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101.
102.
The need for nucleic acid based diagnostic tests has increased enormously in the last few years. On the one hand, this has been stimulated by the discovery of new hereditary genetic disease loci following the completion of the Human Genome Project, but also by the presence of new rapidly spreading viral threats, such as that of the SARS epidemic, or even micro-organisms released for the purpose of biological warfare. As in many instances rapid diagnoses of specific target genetic loci is required, new strategies have to be developed, which will allow this to be achieved directly at the point-of-care setting. One of these avenues being explored is that of biosensors. In this review, we provide an overview of the current state of the art concerning the high-throughput analysis of nucleic acids, and address future requirements, which will hopefully be met by new biosensor-based developments.  相似文献   
103.
A significant influence of the dynamic ergodic divertor (DED) on the density limit in TEXTOR has been found. In Ohmic discharges, where without DED detachment normally arises at the density limit, a MARFE (multifaceted asymmetric radiation from the edge) develops when the DED is operated in a static regime. The threshold of the MARFE onset in the neutral beam heated plasmas is increased by applying 1 kHz ac DED at the high-field side. The theoretical predictions based on the parallel energy balance taking poloidal asymmetries into account agree well with the experimental observation.  相似文献   
104.
We report on the observation of sympathetic cooling of a cloud of fermionic 6Li atoms which are thermally coupled to evaporatively cooled bosonic 87Rb. Using this technique we obtain a mixture of quantum-degenerate gases, where the Rb cloud is colder than the critical temperature for Bose-Einstein condensation and the Li cloud is colder than the Fermi temperature. From measurements of the thermalization velocity we estimate the interspecies s-wave triplet scattering length |amx|=20(+9)(-6)aB. We found that the presence of residual rubidium atoms in the |2, 1> and the |1, -1> Zeeman substates gives rise to important losses due to inelastic collisions.  相似文献   
105.
Within the last year of TEXTOR operation a major part of the experiments were dedicated to the interaction of the ergodized plasma edge, induced by the dynamic ergodic divertor (DED), with the core plasma. Systematic experiments are performed in the 3/1 configuration of the DED. Depending on , the DED generates a locked 2/1 mode in the plasma at a critical current in the DED coils. Different diagnostics were used to measure the plasma response in the core and edge. The investigations in this paper are focused on the measurement of density fluctuation and the analysis of the coherent-mode structure outside the q = 2 surface using O-mode poloidal correlation reflectometry. A reduction of the quasi-coherent mode amplitude and frequency is observed, caused by the 2/1 mode together with a decrease of the density scale length. Moreover, the deduced poloidal rotation velocity at the q = 3 surface changes from the electron to the ion diamagnetic drift direction with the onset of the 2/1 island. Also a reduction of the density fluctuations is observed as well as a decrease in the poloidal correlation length. From Ar-injection a delayed inward propagation of the emission maximum is observed for different Ar ionization stages when the 2/1 mode is generated.Similar experiments with a slowly rotating DED show that the steepened density gradients are spatially localized and confirm the hypothesis that the 2/1 mode is responsible for the observations.Presented at the Workshop Electric Fields Structures and Relaxation in Edge Plasmas, Nice, France, October 26–27, 2004.  相似文献   
106.
The 2s-->3s transition of (6,7,8,9)Li was studied by high-resolution laser spectroscopy using two-photon Doppler-free excitation and resonance-ionization detection. Hyperfine structure splittings and isotope shifts were determined with precision at the 100 kHz level. Combined with recent theoretical work, the changes in the nuclear-charge radii of (8,9)Li were determined. These are now the lightest short-lived isotopes for which the charge radii have been measured. It is found that the charge radii monotonically decrease with increasing neutron number from 6Li to 9Li.  相似文献   
107.
108.
A novel MAS NMR approach is presented for the determination of heteronuclear dipolar couplings in unoriented materials. The technique is based on the proton-detected local field (PDLF) protocol, and achieves dipolar recoupling by R-type radio-frequency irradiation. The experiment, which is called R-PDLF spectroscopy, is demonstrated on solid and liquid-crystalline systems. For mobile systems, it is shown that the R-PDLF scheme provides better dipolar resolution as compared to techniques combining conventional separated local field (SLF) spectroscopy with R-type recoupling.  相似文献   
109.
A novel scheme for all-optical image processing is suggested, which is based on spontaneous pattern-formation processes. The spatiotemporal instabilities, connected with these self-organization effects, appear in many nonlinear optical systems and mostly obstruct the intended application. We propose to utilize the particular features of pattern formation for parallel image processing, namely the sensitivity to external influences, the selection of well-defined final states, and the rotational and translational invariance. We report here on a single-feedback experiment with a liquid crystal light valve as the optical nonlinearity. In this experiment, the recognition of hexagonal structures is realized. We characterize the response dynamics and study associative properties of this scheme. The extension to square patterns is discussed, and first steps towards a practical implementation are undertaken in providing a simple post-processing scheme and testing the concept on realistic input images. Received: 26 November 2002 / Revised version: 7 February 2003 / Published online: 9 April 2003 RID="*" ID="*"Corresponding author. Fax: +49-6151/16-4123, E-mail: ralph.neubecker@physik.tu-darmstadt.de  相似文献   
110.
A pulse sequence is described for the recoupling of heteronuclear dipolar interactions under MAS. The method is similar to the PISEMA experiment, but employs a well-defined amplitude modulation of one of the two radio-frequency fields. The technique is used for measurements of 1H-13C dipolar couplings in unoriented solid and liquid-crystalline samples.  相似文献   
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