“Clickable” affinity ligands for effective separation of glycoproteins

In this paper, we present a new modular approach to immobilize boronic acid ligands that can offer effective separation of glycoproteins. A new “clickable” boronic acid ligand was synthesized by introducing a terminal acetylene group into commercially available 3-aminophenyl boronic acid. The clickable ligand, 3-(prop-2-ynyloxycarbonylamino)phenylboronic acid (2) could be easily coupled to azide-functionalized hydrophilic Sepharose using Cu(I)-catalyzed 1,3-dipolar cycloaddition reaction under mild condition. Compared to other boronic acid affinity gels, the new affinity gel displayed superior effectiveness in separating model glycoproteins (ovalbumin and RNase B) from closely related bovine serum albumin and RNase A in the presence of crude Escherichia coli proteins. Because of the simplicity of the immobilization through “click chemistry”, the new ligand 2 is expected to not only offer improved glycoprotein separation in other formats, but also act as a useful building block to develop new chemical sensors for analysis of other glycan compounds.     

水滑石负载钯催化剂上的醇无氧脱氢反应

 研究了多种载体负载 Pd 催化剂上苯甲醇无氧脱氢反应. 结果发现, 以兼具较强酸性和碱性的水滑石 (HT) 为载体时, Pd 催化剂具有优异的苯甲醇转化活性和苯甲醛选择性, 当 Pd 含量为 0.32%~0.55% 时催化性能最佳. Pd/HT 催化剂可重复使用, 且对于含推电子取代基的芳香醇、2-噻吩甲醇、α,β-不饱和醇与环状脂肪醇等的直接脱氢反应均具有较好催化性能. HT 表面的 Pd(II) 物种反应后转变为平均粒径为 2.0~2.5 nm 的 Pd 纳米粒子或纳米簇. 具有较高分散度的 Pd(II) 物种易转变为较小的 Pd 纳米粒子, 从而具有较佳的催化性能. 本文推测, 催化剂表面的碱性位可促进苯甲醇 O–H 键的活化, 形成 Pd-苯甲氧基中间体, 该中间体进一步脱氢生成苯甲醛和 Pd-H 物种; 而催化剂表面的质子酸位可与 Pd-H 作用, 促进 H2 的脱除.     

气相色谱-质谱法测定玩具中的4种阻燃剂

建立了玩具中三(2-氯乙基)磷酸酯、磷酸三邻甲苯酯、2,2′,4,4′,5-五溴联苯醚、2,2′,3,3′,4,4′,6,6′-八溴联苯醚等4种阻燃剂的气相色谱-质谱分析方法。玩具样品以V(正己烷):V(二氯甲烷)=1:1混合溶液为提取溶剂,超声提取30min,提取液以DB-5HT石英毛细管色谱柱(15m×0.25mm×0.10μm)分离后进行选择离子监测模式下的定性及定量分析。4种阻燃剂的定量限为0.1mg/kg,在低、中、高3个添加水平范围内,4种阻燃剂的平均回收率为93.5%～97.3%,相对标准偏差在2.4%～4.1%之间。     

平面场景和建筑物成像的几何分析

为了识别采集图像中的平面场景以及建筑物,利用摄像机参数,对平面场景及建筑物成像过程进行了几何分析。首先,根据相机参数以及相机飞行状态分析平面场景成像畸变;然后,对平面场景上的建筑物形状的成像进行几何分析;最后,将相机上采集到的平面场景及建筑物的图像像素数与真实平面场景及建筑物的尺寸建立联系。实验结果表明:已知相机参数及飞行状态的情况下,可以对平面场景及建筑物形状信息进行推导,从而完成目标识别与跟踪。本方法适合在典型建筑物、孤立建筑物、建筑群中具有明显形状特征等情况下对立体目标进行自动捕获与跟踪。     

Multi-wavelength mode-locked Er/Yb Co-doped fiber laser with square nano-second pulse output

We report a multi-wavelength mode-locked Er/Yb codoped fiber laser with square nano-second pulse output, which is constructed with a “figure-of-eight” cavity structure. A section of polarization-maintaining Er/Yb co-doped double clad fiber was used as the gain medium and pumped by a fiber pigtailed multimode laser diode working at 975 nm via a multimode fiber combiner. Several megahertz repetition rate output pulses with peak power up to 9.2 W and pulse duration around several nanoseconds were obtained.     

