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101.
A system for expression and in situ display of recombinant proteins on a microbead surface is described. Biotinylated PCR products were immobilized on microbead surfaces, which were then embedded in a gel matrix and supplied with translation machinery and substrates. Upon the incubation of the gel matrix, target proteins encoded on the bead-immobilized DNA were expressed and captured on the same bead, thus allowing bead-mediated linkage of DNA and encoded proteins. The new method combines the simplicity and convenience of solid-phase separation of genetic information with the benefits of cell-free protein synthesis, such as instant translation of genetic information, unrestricted substrate accessibility and flexible assay configuration design. 相似文献
102.
Tiwari R Toppino A Agarwal HK Huo T Byun Y Gallucci J Hasabelnaby S Khalil A Goudah A Baiocchi RA Darby MV Barth RF Tjarks W 《Inorganic chemistry》2012,51(1):629-639
The synthesis and initial biological evaluation of 3-carboranylthymidine analogues (3CTAs) that are (radio)halogenated at the closo-carborane cluster are described. Radiohalogenated 3CTAs have the potential to be used in the radiotherapy and imaging of cancer because they may be selectively entrapped in tumor cells through monophosphorylation by human thymidine kinase 1 (hTK1). Two strategies for the synthesis of a (127)I-labeled form of a specific 3CTA, previously designated as N5, are described: (1) direct iodination of N5 with iodine monochloride and aluminum chloride to obtain N5-(127)I and (2) initial monoiodination of o-carborane to 9-iodo-o-carborane followed by its functionalization to N5-(127)I. The former strategy produced N5-(127)I in low yields along with di-, tri-, and tetraiodinated N5 as well as decomposition products, whereas the latter method produced only N5-(127)I in high yields. N5-(127)I was subjected to nucleophilic halogen- and isotope-exchange reactions using Na(79/81)Br and Na(125)I, respectively, in the presence of Herrmann's catalyst to obtain N5-(79/81)Br and N5-(125)I, respectively. Two intermediate products formed using the second strategy, 1-(tert-butyldimethylsilyl)-9-iodo-o-carborane and 1-(tert-butyldimethylsilyl)-12-iodo-o-carborane, were subjected to X-ray diffraction studies to confirm that substitution at a single carbon atom of 9-iodo-o-carborane resulted in the formation of two structural isomers. To the best of our knowledge, this is the first report of halogen- and isotope-exchange reactions of B-halocarboranes that have been conjugated to a complex biomolecule. Human TK1 phosphorylation rates of N5, N5-(127)I, and N5-(79/81)Br ranged from 38.0% to 29.6% relative to that of thymidine, the endogenous hTK1 substrate. The in vitro uptake of N5, N5-(127)I, and N5-(79/81)Br in L929 TK1(+) cells was 2.0, 1.8, and 1.4 times greater than that in L929 TK1(-) cells. 相似文献
103.
Cheng‐Yao Lin Jerry Becker Mi‐Ran Byun Der‐Ching Yang Tsai‐Wei Huang 《School science and mathematics》2013,113(1):41-51
This study examined (a) the differences in preservice teachers’ procedural knowledge in four areas of fraction operations in Taiwan and the United States, (b) the differences in preservice teachers’ conceptual knowledge in four areas of fraction operations in Taiwan and the United States, and (c) correlation in preservice teachers’ conceptual knowledge and procedural knowledge of fractions in Taiwan and the United States. Participants were preservice teachers (N = 49) in a teacher education program in the United States and comparable Chinese preservice teachers (N = 47). Results indicated that Chinese preservice teachers performed better in procedural knowledge on fraction operations than American preservice teachers. No significant differences were found for conceptual knowledge on fraction division. Further, the correlation in this study showed that for Chinese and American preservice teachers, the relationship between conceptual and procedural knowledge of fraction operations was weak. 相似文献
104.
Quasiparticle self-consistent many-body perturbation theory (MBPT) methods that update both eigenvalues and eigenvectors can calculate the excited-state properties of molecular systems without depending on the choice of starting points. However, those methods are computationally intensive even on modern multi-core central processing units (CPUs) and thus typically limited to small systems. Many-core accelerators such as graphics processing units (GPUs) may be able to boost the performance of those methods without losing accuracy, making starting-point-independent MBPT methods applicable to large systems. Here, we GPU accelerate MOLGW, a Gaussian-based MBPT code for molecules, with open accelerators (OpenACC) and achieve speedups of up to over 32 open multi-processing (OpenMP) CPU threads. 相似文献
105.
