诺氟沙星与DNA的拉曼光谱研究

报道了诺氟沙星(NFX)及诺氟沙星胶囊内容物的FT-Raman光谱和在银胶基底上的表面增强拉曼光谱(SERS),归属了各个振动;研究了诺氟沙星与DNA的相互作用的SERS,结果表明:胶囊内容物的拉曼光谱图与对照品的拉曼光谱图的特征振动峰:C-F键的伸缩振动,C=C伸缩振动,O-C-O的对称伸缩振动峰值未发生变化,发生变化主要是分子骨架振动峰,诺氟沙星胶囊的辅料对拉曼光谱无实质影响,可建立拉曼光谱法检测诺氟沙星药物的分析方法;诺氟沙星可以在没有金属离子的存在下与DNA直接作用,与DNA相互作用的主要键合模式是插入作用,NFX分子中的平面结构插入DNA的双螺旋碱基平面,为深入了解喹诺酮类抗生素的抗菌机理提供可靠依据.     

矿化剂对铝酸钙粉的结构与光谱性能的影响

研究了矿化剂对制备铝酸钙粉的结构与性能的影响.研究中以铝矾土和石灰石为原料,加入一定量的矿化剂,混合均匀,经高温煅烧制得铝酸钙粉.借助于红外光谱、X射线粉末衍射、差热-热重分析等手段对不同原料的铝酸钙粉的结构、组成及性能进行了表征和研究,并对矿化剂在合成铝酸钙中的矿化机理进行探讨.研究表明:加入矿化剂后,可在更低温度条件下煅烧制备出铝酸钙粉,有利于节能减排.红外光谱分析、XRD分析、DTA-TG分析显示:未加矿化剂煅烧时,生成的产物主要是Ca3Al10O18,CaAl2Si2O8.加入矿化剂后煅烧生成的产物主要是易浸出Al2O3的CaAl3BO7和Ca3Al10O18,而不易浸出Al2O3的CaAl2Si2O8的含量大大降低.同时显示,加入矿化剂后,原料中的方解石(CaCO3)分解更容易;CaCO3与铝矾土反应更充分;更有利于促使铝矾土中的Al-Si键断裂,将铝矾土中的Al释放出来;并可降低铝酸钙的煅烧温度.研究还显示:加入矿化剂后,可以改变原产物的晶体结构与成分,有利于降低煅烧反应温度.     

庆大霉素与水溶性CdTe量子点的荧光共振能量转移作用

以巯基丙酸(mercaptopropionic acid,MPA)为稳定剂合成水溶性CdTe最子点(quantum dots,QDs),以CdTe QDs作为能量供体.庆大霉素(Gentamycin,GT)作为能垦受体,建立了荧光共振能量转移(fluorescence resonance energy transfer,FRET)体系.在690 nm处可见发射峰,半峰宽约10 nm,在一定范围内荧光强度与GT的含量旱线性关系,线性范围为2～20 mg·L-1,相关系数r=0.986 7.优化了不同的激发波长、pH、离子强度、时间和温度等凼素对反应的影响,并应用傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)和高效液相色谱(high-performance liquid chromatography,HPLC)分别表征了化学结构和相对专一性.结果表明巯基丙酸的巯基中S原子和羧基中氧原子与纳米微粒表面的富Cd离子发生了配位作用,CdTe QDs与GT的耦合主要是通过量子点周围巯基丙酸羧基(-COOH)中的氧原子与GT的胺基(-NH2)形成分子问氧键实现的;GT与CdTe QDs的结合率为0.35:1.研究表明GT可以作为检测CdTe QDs标记牛血清白蛋白(bovine serum albumin,BSA)的荧光增敏剂,荧光强度值增强6倍,应用前景广阔.     

