMP)》2004,55(4):626-641
The purpose of this work is to investigate the hydromagnetic oscillatory flow of a fluid
bounded by a porous plate, when the entire system rotates about an axis normal to the plate. The
fluid is assumed to be non-Newtonian (second grade), incompressible and electrically conducting.
The magnetic field is applied transversely to the direction of the flow. Such a flow model has
great significance not only of its theoretical interest, but also for applications to geophysics
and engineering. The resulting initial value problem has been solved analytically for steady and
unsteady cases. The analysis of the obtained results showed that the flow field is appreciably
influenced by the material parameter of the second grade fluid, the applied magnetic field, the
imposed frequency, rotation and suction and blowing parameters. It is observed in a second grade
fluid that a steady asymptotic hydromagnetic solution exists for blowing and resonance which is
different from the hydrodynamic situation. 相似文献
33.
Mehdi Ramezani Mehdi Dehghan Mohsen Razzaghi 《Numerical Methods for Partial Differential Equations》2008,24(1):1-8
In this work the combined finite difference and spectral methods have been proposed for the numerical solution of the one‐dimensional wave equation with an integral condition. The time variable is approximated using a finite difference scheme. But the spectral method is employed for discretizing the space variable. The main idea behind this approach is that we can get high‐order results. The new method is used for two test problems and the numerical results are obtained to support our theoretical expectations. © 2007 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq, 2007 相似文献
34.
35.
Summary Love wave travelling in a layer of uniform thickness overlying a half-space is assumed to be incident on two parallel but
staggered perfectly weak half-planes lying in the upper layer. The diffracted field is calculated using the modified Wiener-Hopf
technique and contour integration method. The diffracted waves satisfy the dispersion relations appropriate to different regions
formed by the perfectly weak half-planes.
To the memory of M. H. Kazi 相似文献
36.
Summary The problem of calculating the field within imperfectly conducting parallel-plates is considered. The solution in closed form is obtained using the Wiener-Hopf technique. The comparison has been made with that of perfectly conducting parallel plates waveguide.
Zusammenfassung Das Problem der Feldberechnung in unvollständig leitenden parallelen Platten wird betrachtet. Die Lösung in geschlossener Form wird mittels der Wiener-Hopf Technik gefunden, sowie ein Vergleich mit der Lösung für vollständig leitende Parallelplatten-Wellenleiter durchgeführt.相似文献
37.
38.
Exact analytical solutions for a class of unsteady unidirectional flows of an incompressible second-order fluid are constructed. The flows are generated impulsively from rest by motion of a plate or two plates or by sudden application of a pressure gradient. Expressions for velocity, flux and skin friction are obtained for both large and small times. It is found that large and small times solutions are dependent on the coefficient of viscoelasticity. The solutions corresponding to Newtonian fluids can be easily obtained from those for fluids of second order by letting the viscoelastic parameter to be zero. 相似文献
39.
A simple, fast, reproducible (2.5% RSD at 3.0 μg/L), and sensitive method is described for quantifying As(III) (0.3 μg/L detection limit, 0.5–440 μg/L dynamic range). Anodic stripping voltammetry (ASV) is performed after accumulating arsenic at a mercury film electrode at ?0.350 V vs. Ag/AgCl (saturated KCl) for 20 s in 0.2 M HCl containing 8 μM ammonium 2‐amino‐1‐cyclopentene‐1‐dithiocarboxylate (AACD), without oxygen removal. This is the first report of using AACD in ASV and in electrochemical quantification of As(III). Total arsenic is determined after sodium‐sulfite‐reduction of As(V) to As(III). Interferences are minimal. Method validation involved water and metal alloy samples. 相似文献
40.
Ali Asghar Rastegari Abdol-Khalegh Bordbar Vajihe Mehnati-Najafabadi 《Cellulose (London, England)》2010,17(6):1213-1225
The present study evaluates the binding of cetylpyridinium chloride (CPC) with cellulase in various experimental conditions
using potentiometric, fluorescence spectroscopy and turbidimetric techniques. The analysis of binding curves revealed the
existence of two sets of binding sets for CPC. The binding parameters were estimated and interpreted in terms of structural
viewpoints of cellulase. The observation of turbidity suggests that CPC molecules individually nucleate around cellulase/CMC
complex to form micelle-like structures. Fluorescence spectroscopy analysis of cellulase/CMC-surfactant system showed that
these complexes could be compact to elucidate the mechanism of binding cellulase/CMC complex to CPC. The differential response
of the enzyme/CMC to surfactant, indicates that the interaction on the complex surface is strongly ionic and hydrophobic(cooperative)
in nature. A sequencing analysis was also conducted on β-1, 4-endoglucanase from A. niger (EglA) and others from family 12 in order to examine the nature of interaction involved in binding process and structure
of carbohydrate-protein complexes. The results suggest that the conserved residues are located in a more hydrophobic microenvironment
and apolar area energy is more than polar within enzyme structure. 相似文献