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11.
Thorbjrn Vincent Snderby Zahra Najarzadeh Daniel Erik Otzen 《Molecules (Basel, Switzerland)》2022,27(13)
Functional amyloid is produced by many organisms but is particularly well understood in bacteria, where proteins such as CsgA (E. coli) and FapC (Pseudomonas) are assembled as functional bacterial amyloid (FuBA) on the cell surface in a carefully optimized process. Besides a host of helper proteins, FuBA formation is aided by multiple imperfect repeats which stabilize amyloid and streamline the aggregation mechanism to a fast-track assembly dominated by primary nucleation. These repeats, which are found in variable numbers in Pseudomonas, are most likely the structural core of the fibrils, though we still lack experimental data to determine whether the repeats give rise to β-helix structures via stacked β-hairpins (highly likely for CsgA) or more complicated arrangements (possibly the case for FapC). The response of FuBA fibrillation to denaturants suggests that nucleation and elongation involve equal amounts of folding, but protein chaperones preferentially target nucleation for effective inhibition. Smart peptides can be designed based on these imperfect repeats and modified with various flanking sequences to divert aggregation to less stable structures, leading to a reduction in biofilm formation. Small molecules such as EGCG can also divert FuBA to less organized structures, such as partially-folded oligomeric species, with the same detrimental effect on biofilm. Finally, the strong tendency of FuBA to self-assemble can lead to the formation of very regular two-dimensional amyloid films on structured surfaces such as graphite, which strongly implies future use in biosensors or other nanobiomaterials. In summary, the properties of functional amyloid are a much-needed corrective to the unfortunate association of amyloid with neurodegenerative disease and a testimony to nature’s ability to get the best out of a protein fold. 相似文献
12.
Leila Saedi Zahra Javanshir Salah Khanahmadzadeh Maryam Maskanati Milad Nouraliei 《Molecular physics》2020,118(7)
ABSTRACTDensity functional theory calculations were used to investigate the potential application of an AlN nanocluster in the detection of H2S, COS, CS2 and SO2 gases. In overall, the order of strength of interaction of these gases with the nanocluster is as follows: SO2 (Ead?=??17.6?kcal/mol)?>?H2S (Ead?=??14.0?kcal/mol)?>?COS (Ead?=??8.4?kcal/mol)?>?CS2 (Ead?=??4.5?kcal/mol). This indicates that by increasing the electric dipole moment, the adsorption energy becomes more negative. We found that the Al12N12 nanocluster may be a promising work function-type sensor for SO2 gas among the studied gases. Also, it is an electronic sensor for both SO2 and CS2 gases but selectively acts between them because of their different effects on the electrical conductivity. It is neither work function-type nor electronic sensor for H2S and COS gases. The AlN nanocluster benefits from a short recovery time about 7.7?s and 18.0?ms for desorption of SO2 and CS2 gases from its surface at room temperature, respectively. It is also concluded that this cluster can work at a humid environment. 相似文献
13.
Nitrogen doped nano porous graphene as a sorbent for separation and preconcentration trace amounts of Pb,Cd and Cr by Ultrasonic assisted in‐syringe dispersive micro solid phase extraction 下载免费PDF全文
Nitrogen doped nano porous graphene was used as an efficient sorbent in solid‐phase extraction process for simultaneous separation and pre‐concentration of metal ions lead (II), cadmium(II), and chromium(III)) in biological samples. Ultrasonic assisted in‐syringe dispersive micro solid phase extraction coupled with micro sampling atomic absorption spectrometry was utilized for the determination of metal ions. Nitrogen doped nano porous graphene was synthesized as a nano sorbent by chemical vapour deposition method. Methane and aniline were used as carbon and nitrogen sources. The characterization of sorbent was performed by field emission scanning electron microscope, transmission electron microscopy, atomic force microscope, fourier transform infrared, chemical element analysis and raman analysis. Effective parameters on the extraction efficiency such as pH, sorbent dosage, eluent volume and eluent concentration were optimized by central composite design and desirability function. Experimental results indicate that the optimal conditions for this extraction were pH = 6.4, 1.42 mg of sorbent, 100 μL of eluent, and 0.84 mol L‐1 of eluent concentration. The detection limits are as low as 1.5, 0.3 and 0.9 μg L‐1 for lead, cadmium, and chromium, respectively. The intra‐day precisions were 3.6, 4.38 and 2.94 and Inter‐day precision were 4.83, 5.26 and 4.52 for lead, cadmium, and chromium, respectively. Method performance was investigated by determination of mentioned heavy metals in complicated biological matrixes such as human plasma, urine and saliva samples with good recoveries. 相似文献
14.
Microchimica Acta - Graphene oxide nanosheets were modified with magnetite nanoparticles, and a hyperbranched polyamidoamine dendrimer was then covalently attached to their surface. The resulting... 相似文献
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16.
Mohammad Reza Islami Zahra Hassani Hassan Sheibani 《Phosphorus, sulfur, and silicon and the related elements》2013,188(11):2547-2554
Phthalimide or succinimide undergo a reaction with dialkyl acetylenedicarboxylates and triphenylphosphine to produce highly-functionalized stable heterocyclic phosphorus ylides in good yields in water-tetrahydrofuran (6:1). This methodology is of interest because of the use of water as solvent, thus minimizing the cost, operational hazards, and environmental pollution. 相似文献
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Fe3O4 magnetic nanoparticles functionalized with 5,10‐dihydropyrido[2,3‐b]quinoxaline‐7,8‐diol were synthesized as was their complex with copper as a novel nanomagnetic iron oxide catalyst via a simple and green method, and characterized using various techniques. The capability of the catalyst was evaluated in the one‐pot three‐component synthesis of different tetrazoles, which showed very good results. Mild reaction conditions, good reusability and simple magnetic work‐up make this methodology interesting for the efficient synthesis of tetrazoles. 相似文献
19.
Podophyllotoxin extraction from Linum usitatissimum plant and its anticancer activity against HT‐29, A‐549 and MDA‐MB‐231 cell lines with and without the presence of gold nanoparticles 下载免费PDF全文
Mohammad Safarpoor Mehrorang Ghaedi Masoume Yousefinejad Hamedreza Javadian Arash Asfaram Zahra Ghasemi Hajar Jaberi Daruosh Rahimi 《应用有机金属化学》2018,32(2)
In recent years, gold nanoparticles (Au‐NPs) have been taken into consideration in nanomedicine due to their excellent biocompatibility, chemical stability and promising optical properties. In this research, podophyllotoxin conjugated with gold nanoparticles (Au‐NPs‐POT) was synthesized and the conjugation of POT with Au‐NPs was confirmed using scanning electron microscopy, mass spectrometry and Fourier transform infrared spectroscopy. The anticancer effects of the product on preclinical models of lung, colon and breast cancers were investigated using MTT test. The analyses showed a direct dose–response relationship. It was found that higher concentrations of POT have more positive effects on the inhibition of cancer cell growth. At POT concentrations of 1, 2.5, 5, 7.5, 10, 15 and 20 ng ml?1, approximately 50% of the growth of colorectal, lung and breast cancer cell lines was inhibited, while similar results were obtained in the presence of 1, 2, 3, 4 and 5 μg ml?1 Au‐NPs‐POT. Au‐NPs‐POT exhibited the lowest cytotoxicity due to the presence of POT. The anticancer feature of Au‐NPs‐POT proved the potential to develop better anticancer therapeutics and to open new avenues for treatment of cancers. 相似文献
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