ICP等离子体鞘层附近区域发光光谱特性分析

为了独立控制鞘层附近区域离子密度和离子能最分布,采用光发射谱(OES)测量技术,对不同射频功率、放电气压和基底偏压下感应耦合等离子体鞘层附近区域辉光特性进行了研究.原子谱线和离子谱线特性分析表明,在鞘层附近区域感应耦合等离子体具有较高的离子密度和较低的电子温度.改变放电气压和射频功率,对得到的光谱特性分析表明,鞘层附近区域离子密度随射频功率的增大而线性增大,在低压下随气压的升高而增大.低激发电位原子谱线强度增加迅速,高激发电位原子谱线强度增加缓慢,而离子谱线强度增加很不明显.改变基底直流偏压,对得到的发射光谱强度变化分析表明,谱线强度随基底正偏压的增加而增大.随着基底负偏压的加入,谱线强度先减小而后增大;直流偏压为-30 V时,光谱强度最弱.快速离子和电子是引起Ar激发和电离过程的主要能量来源.     

基于物理课堂有效教学的策略浅析

根据有效教学的理念提出了课堂有效教学的基本策略、动力策略和长效策略,并结合基础物理的教学,对这些教学策略的科学掌握和运用进行了探讨.     

超导材料的各向异性特征与超导转变温度T_c

在本文中,以电声子机制超导电性理论为基础,用电负性均衡原理研究了由于超导材料的各向异性引起的电子态密度分布的不均匀性,晶格稳定性的差异性及超导材料中元素成键特征对超导转变温度影响的特征,提出了在各向异性的超导材料中电声子机制可以产生高的超导转变温度Tc.     

Primary result of ~(236)U measurement with accelerator mass spectrometry at CIAE

The rare isotope 236U has a half-life of 2.342(3)×107 years,and is produced principally by thermal neutron capture on 235U.The isotopic atom ratio of 236U/238U depends on the integral thermal neutron flux received by the material of interest.236U is potentially useful as a fingerprint for indicating the presence of neutron-irradiated uranium usually originating from nuclear activity.By extracting negative molecular ion UO- from the uranium oxide target,simulating the 236U16O- beam transport with 238U16O- an...     

Induced electric fields and plasmonic interactions between a metallic nanotube and a thin metallic film

We have numerically simulated the induced electric fields and the plasmonic interactions of a metallic nanotube near a thin metallic film. Our study shows that the energies and intensities of the plasmon resonances depend strongly on the aspect ratio (the ratio of the inner to outer radius) of the nanotube as well as the separation between the center of the nanotube and the upper surface of the metallic film and the thickness of the film. The enhancement of the induced electric field of this system reaches ...     

Research on application of MOOC in optical packet switching

An experiment on receiving and identifying multiple optical orthogonal codes （MOOC） sequence-based optical labels in an optical packet switching （OPS） network is reported. Two groups of MOOC with a code length of 23 and a code weight of 3 are used to identify the optical labels. The scheme and principle of OPS networks based on MOOC sequence-based optical labels are presented. Because optical labels contain OPS and routing information, the importance of optical label processing with multiple inconsecutive, random, and burst optical labels in an OPS network is pointed out. Considering the MOOC-based optical labels, we design a circuit consisting of a broadened network and a cascaded amplifier to receive multiple groups of low-power narrow pulses （optical labels in the MOOC-OPS network） with a period of 2 ns. The successful experimental results demonstrate that the designed scheme is feasible.     

Artificial neural network method for determining optical properties from double-integrating-spheres measurements

<正>Accurate measurement of the optical properties of biological tissue is very important for optical diagnosis and therapeutics.An artificial neural network(ANN)-based inverse reconstruction method is introduced to determine the optical properties of turbid media,which is based on the reflectance(R) and transmittance (T) of a thin sample measured by a double-integrating-spheres system.The accuracy and robustness of the method has been validated,and the results show that the root mean square errors(RMSEs) of the absorption coefficientμ_a and scattering coefficientμ'_s reconstruction are less than 0.01 cm~(-1) and 0.02 cm~(-1),respectively.The algorithm is not only very accurate in the case of a lower albedo(~0.33),but also very robust to the noise of R and T especially for theμ'_s reconstruction.