8,2'-O-环化-9β-D-腺嘌呤阿拉伯糖苷的NMR及结构研究

8,2'-O-环化-9β-D-腺呤阿拉伯糖苷是阿糖腺苷合成过程中的一个关键性中间体,该化合物的NMR数据只有¹H-NMR谱的糖环1'位质子化学位移和偶合常数的报道,¹³C-NMR谱未曾报道。本文用2D-NMR、NOE-DIF等技术,对它的¹H、¹³C-NMR谱进行归属,进一步明确了它的结构。     

In situ spectral imaging of marker proteins in gastric cancer with near-infrared and visible quantum dots probes

This study presents the investigation of bioconjugating ability of near-infrared (NIR) CdSeTe/ZnS quantum dots (QDs) (710 nm) and visible CdSe QDs (595 nm) in immunofluorescent staining for cancer biomarkers in gastric cancer tissues probed with the homemade Hadamard transform (HT) spectral imaging microscope and a commercial multispectral imaging system. The results show that imunostaining ability of NIR QDs probes is stronger than that of visible QDs when the two kinds of QDs are simultaneously used to probe the cancer biomarkers such as cytokeratin 20 (CK20) and proliferating cell nuclear antigen (PCNA) in gastric cancer tissues. Moreover, when the two QDs probes are used for immunostaining successively for the same target molecules, staining order has great influences on the final results due to their different conjugating ability to the marker proteins. The results imply that NIR QDs hold more promise for real-time imaging of tumor tissues due to its higher sensitivity and contrast. In addition, the results also demonstrate the potential of Hadamard transform spectral imaging as a useful tool in biomedical analysis and quantitative evaluation for tumor tissues.     

样条的机械化求解

本文在逐次分解法的基础上，给出一种样条机械化求解方法．该方法对多项式样条，有理样条乃至更一般样条的研究都是十分有效的．它适用于三角剖分，矩形剖分乃至更一般的代数曲线剖分     

Interpolation algorithm for recovering the missing phase values in 3D measurement

In the three-dimensional (3D) phase measurement, some marks are usually adhered to the object in order to make the 3D registration process faster and easier. As covered with marks, local phase data are missing and have to be interpolated later. Considering the phase distribution nearby the marks, a gradient estimate (GE) interpolation algorithm is provided here. This algorithm recovers one pixel's missing phase value with the average of the estimated values which is calculated by gradients in eight directions nearby. Since this algorithm is a local processing, the missing phase values should be interpolated from the edge of the marks to the center. In the computer simulation and the practical experiment, compared with the same-size neighborhood mean (NM) algorithm and the Gerchberg-Saxton (GS) algorithm, this new algorithm achieves very good fit results with the least time. So it can be used as a practical tool for automatic missing phase interpolation.     

Topography guiding the accelerated and persistently directional cell migration induced by vaccinia virus

The rapid transmission of vaccinia virus(VACV)in vivo is thought to be closely related to the cell migration induced by it.Cell migration involved in dynamic changes of cell-substrate adhesion and actin cytoskeleton organization,which can influence by the micro/nano-scale topographic structures that cells are naturally exposed to via contact guidance.However,migration behaviors of VACV-infected cells exposed to topographic cues are still unknown.Herein,we designed an open chip with microgrooved poly(dimethyl siloxane)(PDMS)substrate to explore the topography roles in VACV-induced cell migration.Differed from the random cell migration observed in traditional scratch assay on planar substrate,VACV-infected cells had a tendency to persistently migrate along the axis parallel to microgroove with increased velocity.Moreover,infected cells exhibited a dominant elongated protrusion aligned to the micro-grating axis compare to the shorter lamella extended in any direction on smooth substrate.Interestingly,the Golgi complex preferred to relocate behind the nucleus confined within the micro-grating axis in majority of infected migratory cells.The directional polarization of cells embodied in protrusion formation and Golgi reorientation was responsible for the directionally persistent migration behaviors induced by VACV on microgrooved substrate.Infected cells response to substrate topography,causing the actin-filled stretched protrusion containing numerous virions and accelerated movement is likely to facilitate direct and rapid spread of VACV.This work opens a window for us to understand the migration behaviors of infected cells in vivo,and also provides a cue for revealing the relationship between virus-induced cell migration and virus rapid spread.     

Investigations Ce Doped MnO<Subscript>2</Subscript>/rGO as High Performance Supercapacitors Material

The novel Ce doped MnO₂/rGO composite was fabricated by a simple two-step hydrothermal method reacted for different times. As results, the composite reacted for 1h exhibits better electrochemical performance and rate capacity, the capacitive retention is 85% from 130.44 F g^–1 for first cycle decrease to 111.11 F g^–1 after 1000 cycles at the current density of 1 A g^–1, and 14.7 W h kg^–1 of energy density. Moreover, the BET surface area is 243 m² g^–1, and the average pore size is 7.9 nm, which will be convenient for the quick transport and migration of electrolyte ions during the charge–discharge process, and further confirm good rate capability.     

Polarimetric-Spectral-Sensitive Compressed Ultrafast Photography Enables Real-Time Intensity Visualization of Time-Varying Polarized Ultrafast Laser Pulses

Ultrafast laser pulses with complex spatiotemporal and polarization couplings have shown great application prospects in coherent control, particle acceleration, molecular dynamics excitation, and chiral or anisotropic detection. It is very important to observe the polarization-coupled ultrafast laser pulses for characterizing the performance of laser source and understanding the laser-matter interaction, but there is currently no detection technique that can completely characterize them, including the spatio-, spectral-, and polarimetric-temporal information. In this work, a polarimetric-spectral-sensitive has been compressed ultrafast photography (PS-CUP) to realize the real-time intensity visualization of time-varying polarized (TP) ultrafast laser pulses. PS-CUP can not only distinguish the polarization state of a TP pulse, but also realize the spatio-, spectral- and polarimetric-temporal measurement. To demonstrate the ability of polarization resolving detection, PS-CUP is used to successfully distinguish between the sinistral and dextral TP pulses and measure the angle and degree of linear polarization of a dextral TP pulse, respectively, and the experimental results are highly consistent with theoretical simulations. Owing to the unprecedented multidimensional detection ability, PS-CUP is prospected to provide a powerful tool for the diagnosis of various complex ultrafast laser pulses and the detection of ultrafast chiral optics.     

2,5-双取代-1,3,4-噁二唑类化合物的合成与表征

以3-( 4-氟苯基) -1H-吡唑-5-甲酸乙酯为原料,与H2NNH2?H2O发生肼解生成3-( 4-氟苯基) -1H-吡唑-5-甲酰肼,再与CS2环化生成2-巯基噁二唑中间体,最后在巯基上进行烷基化反应合成了一系列新型的2-硫醚-5-吡唑基-1,3,4-噁二唑类化合物,并利用IR、1H NMR、HRMS等波谱技术对目标化合物结构进行了表征。该合成方法具有原料易得,后处理简便,收率较高的优点。     

Internal supply chain coordination in the electric utility industry

Electric utilities have a well-established vertical internal and external supply chain. Theoretically, information sharing, involving production, inventory, and other policy variables, between chain entities can improve supply chain performance. Such sharing relies on a systematic determination of the optimal policy variables for the chain. This paper presents an analysis of the electric utility’s decision problem based on an optimal control model in a competitive market environment. Optimal production and inventory policies are developed for a centralized supply chain under full information sharing. The model and policies are tested with electric utility industry data, and performance implications are discussed for electric utility managers and public regulators.