We studied the thermal stability of HfO2 on an InP structure when an Al2O3 interface passivation layer (PL) was introduced. In contrast to the thick (~4 nm) Al2O3‐PL, an almost complete disappearance of the thin (~1 nm) Al2O3‐PL was observed after a post‐deposition anneal at 600 °C. Based on various chemical and electrical analyses, this was attributed to the intermixing of the thin Al2O3‐PL with HfO2, which might have been accompanied by the out‐diffusion of a substantial amount of substrate elements. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
106.
107.
Consider the Lie group SO0(n, 1) with the left-invariant metric coming from the Killing-Cartan form. The maximal compact subgroup SO(n) of the isometry group acts from the left and right. This paper studies the geometry of the quotient space of the homogeneous submersion SO0(n, 1) → SO(n)\SO0(n, 1). It is a cohomogeneity one manifold, which can be expressed as a warped product. Its group of isometries, geodesics, and sectional curvatures are calculated. 相似文献
108.
MBHA (4-methylbenzhydrylamine) resin has been extensively used as a solid support for the synthesis of peptide amides. Herein, we prepared the core-shell-type MBHA resin by benzotriazole-catalyzed amidoalkylation and partial hydrolysis. The core-shell structure of the MBHA resin was confirmed by two-photon microscopy and its synthetic performance in solid-phase peptide synthesis (SPPS) was evaluated. 相似文献
109.
Wongi Jang Richard Taylor IV Pascal N. Eyimegwu Prof. Hongsik Byun Prof. Jun-Hyun Kim 《Chemphyschem》2019,20(1):70-77
Composite materials consisting of nanoscale gold particles and protective polymer shells were designed and tested as catalysts in various chemical reactions. Initially, the systematic incorporation of multiple gold nanoparticles into a poly(N-isopropylacrylamide) particle was achieved by an in situ method under light irradiation. The degree of gold nanoparticle loading, along with the structural and morphological properties, was examined as a function of the amount of initial gold ions and reducing agent. As these gold nanoparticles were physically-embedded within the polymer particle in the absence of strong interfacial interactions between the gold nanoparticles and polymer matrix, the readily-accessible surface of the gold nanoparticles with a highly increased stability allowed for their use as recyclable catalysts in oxidation, reduction, and coupling reactions. Overall, the ability to integrate catalytically-active metal nanoparticles within polymer particles in situ allows for designing novel composite materials for multi-purpose catalytic systems. 相似文献
110.
Jeong Moon Jihyun Byun Hongki Kim Jinyoung Jeong Eun‐Kyung Lim Juyeon Jung Soojeong Cho Woo Kyung Cho Taejoon Kang 《Macromolecular bioscience》2019,19(6)
For the construction of high‐performance biosensor, it is important to interface bioreceptors with the sensor surface densely and in the optimal orientation. Herein, a simple surface modification method that can optimally immobilize antibodies onto various kinds of surfaces is reported. For the surface modification, a mixture of polydopamine (PDA) and protein G was employed. PDA is a representative mussel‐inspired polymer, and protein G is an immunoglobulin‐binding protein that enables an antibody to have an optimal orientation. The surface characteristics of PDA/Protein G mixture‐coated substrates are analyzed and the PDA/protein G ratio is optimized to maximize the antibody binding efficiency. Moreover, the antibody‐immobilized substrates are applied to the detection of influenza viruses with the naked eye, providing a detection limit of 2.9 × 103 pfu mL‐1. Importantly, the several substrates (glass, SiO2, Si, Al2O3, polyethylene terephthalate, polyethylene, polypropylene, and paper) can be modified by simple incubation with the mixture of PDA/protein G, and then the anti‐influenza A H1N1 antibodies can be immobilized on the substrates successfully. Regardless of the substrate, the influenza viruses are detectable after the sandwich immunoreaction and silver enhancement procedure. It is anticipated that the developed PDA/protein G coating method will extend the range of applicable materials for biosensing. 相似文献