光导纤维胆固醇生物传感器的研究

将胆固醇酯酶、胆固醇氧化酶和辣根过氧化物酶通过戊二醛交联反应,固定在牛血清蛋白上,制成光纤胆固醇生物传感器的传感膜.此传感器偶合了胆固醇酯水解、胆固醇的氧化和鲁米诺同过氧化氢的化学发光等3种酶催化反应,通过光纤输出的化学发光信号进行检测.测定总胆固醇和自由胆固醇的线性范围均为0.5～20μg/mL,检测下限为0.1μg/mL,响应时间为2min,寿命在2个月以上,适用于血清中总胆固醇和游离胆固醇的测定.     

分子印迹固相萃取-化学发光测定盐酸金霉素

分子印迹聚合物;固相萃取;化学发光;流动注射;金霉素     

以马铃薯组织为酶供体化学发光新方法测定血清中游离多巴胺

基于超常氧化态的Cu(III)配合物K5[Cu(HIO6)2]能催化低浓度的鲁米诺一过氧化氢化学发光体系,用以测定痕量的过氧化氢;利用马铃薯组织作为酶的提供体.建立了一种测定多巴胺的新方法.多巴胺在马铃薯组织中的多酚氧化酶催化下被溶解氧氧化产生过氧化氢,通过测定产生的过氧化氢间接地测定多巴胺的浓度.实验表明,相对发光强度与多巴胺浓度在2.1×10-10～2.1×10-6mol·L-1范围内呈良好的线性关系,检出限为7.2×10-11mol·L-1.对3.0×10-9mol·L-1多巴胺平行测定7次,相对标准偏差为2.1%.该方法具有极高的灵敏度,可用于直接测定正常人血清中的游离多巴胺的浓度.     

液芯波导阵列芯片检测血清及尿液中的β2-微球蛋白

提出了一种液芯波导免疫分析阵列芯片的检测系统,结合光强差技术提高了ELISA的灵敏度,HRP的线性范围为1×10-10~9×10-10g/L,检出限为3.0×10-11g/L,RSD小于1%(c=3×10-10g/L,n=11),线性范围的下限与检出限均比普通的光度法下降了100倍,将其应用于血清和尿液中β2-微球蛋白的免疫分析,与标准方法所得结果相符。     

Construction of a Family of Maximally Entangled Bases in ℂd ⊗ ℂd′

In this paper, we present a new method for the construction of maximally entangled states in Cd⊗Cd′ when d′≥2d. A systematic way of constructing a set of maximally entangled bases (MEBs) in Cd⊗Cd′ was established. Both cases when d′ is divisible by d and not divisible by d are discussed. We give two examples of maximally entangled bases in C2⊗C4, which are mutually unbiased bases. Finally, we found a new example of an unextendible maximally entangled basis (UMEB) in C2⊗C5.     

流通式化学发光传感器测定汽油中的铅(ⅱ)

将鲁米诺(luminol)和高锰酸根同时固定在阴离子交换树脂柱中,磷酸盐缓冲溶液既作为洗脱剂洗脱树脂中的离子,又作为碱性介质,引起KMnO4氧化Luminol产生化学发光,在铅的作用下在样品池中产生后续化学发光,建立了一种测定铅的流通式化学发光传感器.传感器的线性响应范围为5.0×10-8～1.0×10-5g/mL,检出限为3×10-8g/mL(3σ).整个分析过程可以在1 min内完成.对2.0×10-6g/mL的样品进行9次平行测定,其相对标准偏差为3.2%.传感器在200 h内可以重复使用300次.应用于汽油中铅(Ⅱ)的测定结果令人满意.     

Protecting group-free total synthesis of (-)-lannotinidine B

The first total synthesis of (-)-lannotinidine B, a unique tetracyclic constitutent of Lycopodium annotinum, has been accomplished in 10 steps with 23% overall yield. The completed short and efficient synthesis is characterized with three highly chemo- and/or stereoselective reductive-amination steps to furnish the desired trans-fused 6/6 bicycle and the aza seven-membered ring system, and a direct intramolecular acyloin condensation to deliver the cyclopentanone moiety, as well as successful application of a protecting group-free strategy and an optimal redox order.     

Enantioselective total synthesis of marine diterpenoid clavulactone

The key steps in the synthesis of clavulactone are formation of an enantiopure cyclopentane precursor by epoxide rearrangement and intramolecular carbonyl-ene reaction, construction of the 3,4-dihydro-2H-pyran ring by intermolecular hetero-Diels-Alder reaction, closure of the eleven-membered ring, and finally generation of the lactone functionality by chemoselective allylic C(sp(3))-H oxidation.     

A {Ni7} cluster-containing sandwich-type phosphotungstate functionalized by organic bisphosphonate ligands and its two-dimensional supramolecular structure

A {Ni₇} cluster-substituted sandwich-type phosphotungstate Na₁₀（NH₄）₄[{(B-a-PW₉O₃₄)Ni₃ （OH）（H₂O）₂（HAIe）}₂Ni]·22H₂O（1） was syntliesized using the conventional aqueous solution method. Compound 1 represents the first nickel-containing sandwich-type polyoxometalate functionalized by bisphosphonate ligands.Fuither,the sandwich-type polyoxoanions aggregated via the H-bonding interactions into a two-dimensional（2D） supramolecular framework.     

New cytotoxic annonaceous acetogenin mimetics having a nitrogen-heterocyclic terminal and their application to cell imaging

A terminal unsaturated lactone or its equivalent is commonly believed to be essential for the cytotoxicity of natural annonaceous acetogenins and their artificial mimetics. In this work, we discovered a series of new cytotoxic ethylene glycol ether-containing mimetics, in which a variety of simple aliphatic nitrogen-heterocycles were introduced to replace the lactone terminal of AA005 (1), a representative bioactive polyether mimic identified from our previous research, for the first time. Among these, mimic 4 bearing a terminal piperazine was found to be the most potent compound against the proliferation of three cancer cells. Based on our new findings, a fluorescent probe 7 was also developed and successfully applied to the imaging of cancer cells. This work provides a new strategy for developing simpler cytotoxic mimetics of natural annonaceous acetogenins and molecular tools for biological imaging.