RESEARCH OF STEPPING PRESSURE AND CONTROL OF NEAR FIELD ROOF OF TASHAN COAL MINE BASED ON NUMERICAL SIMULATION1)

煤层坚硬顶板采用水力压裂技术进行定向弱化可有效控制来压步距,降低事故风险。基于黏聚力模型,利用有限元二次开发建立了压裂前后煤层开采数值计算模型,模拟分析了顶板垮落规律及影响因素,优化裂缝间距设计。结果表明：来压跨距与顶板厚度、强度呈正相关性,与埋深呈负相关性。预制裂缝在拉应力集中区可促进起裂扩展,应全面勘测各岩层位地质参数,利用计算出的应力集中区位置设计裂缝间距,实现经济有效地控制来压步距。     

Analysis of rabbit tear proteins by high-pressure liquid chromatography/electrospray ionization mass spectrometry

The aim of this study was to develop a fast and reliable analytical procedure for the display of the protein components of tears that can be used to differentiate the status of the ocular surface. Using this new procedure, we analyzed the tear protein components following a corneal wound in the rabbit. Calibrated 10-microL glass, fire-polished capillary micropipettes were used to collect tears from New Zealand White rabbits prior to and daily for 9 days following a unilateral 6-mm diameter centrally placed anterior keratectomy. Tear proteins were eluted by a reversed-phase high-performance liquid chromatography (RP-HPLC) column and the tear protein profile was monitored by electrospray ionization (ESI) mass spectrometry positive total ion current (TIC) chromatography. Tear proteins were reliably separated into 17 peaks, each of which contained one or a number of protein components. The molecular weight of each protein component was determined by on-line ESI. Major tear protein components, lactoferrin, lysozyme (minimally detectable in rabbit tears), albumin, lipocalin, lipophilin and beta2-microglobulin, were tentatively identified by this method. Based on the mass spectrometric data, beta2-microglobulin was found to be glycosylated with N-acetylhexosamine. ESI-positive TIC chromatograms and mass spectra revealed comparative differences in the tear protein spectra after corneal wounding. One day after wounding, rabbit lysozyme with a molecular weight of 14,717 Da was found to be 8-fold higher in the tears of wounded eyes when compared with tears from unwounded eyes. It dropped back to normal 3 days after wounding. The expression of an unidentified tear protein with the molecular weight of 16,060 Da was also elevated after corneal wounding and returned to normal level by day 5. In this study, LC/ESI-MS was developed as a fast, reproducible and simple method for the identification and analysis of many of the protein components of the tears. Importantly, this technique also allows quantification of each component resolved in the chromatogram. This method is very suitable for mapping peptides and proteins (<80 kDa) in tears.     

Highly specific revelation of rat serum glycopeptidome by boronic acid-functionalized mesoporous silica

Although the specific profiling of endogenous glycopeptides in serum is highly inclined towards the discovery of disease biomarkers, studies on the endogenous glycopeptides (glycopeptidome) have never been conducted because of several factors. These factors include the high dynamic range of serum proteins, the inadequacy of traditional sample preparation techniques in proteomics for low-molecular-weight (LMW) proteins, and the relatively low abundances of glycopeptides. Boronic acid-functionalized mesoporous silica was synthesized in this study to overcome the limitations of the state-of-the-art methods for glycopeptidome research. The boronic acid-functionalized mesoporous silica exhibited excellent selectivity by analyzing glycopeptides in the mixture of glycopeptides/non-glycopeptides at molar ratio of 1:100, extreme sensitivity (the limit of detection was at the fmol level), good binding capacity (40 mg g⁻¹), as well as the high post-enrichment recovery of glycopeptides (up to 88.10%). The as-prepared material possessing both glycopeptide-suitable pore size and glycopeptide-specific selectivity has shown special capability for enriching the endogenous glycopeptides. Fifteen unique glycosylation sites mapped to 15 different endogenous glycopeptides were identified in rat serum. The established protocol revealed for the first time the rat serum glycopeptidome.     

二维层状GaTe的晶体结构研究

采用布里奇曼法生长出了化合物半导体GaTe,并对其晶体结构进行研究.利用X射线衍射仪对GaTe粉末和块体试样分别进行测试,确定了其晶体结构.采用透射电子显微镜对二维薄膜GaTe及二维纳米层GaTe进行观测,并结合晶体定向仪对GaTe解理面进行了定向分析.结果表明,二维薄膜GaTe及二维纳米层GaTe分别对应单斜和六方两种不同的晶体结构,并对应不同的解理面.     

用于聚乙烯表面改性的紫外光接枝技术

从STS角度出发,结合新课标高中化学高分子知识,介绍一种聚乙烯表面改性技术--紫外光接枝技术,供一线高中化学教师用于拓展高中生的高分子化学知识。     

Chiral susceptibility and chiral phase transition in Nambu–Jona-Lasinio model

We give a general relation between the chiral susceptibility and the thermodynamical potential and a relation between the chiral susceptibility and the condition for furcations to appear in the Wigner solution(s) in the Nambu–Jona-Lasinio (NJL) model. We find that the chiral susceptibility is a quantity able to represent the appearance of furcation in the solution(s) of the gap equation and the concavo–convexity of the thermodynamical potential in the NJL model. It indicates that the chiral susceptibility can identify the stability of the states and the chiral phase transition in NJL model. We propose that analyzing the chiral susceptibility may play an important role in studying the chiral phase transition in approaches superior to the NJL model.