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101.
The high-sensitivity determination of protein concentrations by the enhancement of Rayleigh light scattering of Arsenazo-DBN 总被引:1,自引:0,他引:1
A new Rayleigh light scattering (RLS) assay of protein is presented in this paper. At the optimum pH 4.10, the weak RLS of Arsenazo-DBN can be greatly enhanced by the addition of proteins due to the interaction between protein and Arsenazo-DBN. Based on this, the reactions of Arsenazo-DBN and proteins, including bovine serum albumin, human serum album, gamma-globulin, egg albumin, lysozyme and trypsin, were studied. A new quantitative determination method for proteins has been developed. The linear range for human serum albumin, for example, is 0.085-34.62 micrograms mL-1 with a detection limit of 44.8 ng mL-1. Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good stability, and few interfering substances. The determination of the proteins in human serum and urine samples by this method give results very close to those obtained using Coomassie Brilliant Blue G-250 colorimetry, with relative standard deviations of 0.7-2.5%. 相似文献
102.
Density functional theory (DFT) and Monte Carlo (MC) simulation with free energy perturbation (FEP) techniques have been used to study the tautomeric proton transfer reaction of 2-amino-2-oxazoline, 2-amino-2-thiazoline, and 2-amino-2-imidazoline in the gas phase and in water. Two reaction pathways were considered: the direct and water-assisted transfers. The optimized structures and thermodynamic properties of stationary points for the title reaction system in the gas phase were calculated at the B3LYP/6-311+G(d, p) level of theory. The potential energy profiles along the minimum energy path in the gas phase and in water were obtained. The study of the solvent effect of water on the proton transfer of 2-amino-2-oxozoline, 2-amino-2-thiazoline, and 2-amino-2-imidazoline indicates that water as a solvent is favorable for the water-assisted process and slows down the rate of the direct transfer pathway. 相似文献
103.
104.
By using molecular dynamics simulation, the dynamic behaviors of particle permeation through a four-helix-bundle model channel are studied. The interior cavity of the four-helix-bundle provides the "routes" for particle permeation. The main structural properties of the model channel are similar to those that appear in natural four-helix-bundle proteins. It is found that the interior structure of the model channel may greatly influence the permeation process. At the narrow necks of the model channel, the particle would be trapped during the permeation. There is a threshold value for the driving force. When the driving force is larger than this threshold value, the mean first permeation time decreases sharply and tends to be saturated. Increasing the temperature of either the model channel or the particle reservoir can also facilitate the permeation. Enhancing the interaction strength between the particle and monomer on the four-helix-bundle model chain will hinder the permeation. Hence, the electrical current which is induced by the particle permeation is a function of the driving force and temperature. It is found that this current increases monotonically as the strength of the driving force or the temperature increases, but decreases as the interaction strength between the particle and monomer increases. It is also found that the larger the friction coefficient, the slower the permeation is. In addition, the multiparticle (or multi-ion) permeation process is also studied. The permeation of multiparticle is usually quicker than that of the single particle. The permeation of particle through a five-helix-bundle shows similar properties as that through a four-helix-bundle. 相似文献
105.
A sol-gel chemistry-based polymer coating approach was developed for the preparation of a novel polysiloxane-coated silica stationary phase for capillary liquid chromatography. SE-30, a commercial polysiloxane stationary phase used in gas chromatography, was incorporated into the properly designed sol solution. Then the sol-gel mixture was introduced into a silica gel-packed capillary column by pressure. A thin film of sol-gel SE-30-coating is chemically bonded to the surface of silica gel particles by hydrolytic polycondensation under mild conditions without any free radical cross-linking procedures, therefore the sol-gel approach offers a simple and effective pathway to create a hybrid polymer-coated silica stationary phase. Various factors affecting column making were optimized and discussed in this report. The resulting stationary phase showed good permeability, mechanical robustness, high durability to alkaline mobile phase and satisfactory chromatographic performance in separations of polar and non-polar aromatic compounds. Linear solvation energy relationships (LSERs) studies indicate that the stationary phase has a reversed-phased character with SE-30 providing chromatographic functionality. The solute size and the solute hydrogen bond ability are major factors that principally govern the retention of test solutes. 相似文献
106.
