Two-dimensional protein database of human pancreas

We report here the two-dimensional protein database of human pancreas. The proteins were analyzed by two-dimensional electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Totally, 302 proteins were identified, of which about 27% were enzymes with a broad range of catalytic activities. Several of these are specifically expressed in pancreas, such as pancreatic amylase, pancreatic stone protein, pancreatitis-associated protein, pancreatic lipase, pancreatic elastase, etc. Structural and cytoskeletal proteins are also strongly represented on the gels. Thus, the pancreatic proteome reflects the organ's function. This work paves the way for further studies on pancreatic protein expression in health and disease, such as diabetes and pancreatic cancer.     

吲哚衍生物与花生酸混合LB膜的制备及结构研究

本文将3-三氰基乙烯基-N-十六烷基吲哚与花生酸混合制成单分子膜。不同摩尔比混合膜的π-A曲线均出现平台,表明膜压缩过程中分子排列相态发生变化。混合LB膜的紫外吸收光谱证明吲哚分子形成聚集体,X-射线衍射结果说明这种聚集体被夹带在花生酸的框架结构中。     

DNA-mediated coordinative assembly of upconversion hetero-nanostructures for targeted dual-modality imaging of cancer cells

We reported a simple and universal strategy for DNA-mediated assembly of CdTe quantum dots (QDs) and lanthanide-doped upconversion nanoparticles (UCNPs). Such DNA-QD/UCNPs heterostructures not only maintains both fluorescent properties of QDs and upconversion luminescence behaviors of UCNPs, but also offers a polyvalent DNA surface, allowing for targeted dual-modality imaging of cancer cells using an aptamer     

涂敷法制作质谱场电离发射丝的方法及性能研究

本文介绍一种新的质谱场电离发射丝的制备方法——涂敷法,并研究了其制备机理及影响发射丝灵敏度的因素。此外,还对涂敷法制作的发射丝及高温活化法制作的发射丝进行了各项性能对比试验。最后,介绍了涂敷发射丝的应用效果。     

Zig‐Zag Diphosphene Oligomers Linked by Silver(I) Cation

The coordination of silver cation to diphosphene Mes*P=PMes* ( 1 , Mes* = ^tBu₃C₆H₂) was investigated in detail. The reaction of 1 with Ag[Al(OR_F)₄] (OR_F = OC(CF₃)₃) in the ratios of 2 : 1, 3 : 2 and 1 : 2 led to the formation of the first cationic silver linked diphosphene complexes 2 — 4 . Complexes 2 and 3 contain two and three diphosphene molecules linked by the linear Ag(I) cation, respectively, and they feature unusual zig‐zag topologies. Complex 4 is a dinuclear silver complex, and each Ag(I) center features a tetrahedral geometry, coordinated by one phosphorus atom of diphosphene 1 and three chloro atoms of two CH₂Cl₂ molecules.     

Solution-phase, triangular ag nanotriangles fabricated by nanosphere lithography

A novel method to produce solution-phase triangular silver nanoparticles is presented. Ag nanoparticles are prepared by nanosphere lithography and are subsequently released into solution. The resulting nanoparticles are asymmetrically functionalized to produce either single isolated nanoparticles or dimer pairs. The structural and optical properties of Ag nanoparticles have been characterized. Mie theory and the Discrete Dipole Approximation method (DDA) have been used to model and interpret the optical properties of the released Ag nanoparticles.     

硫酸颜色反应用于荧光法测定利血平研究

提出了硫酸颜色反应用于利血平的荧光分析新方法。利用血平与浓硫酸反应，生成强荧光物质，所得产物的荧光强度与利血平的浓度在０－０．６μｇ／ｍＬ范围内有良好的线性关系。检测限为０．２ｎｇ／ｍＬ。本法可直接用于尿液中利血平的定量分析，回收率为８２．５％－８４．２％。     

Recoverable solution reaction of HiPco carbon nanotubes with hydrogen peroxide

There is increasing interest in developing single-walled carbon nanotubes (SWNTs)-based optical biosensors for remote or in vitro and in vivo sensing because the near-IR optical properties of SWNTs are very sensitive to surrounding environmental changes. Many enzyme-catalyzed reactions yield hydrogen peroxide (H(2)O(2)) as a product. To our knowledge, there is no report on the interaction of H(2)O(2) with SWNTs from the optical sensing point of view. Here, we study the reaction of H(2)O(2) with an aqueous suspension of water-soluble (ws) HiPco SWNTs encased in the surfactant sodium dodecyl sulfate (SDS). The SWNTs are optically sensitive to hydrogen peroxide in pH 6.0 buffer solutions through suppression of the near-IR absorption band intensity. Interestingly, the suppressed spectral intensity of the nanotubes recovers by increasing the pH, by decomposing the H(2)O(2) into H(2)O and O(2) with the enzyme catalase, and by dialytically removing H(2)O(2). Preliminary studies on the mechanisms suggest that H(2)O(2) withdraws electrons from the SWNT valence band by charge transfer, which suppresses the nanotube spectral intensity. The findings suggest possible enzyme-assisted molecular recognition applications by selective optical detection of biological species whose enzyme-catalyzed products include hydrogen peroxide.