Liquid chromatographic analysis of prednisolone, prednisone and their 20-reduced metabolites in perfusion media

A reversed-phase liquid chromatographic assay was developed to quantitate prednisolone, prednisone and the 20 alpha-dihydro and 20 beta-dihydro reduced metabolites of both parent compounds in tissue culture media from in vitro perfusions of the human placental lobule. Steroids were extracted from perfusate, using reversed-phase cartridges, with average recoveries of 95.2% or greater. The internal standard for the analyses was 6 alpha-methylprednisolone. In this assay cortisol coelutes with prednisolone, however, no other significant interferences were found. Assay of each steroid was linear in the range 0-1 microgram/ml. Intra-assay coefficients of variation were measured at 10 and 750 ng/ml with ranges of 3.4% (20 alpha-dihydroprednisone) to 8.8% (20 beta-dihydroprednisolone) and 4.1% (20 beta-dihydroprednisone) to 8.8% (prednisone). The corresponding inter-assay coefficients of variation were 3.3% (20 alpha-dihydroprednisone) to 9.1% (20 beta-dihydroprednisolone) and 1.9% (prednisolone) to 3.5% (prednisone). The analyses utilized two C18 columns which were linked together and maintained at 40 degrees C.     

A microeconomic simulation model of reproduction

Translated from Programmnoe Oborudovanie i Voprosy Prinyatiya Reshenii, pp. 152–184, 1989.     

Luminescence of heterogeneous dimers of certain polymethine dyes in dichloroethane

Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 55, No. 5, pp. 881–884, November, 1991.     

Symmetries and conservation laws of Navier-Stokes equations

All the symmetries and conservation laws of Navier-Stokes equations are calculated.     

Behavior of massless feynman integrals near singular points

Nizhnetagil' State Pedagogical Institute. Translated from Teoreticheskaya i Matematicheskaya Fizika, Vol. 80, No. 3, pp. 372–380, September, 1989.     

Inner continuation of regular functions and a problem of kaufman

Translated from Ukrainskii Matematicheskii Zhurnal, Vol. 41, No. 3, pp. 408–412, March, 1989.     

Thin-layer chromatography of radioactively labelled cholesterol and precursors from biological material

Summary The investigation methods of the action of xenobiotics on sterol biosynthesis from ¹⁴C-acetate in rat hepatocyte cultures can be developed, with regard to extraction using Extrelut and the separation of the sterol pattern by thin-layer chromatography, in such a way that they are suitable for wider application, e.g., screening. Good visualisation and recognition of changes in the sterol pattern are possible using autoradiography of the thin-layer chromatogram.

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Dünnschicht-Chromatographie von radioaktiv markiertem Cholesterin und Vorstufen aus biologischem Material. Eine einfache und empfindliche Methode zur Untersuchung einer Beeinflussung des Sterolbiosynthesewegs

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Dedicated to Dr. E. Roesch on occasion of his 65th birthday