铜（Ⅱ）－4－（吡啶－2′－甲基）－1，4，7，10－四氮杂环十三烷－11，13－二酮－α－氨基酸（5－取代邻菲罗啉）三元配合物的稳定性研究

用ｐＨ法在２５．０±０．１℃，Ｉ＝０．１ｍｏｌ·ｄｍ－３ＫＮＯ３条件下，测定铜（Ⅱ）－４－（吡啶－２′－甲基）－１，４，７，１０－四氮杂环十三烷－１１，１３－二酮－α－氨基酸（５－取代邻菲罗啉）三元配合物的稳定常数；比较了带吡啶甲基二氧四胺大环配体和二氧四胺大环配体与Ｃｕ（Ⅱ）的配位能力、配位方式；研究了该三元体系配合物稳定性与配体α－氨基酸酸碱强度之间的直线自由能关系；探讨了Ｃｕ（Ⅱ）和５－取代邻菲罗啉之间的ｄ－ｐ反馈π键以及邻菲罗啉取代基Ｈａｍｍｅｔｔ诱导效应与该三元配合物稳定性之间的关系。讨论了４－（吡啶－２′－甲基）－１，４，７，１０－四氮杂环十三烷－１１，１３－二酮和４－（喹啉－２′－甲基）－１，４，７，１０－四氮杂环十三烷－１１，１３－二酮与Ｃｕｘｐｈｅｎ所形成配合物的不同空间结构及其对配合物稳定性产生的影响。     

有机金属络合物用于高分子富氧膜的研究进展

本文介绍了能够可逆吸附分子氧的有机金属络合物用于高分子富氧膜的研究进展，以及以双重吸附理论进行的膜的促进输送机理。     

镧对镉胁迫下豌豆幼苗根部细胞内离子平衡及膜质子泵的影响

以水培豌豆(PisumsativumL)幼苗为材料,研究了La3+对Cd2+胁迫下豌豆幼苗生长、叶部与根部细胞对Cd2+的吸收、根部细胞内无机离子平衡体系及膜H+-ATPase,H+-PPase活性的影响。结果表明:Cd2+胁迫下喷施适宜浓度的La3+(0.50mg·L-1)可使豌豆幼苗相对生物量、苗长、叶绿素含量以及光合速率受到的毒害明显缓解,有效减少叶部与根部细胞对Cd2+的吸收,显著减轻了Cd2+胁迫导致的离子平衡系统的紊乱,明显缓解Cd2+对质膜及液泡膜H+-ATPase,H+-PPase活性的抑制作用。10mg·L-1Cd2+胁迫下La3+的保护效应比30mg·L-1Cd2+胁迫下更为显著。样品中La3+的测定和分析结果还表明,外源La3+处理后,La3+主要结合于细胞膜外,进入原生质体的仅占很小部分,但30mg·L-1Cd2+处理样品中La3+进入细胞的量则明显多于10mg·L-1Cd2+处理的样品。     

Studies on the Spectral Behavior between Protein with Poniacyl Carmine 2B and its Analytical Application

In this paper, the spectral behavior of protein and Poniacyl Carmine 2B (PC 2B) has been studied by spectrophotometric method. The conditional constants, apparent combination constant K and maximum binding number n, were used to express the combination ability of the reactions between PC 2B and protein under a set of given conditions. The Sandell index s was used to express the sensitivity of the determination of protein. The factors, acidity, PC 2B concentration and the ionic strength, were discussed by the change of apparent combination constant and maximum binding number. It was found that acidity of the solution, PC 2B concentration and ionic strength had a significant effect on the sensitivity of the assay of protein. Under the optimal conditions, the apparent combination constant K and the maximum binding number n were 2.36 × 10⁶ L mol^?1 and 95, respectively. With further investigation, it was found that the Scatchard model was suitable in treating the data obtained in the experiments. In the buffer medium of HCl‐KCl at 1.87, the addition of protein made the maximum absorption of the system move from 527 nm to 513 nm. Its apparent molar absorptivity is 4.46 × 10⁵ Lmol^?1 cm^?1 at 513 nm. Beer's law is obeyed in the range of 0 ? 55 μg mL^?1. The system developed in this paper has been used for the determination of protein in milk powder successfully.     

Fabrication of ultrathin films with large third-order nonlinear optical properties.

An ultrathin composite film containing anionic Ag-His complexes (His: L-Histidine) and oppositely charged BH-PPV was fabricated by means of the electrostatic layer-by-layer self-assembly technique. UV/Vis spectra showed a continuous deposition process of Ag-His complexes and BH-PPV. The film structure was characterized by using small-angle X-ray diffraction, AFM, and SEM. The nonlinear optical properties of the ultrathin film were studied by using the Z-scan technique with a laser duration of 8 ns at a wavelength of 532 nm. The film sample exhibited a strong nonlinear saturated absorption, with an alpha2 value of -3.9 x 10(-5) mW(-1) and a self-defocusing effect with an n2 value of -4.78 x 10 (-12) m2W(-1).     

Membrane ion-exchange chromatography for process-scale antibody purification

Microemulsion electrokinetic chromatography (MEEKC) was evaluated as a screening tool for the indirect measurement of octanol–water partition coefficients (log P_o/w) of pesticide compounds. Over 80 pesticide compounds representing a variety of structural characteristics were studied, and good correlation of log P_o/w with the logarithm of the retention factor was found. The microemulsion system studied allowed the separation of compounds in the log P_o/w range of −1 to 7. In addition, a smaller set of simple organic molecules that vary in structural features was evaluated and compared to the pesticide log P_o/w calibration. The pesticide and simple organic molecule log P_o/w calibration lines were statistically similar. This suggests that a universal set of standard compounds may be employed for the log P_o/w calibration to provide measurements for a variety of compounds with good accuracy.     

New fluorescent probes for the detection of mixed sodium and potassium metal ions

A new fluorescent probe 2 is developed that is capable of measuring the concentration of a mixture of sodium and potassium ions in the solution. This probe contains a fluorophore that is utilized in two ways depending on the pH of the solution.