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111.
112.
The gene fragment (191 bp) encoding protein G IgG Fc binding domain was isolated by PCR from group G streptococcus (CMCC32138), and a clone containing this gene fragment was found to give fine reactivity to human IgG when expressed in Escherichia coli. The complete nucleotide sequence of the gene fragment was determined. One base pair differs from previously reported protein Gnucleotide sequences, and resultsin an amino acid change (Ala-Thr), but this variation makes no difference in binding to the IgG Fc part by ELISA.The secondary structure of the protein G IgG Fc binding domain has been estimated by circular dichroism and assigned by computer algorithm.It shows a typical α-helix region in this domain.By breaking this α-helix region with recombinant DNA techniques, a 44 peptide, which contained the N-terminal 27 amino acid residues of this domain, was expressed in E. coli and showed no reactivity to IgG.The hydropathicity of this domain was also analyzed and compared with that of protein A relevant 相似文献
113.
EuMO3的XPS和Mossbauer谱研究 总被引:3,自引:0,他引:3
对EuMO3进行了XRD、XPS、IR和^151Eu Mossbauer谱测量,研究了晶胞参数与离子半径、Eu4d光电子峰的结合能、同质异能移位的关系。结果表明,Eu4d结合能和同质异能移位均与B位离子M的变化有关,且结合能与同质异能移位成线性关系。 相似文献
114.
用3TW飞秒激光器研究了激光-固体靶相互作用中产生的超热电子的能量分布.超热电子构成各向异性的能量分布:在靶法线方向,超热电子能谱呈类麦克斯韦分布,拟合的温度约为206keV,该方向占主导地位的加速机理是共振吸收;在激光反射方向,超热电子能谱先是出现一个局部的平台,然后逐渐衰减,呈现非类麦克斯韦分布,这是由于几种加热机理共同作用的结果,其中占主导地位的是反射激光对电子的加速.在靶法线方向超热电子的温度和产额均大于激光反射方向超热电子的温度和产额,证明共振吸收机理对电子的加速更有效.
关键词:
飞秒激光
等离子体
超热电子
能谱 相似文献
115.
Five new-type Fc receptor molecules were constructed based on streptococcal protein G (SpG) and staphylococcal protein A (SpA). These protein molecules contain one to six Fc binding domains to immunoglobulins which are structurally different from native SpG or SpA. Their expression levels reached 17-30% of the total bacterial proteins after heat induction in E. colt. Immunodiffusion and ELISA results showed that the engineered protein TG (184 amino acid residues) composed of three SpG C3 domain could bind more broadly and efficiently than the native SpG to the IgGs of human, goat, rabbit, etc. , and its optimal pH for binding became wider (pH5-8) compared with the SpG (pH5) ; and the protein TGA (357AA), fused by protein TG and the A, B, C domains of SpA, displayed both the binding pattern of SpG and SpA. 相似文献
116.
在含水甲苯中,对甲基氯苄与锡粉反应合成了二对甲基苄基二氯化锡,将其分别与2-羰基丙酸苯甲酰腙及2-羰基丙酸水杨酰腙反应,合成了2个取代苄基锡配合物(1、2),通过元素分析、IR、1H NMR、13C NMR、X射线单晶衍射以及热重分析等表征了配合物结构。测试了配合物对癌细胞MCF-7、Hep G2、NCI-H460以及正常人体肝细胞HL-7702的体外抑制活性;在Tris-HCl缓冲溶液中,以EB作为荧光探针,用荧光光谱法初步研究了配合物与小牛胸腺DNA的相互作用。结果表明:配合物1、2对3种癌细胞都有明显的抑制作用,配合物2对HL-7702的细胞毒性小于1;配合物1与小牛胸腺DNA作用是插入结合与静电结合共同作用所致,配合物2与小牛胸腺DNA作用是插入结合作用所致。 相似文献
117.
In this study, liquid crystal (LC) was used to study the interactions of alkyl polyglycosides (APG) with gelatin and bovine serum albumin (BSA) at the LC-aqueous interface. The LC easily undergo an orientational transition from a homeotropic to a planar state after proteins were in contact to the LCaqueous interface decorated with APG, thus inducing an optical change from dark to bright. The optical image analysis reveals that the rearrangement rate of APG monolayer is tightly dependent on the concentration and chemical structure of the protein of interest. For example, the rearrangement rate of APG monolayer increases with an increasing gelatin concentration. We also find that the chemical structure of the proteins has a significant impact on the difference in the growth behavior of bright domains in LC. 相似文献
118.
In this paper the master equation method is used to calculate the
relaxation and decoherence times of a qubit. The results are beyond
Markovian approximation, where the noise spectrum is assumed to be
wide-band, so that they are valid for not only the wide- but also
the narrow-band noises, which may be the main decoherence source in
solid-state qubits. Moreover, for some special cases, analytical
results can be achieved, which are consistent with those derived by
others. 相似文献
119.
采用共沉淀-浸渍法在不同载体焙烧温度下,制备了不同Al/Ba原子比的Pt/BaAl2O4-A12O3系列样品。用XRD,XANES,EXAFS,以及NSC(NO_x storage capacity)测定等手段对样品的微观结构和NO_x储存性能进行了详细的表征.样品中Ba物种是以BaAl2O4和BaCO3两种混合物相的形式存在,且伴随着载体焙烧温度和Ba含量的降低,BaAl2O4物相的分散度变高,NO_x储存活性也随之提高,这表明BaAl2O4相的分散度与样品的NO_x储存性能密切相关,小颗粒的BaAl2O4相是NO_x的主要储存活性中心.在样品中,Pt物种以金属原子簇形式存在.分散度很高,其Pt—Pt壳层配位数较标样Pt粉有显著下降,Pt—Pt键长变短,出现了纳米收缩现象.高分散的小颗粒金属Pt原于簇为捕获和氧化NO_x的主要活性中心. 相似文献
120.