色谱柱是色谱分离分析的“心脏”,液相色谱技术的每一次重大进展都与分离固定相的突破密切相关。如上世纪70年代末期高效液相色谱技术的建立和90年代初期“灌流色谱”(Perfusion Chromatography)的发展都是基于多孔硅胶和“穿透孔”分离固定相的发展。近年来,基于特殊孔结构的1.5~2.0μm高强度复合材料的制备成功地催生了超高效液相色谱(UPLC)分离技术,而整体柱材料作为新一代的分离介质,已成为色谱领域广泛研究的前沿课题之一,并已经在样品预处理、手性分离、生物分离分析等领域获得十分广泛的应用。我国色谱研究工作者在多孔硅胶固定相、手性分离固定相、亲和色谱固定相和整体柱固定相等研究领域都取得了重大的进展,有些方面的研究工作已达到或领先于国际先进水平。 相似文献
107.
丙烯酸酯和甲基丙烯酸酯基团转移共聚研究 总被引:1,自引:0,他引:1
研究了三种丙烯酸酯分别和四种甲基丙烯酸酯的基团转移共聚,用1H NMR法测定共聚物组成,扩展的Kelen Tudos法测定竞聚率,结果为γMA=923、γMMA=006;γEA=1415、γMMA=001;γBA=751、γMMA=002;γMA=1441、γEMA=001;γMA=1396、γBMA=023;γMA=866、γi BMA=008,表明基团转移聚合同阴离子聚合有明显的相似之处. 相似文献
108.
从两二酸二乙酯、氯乙酸乙酯和氯苄出发,经LiAlH4还原,合成新的二元醇,进而合成新的螺环化合物:3,9-二羟乙基-3',9'-二苯甲基-1,5,7,11-四氧杂螺[5,5]十一烷.该化合物的结构由它的氢和碳-13核磁共振谱、红外光谱和元素分析得到证明.该单体在三氟化硼乙醚络合物的作用下进行开环聚合反应.由于环上四个取代基的稳定作用,聚合物的收率比较低(产率10%左右).通过对聚合物的结构分析,提出了这一单体的阳离子开环聚合反应机理.对随聚合温度升高,聚合物中酯基/苯基的克分子比降低,以及本体聚合时得到交联聚合物等一系列现象作了探讨. 相似文献
109.
Guoping Xue Jerald S. Bradshaw N. Kent Dalley Paul B. Savage Reed M. Izatt 《Journal of heterocyclic chemistry》2003,40(2):383-387
Two new trans‐disubstituted cyclam ligands; 1,8‐di(6‐hydroxymethylpyridin‐2‐ylmethyl)‐1,4,8,11‐tetra‐azacyclotetradecane ( 5 ) and 1,8‐dimethyl‐4, 11‐di(6‐hydroxymethylpyridin‐2‐ylmethyl)‐1,4,8,11 ‐tetraaza‐cyclotetradecane ( 6 ); have been synthesized and characterized. The crystal structures of ligand 6 and its Ni(II) and Co(II) complexes have been determined. Crystal data are given for 6 , space group, P21/c, a = 11.095 (6) Å, b = 9.467 (5) Å, c = 13.283 (8) Å; β = 106.95 (5)°, Z = 2, R = 0.0715; for [Ni 6 ](C104)2, space group P21/c, a = 9.4848 (14) Å, b = 33.941(6) Å, c = 9.793(2) A, β = 95.264(14)°, Z = 4, R = 0.0567; for [Co 6 ](C104)2, space group, P21/c, a = 9.440 (6) Å, b = 33.848 (13) Å, c = 9.820 (3) Å, β = 95.16(3)°, Z = 4, R = 0.0718. In both complexes, the metal atoms are six‐coordinate with only one of the pendants interacting with the central metal atom and the other pendant remaining uncoordinated. 相似文献
110.
YAN Zi-Feng 《天然气化学杂志》1996,5(2):116-125
1yttroductionIMcthancactivationisoncofthemostintriguingsubjectsinheterogencouscatal}'sisbccauscmcthancisathcrmod}namicall}'stabIccompoundt`ithanoblcgas-likeconfiguration.0fintcrcstsisthcrcccntt"orkreportcdb}'KocrtsIll,BeIguedl21.andYanl3ltthosucccssfull}'convcrtnaturalgasintohighcrh}.drocarbonsb}'thctt"o-stcproutcinwhichnaturalgasisfirstthcrmall}'activatcdonthctransitionmctalcatal}.stsatmoderatctemperature.Toasccrtainthcnaturcofrcactionsofmcthane.thcactivationofmcthaneisextensivel)'invcstiga… 相似文献