葵花籽粕蛋白质、绿原酸分步提取工艺参数优化

在单因素试验的基础上,选取NaCl浓度、提取温度、料液比为自变量,以蛋白质提取率为响应值;选取乙醇体积分数、提取时间、料液比为自变量,以绿原酸的提取率为响应值,利用响应面法对葵花籽粕蛋白质、绿原酸的提取工艺进行优化,得到回归方程的预测模型。结果表明,最佳提取工艺条件为:蛋白质NaCl浓度1.51mol/L、液料比1∶12.88(g/mL)、提取温度60.27℃;绿原酸乙醇体积分数56.07%、液料比1∶16.03(g/mL)、提取时间47.06min.在此条件下,葵花籽粕蛋白质和绿原酸的提取率分别为36.64%和2.73%.     

高效液相色谱手性流动相添加剂法拆分佐匹克隆对映体

以β-环糊精作为手性流动相添加剂,研究了佐匹克隆对映体在反相高效液相色谱系统中的拆分情况。探讨了β-环糊精浓度、流动相中甲醇含量、流动相pH值、流动相流速及温度等因素对佐匹克隆对映体拆分的影响。通过采用单一变量法对实验条件进行考察,最终得到了佐匹克隆对映体手性拆分的最佳色谱条件。结果表明:当采用Inertsil ODS-SP(5μm,4.6 mm×250 mm)色谱柱,甲醇为有机相,流动相为20mmol/L磷酸二氢钾缓冲液(含15 mmol/Lβ-环糊精)-甲醇(85∶15),流速为0.8 mL/min,pH值为5.5,温度为35℃,紫外检测波长为305 nm,进样量为20μL时,佐匹克隆对映体得到良好分离,其对映体的保留时间分别为67.42 min和74.48 min,分离度(R)为1.62。该方法操作简单,灵敏度高,拆分效果理想,且比手性固定相法成本低,从而为佐匹克隆对映体的进一步深入研究提供了一种新方法。     

APPELL方程和TZNOFF方程在一般非完整系统上的推广

本文给出一个适用于任何阶非完整系统的变分原理。Gauss原理可作为推论由之导出。由此原理出发,将Appell方程和Tzènoff方程推广到一般非完整系统,以一个二阶非完整系统为例,说明所得方程的应用。     

Self—assembly and photoelectric properties of cerium compleses with 3,4,9,10—perylenetetracarboxylic acid on nanocrystalline TiO2 films

Self-assembled(SA)films (PMP,M=Ce^3 or Ce^4 )of 3,4,9,10-perylenetetracarboxylic acid(PTA)on nanocrystalline TiO2 films with Ce^3 or Ce^4 as a bridge were fabricated and characterized with UV-Vis,IR,and XPS synchrotron radiation photoelectron spectroscopy (SRPES) which gave the HOMO energy levels for the SA films.It was shown that thin-layer sndwich-type solar cells based on these SA films possess good properties for photoelectric conversion.While PTA-loaded TiO2 electrode(P) generated 26.9?f incident monochromatic phototo-to-electron conversion efficiency (IPCE),PMP-sensitized TiO2 electrodes yielded 55.8?and 39.1?for Ce^4 and Ce^3 respectively.PMP films can be considered as a kind of complexes whose HOMO energy levels were proved to eb higher than that of film P,which is one of the major reasons for the increase in IPCE form film P to film PMP.     

Methods of calibration to optical trapping force upon non-spherical cells

The dynamical equation of a trapping cell is solved to find calibration methods for the trapping force, and two methods are compared by synthetic experiment data. Results indicate that: Boltzmann distribution method (BDM) is available for the force calibration of non-spherical or anisotropic cells in arbitrary trap potential; the mean square displacement method (MSDM) is available only for a symmetric harmonic optical trap. The spatial resolution requirement of the calibration system is about a nanometer. The results agree with the reported experiments.     

古紫质AR4重组的磷脂脂质体中的定向自组装

This paper reports, for the first time, that Archaerhodopsin-4 (AR4) could be reconstituted into phospholipid liposomes by self-assembly. AR4 is a new membrane protein isolated from halobacteria H.sp. xz515 in a salt lake of Tibet, China. This is a bacteriorhodopsin (bR) like protein, function as a light-driven proton pump. Experimental measurements verified that similar to bR, AR not only remains its biological activity in pmteoliposome, but also keeps a preferred orientation in self-assembly.     

Self-assembly and photoelectric properties of cerium complexes with 3,4,9,10-perylenetetracarboxylic acid on nanocrystalline TiO_2 films

Self-assembled (SA) films (PMP, M = Ce3+ or Ce4+) of 3,4,9,10-perylenetetracarboxylic acid (PTA) on nanocrystalline TiO2 films with Ce3+ or Ce4+ as a bridge were fabricated and characterized with UV-Vis, IR, and XPS synchrotron radiation photoelectron spectroscopy (SRPES) which gave the HOMO energy levels for the SA films. It was shown that thin-layer sandwich-type solar cells based on these SA films possess good properties for photoelectric conversion. While PTA-loaded TiO2 electrode (P) generated 26.9% of incident monochromatic photon-to-electron conversion efficiency (IPCE), PMP-sensitized Ti02 electrodes yielded 55.8% and 39.1% for Ce4+ and Ce3+ respectively. PMP films can be considered as a kind of complexes whose HOMO energy levels were proved to be higher than that of film P, which is one of the major reasons for the increase in IPCE from film P to film PMP.     

利用LMBE生长BaTiO3薄膜及其原位光电子能谱测量

利用激光分子束外延系统, 在STO:Nb衬底制备了高质量的BaTiO3单晶薄膜, 并在 北京同步辐射站4B9B对其进行了原位的光电子能谱的测量. XPS测量表明, 所生长的薄膜没有Ti离子变价. 实验中测得的价带谱与用ab initio方法计算的结果一致.     

单壁和多壁碳纳米管的硝酸氧化对比研究

利用同步辐射X射线近边吸收谱（XANES）分别对硝酸氧化处理后的单壁碳纳米管和多壁碳纳米管进行了系统的对比研究。实验结果表明,单壁碳纳米管容易遭到硝酸的破坏,生成大量的碳碎片,氧化所产生的氧化基团大部分形成于碳碎片上,这些吸附在碳纳米管上的碳碎片可以通过氢氧化钠溶液的清洗、过滤去除。相比之下,结构稳定性更高的多壁碳纳米管在硝酸处理过程中则显得比较稳定,大量的氧化基团形成于碳管管壁上。这种不同结构碳纳米管的不同氧化结果可能会对碳纳米管的后续修饰和应用产生重要的影响。