Pivoting in Extended Rings for Computing Approximate Gr?bner Bases

It is well known that in the computation of Gr?bner bases arbitrarily small perturbations in the coefficients of polynomials may lead to a completely different staircase, even if the solutions of the polynomial system change continuously. This phenomenon is called artificial discontinuity in Kondratyev’s Ph.D. thesis. We show how such phenomenon may be detected and even “repaired” by using a new variable to rename the leading term each time we detect a “problem”. We call such strategy the TSV (Term Substitutions with Variables) strategy. For a zero-dimensional polynomial ideal, any monomial basis (containing 1) of the quotient ring can be found with the TSV strategy. Hence we can use TSV strategy to relax term order while keeping the framework of Gr?bner basis method so that we can use existing efficient algorithms (for instance the F ₅ algorithm) to compute an approximate Gr?bner basis. Our main algorithms, named TSVn and TSVh, can be used to repair artificial e{\epsilon}-discontinuities. Experiments show that these algorithms are effective for some nontrivial problems.     

Direct mass measurements of short-lived A=2Z-1 nuclides (63)Ge, (65)As, (67)Se, and (71)Kr and their impact on nucleosynthesis in the rp process

Mass excesses of short-lived A=2Z-1 nuclei (63)Ge, (65)As, (67)Se, and (71)Kr have been directly measured to be -46,921(37), -46,937(85), -46,580(67), and -46,320(141) keV, respectively. The deduced proton separation energy of -90(85) keV for (65)As shows that this nucleus is only slightly proton unbound. X-ray burst model calculations with the new mass excess of (65)As suggest that the majority of the reaction flow passes through (64)Ge via proton capture, indicating that (64)Ge is not a significant rp-process waiting point.     

Optical characteristics of transparent PMNT ceramic and its application at high speed electro-optic switch

The optical characteristics of transparent lead magnesium niobate titanate (PMNT) electro-optic ceramic, including the electro-optic phase modulation, electric hysteresis property and thermo-optic coefficient, are investigated in detail. Based on this novel ceramic, a polarization independent electro-optic switch by using fiber Sagnac interferometer (FSI) structure is realized. An initial π-shift is introduced into the Sagnac loop to eliminate the effect of the polarization orientation of the incident light on the switch performance. Then an electrically controllable PMNT phase retarder is used to switch the optical signal between the reflection and transmission ports. Some theoretical analyses are given and the switch performances are also discussed, including the thermal characteristic and different switching frequency response.     

Deterministic quantum dense coding with cluster state in optical systems

We propose two optical schemes for implementing the deterministic single-particle and two-particle quantum dense coding using four-qubit cluster states. In the protocols, the photon is neuter particle, so it has longer decoherence time with the environment than other particles. It is easy to implement single-bit gate using the linear optical elements under certain conditions, so the transformations performed on the photons by Alice can be easily achieved. Here the cluster states can be exactly discriminated using the parity detector, PBS and FS-PBS. In addition, the success probabilities of the dense coding are both equal to 1.     

Reflection chromaticity of cholesteric liquid crystals with sandwiched periodical isotropic defect layers

We study one-dimensional photonic crystals made of cholesteric liquid crystals with sandwiched isotropic defect layers. Based on the Berreman Fast 4 × 4 matrix method, the dispersion relation of one-dimensional photonic crystals is calculated and the corresponding reflection chromaticity is obtained. It is found that the color shift could be controlled by adjusting the thickness and refractive index of the isotropic defect layers. Compared with conventional structures, the reflection chromaticity of this structure is insensitive to the incident angle, if the thickness ratio of the cholesteric liquid crystals to that of the isotropic defect layers and the refractive index of periodical isotropic defect layers are properly set. Furthermore, the common forbidden bands for both left and right circular polarizations can be obtained, and we also take the wavelength-dependent refractive indices into consideration and obtain the reflected light chromaticity with the incident angle increasing. The proposed device can be used as a reflective color filter in the display industry.