Time-resolved chemiluminescence technique for the microdetermination of proteins based on their complexation with copper(II).

Based on the complexation between proteins and Cu(II) coupled with the time-resolved chemiluminescence (CL) technique, a highly sensitive and quantitative assay for measuring proteins in solution is described. The complexes of proteins with Cu(II) have a strongly catalytic effect on the luminol-H2O2 CL reaction. Because the CL emission produced by the complexes is much more long-lived than that by Cu(II), the CL signals originating from proteins can be easily identified and measured with a time-resolved technique. On this basis, bovine albumin fraction V (BAF V) can be quantitatively determined in the range of 0.01 - 5.0 microg/ml with a detection limit of 5.8 ng/ml. The results show that the proposed assay exhibits a small variation in the response values for the same amount of different proteins, as compared to the Lowry as well as Bradford assays. The CL assay has also been studied for the detection of immobilized proteins.     

利用声波控制超短脉冲的色散

 声光互作用器件声光可编程色散滤波器（AOPDF）是一种实时的色散补偿器件，它可用于飞秒啁啾脉冲放大系统。通过耦合模理论推导，得到了输出脉冲的群延迟与调制声波的频率、啁啾、带宽等参数的关系，并且对用2.5cm长的LiNbO₃作为声光晶体的AOPDF补偿飞秒光脉冲的色散进行了数值模拟，得到了对中心波长为800 mm的钛宝石飞秒激光色散补偿特性及所需声波的频率和啁啾参数。     

提高三维激光扫描系统图像质量技术的研究

对其中三维激光扫描图像处理的关键技术———二值化、细化以及离散数据点云的滤波进行了研究,提出了一种先加权中值滤波,然后再小波分析滤波的新的降噪方法,并通过遗传算法对权重值进行了优化。在通常情况下,由于小波分析计算工作量大,所以提出了一种相对简单的算法,实现了在单台PC机上的运算。     

亚像元动态成像技术中系统调制传递函数的数值分析

亚像元动态成像技术是目前实现遥感器小型化非常有效的新方法,因而研究亚像元动态成像系统的调制传递函数MTF显得非常必要和重要.介绍了亚像元动态成像的基本原理及成像过程,指出了光程差对亚像元动态成像系统MTF的影响.对亚像元动态成像系统的MTF进行了数值分析,并指出了亚像元动态成像系统的成像质量及空间分辨率与一般成像系统相比较的优点.     

超声速等离子体射流的数值模拟

基于可压缩的全Naiver-Stokes方程，利用PHOENICS程序对由会聚 辐射阳极形状等离子体炬产生的超声速等离子体射流进行了数值模拟.考虑了等离子体的黏性、可压缩性以及变物性对等离子体射流特性影响.研究了超声速等离子体射流的流场结构特性以及不同环境压力对等离子体射流产生激波结构的影响.结果表明，超声速等离子体射流在喷口附近形成的周期性激波结构是其和环境气体相互作用的结果. 关键词： 等离子体炬 超声速等离子体射流 PHOENICS     

磁性Salisbury屏的高频响应

研究了利用磁性薄膜构造Salisbury屏的可能性及其在微波频段的反射率频率特性.结果表明，利用铁磁性材料在铁磁共振频率附近磁化率具有χ″>χ′的特性，可以构造出对电磁波有良好吸收性能的磁性Salisbury屏.通过对铁磁材料高频磁谱物理机理的分析后指出，具有弛豫型共振磁谱的铁磁材料可以构造出薄膜型Salisbury屏，其厚度为微米甚至亚微米量级.反射率的频率特性与磁性材料的特征阻抗z-r有关，它取决于铁磁共振频率和静态磁化率.反射率的频率响应显示磁性薄膜Salisbury屏具有较宽的吸收带宽. 关键词： 磁性Salisbury屏 反射率 频带响应 磁性薄膜     

双阳极磁控注入枪束流特性的研究

 介绍了用于34GHz基波回旋速调管的双阳极磁控注入枪的结构特点，为了准确分析磁控注入枪的束流特性，建立了阴极表面理论模型，用新编制的程序模拟了电子轨迹。模拟和测量结果显示磁场对磁控注入枪的束流有影响，磁控注入枪的束流也与阴极温度和空间电荷效应有关系。     

A mild and simple synthesis of N-aryl substituted toluenesulfamides under solvent-free conditions

N- aryl substituted benzenesulfamides are often used as heating-sensitive recording material1, thermal printing material2, sensitizer3 and developer4. Moreover, some of the benzenesulfamides have antifungal activities5. Many methods have been described for preparation of sulfamides. They are used to carry out in solvent8 or in solid phase condition9. These methods required solvent or solid support and even required heating or cooling. At the same time, the process of these methods is complex. Now we have developed a new method to prepare N-aryl substituted toluenesulfamides under solvent-free conditions.In recent years, solvent-free technology has gained popularity in organic synthesis. For instance,solidstate reaction and microwave reaction have received considerable attention. Solvent-free synthesis of amides has been reported10-11. This technology has many advantages such as high efficiency and selectivity, easy separation and environmental acceptability. All these merits are in accord with green chemistry's requirements of energy-saving, high efficiency and environmental benefits.In our paper, we used a simple and efficient method for preparing N-aryl substituted toluenesulfamides under solvent-free conditions, as a replacement for classic solvent, which gives many environmental benefits.All reactions were completed at room temperature by co-grinding in an agate mortar for 3-20min and the results are shown in Table 1.In conclusion, we have developed an efficient and convenient method of preparation N-aryl substituted toluenesulfamides in high yields. It symbols an improvement for synthesis of benzenesulfamides.     

PURIFICATION OF L-METHIONINE AND N-ACETYL-D-METHIONINE FROM THE MIXTURE OF ENZYMATICALLY DEACYLATED N-ACETYL-DL-METHIONINE

1. INTRODUCTION Methionine, namely 2-amido-4-thiomethyl butyric acid with a structure of CH3SCH2CH2CHCOOH, is one of the essential amino acids and has two natural enantionmers, D and L-methionine. The mixture of L- and D-isomers can be used as analeptics or nutritive additives to maintain the equilibrium of amino acids of feed [1,2]. L-methionine can release active methyl and accelerate the synthesis of choline, which further speeds up the conversion of the lipid accumulated in liv…     

Three Novel Eremophilanolides from Ligularia virgaurea spp. oligocephala

From the alcoholic extract of the whole plant of Ligularia virgaurea spp. oligocephala, three novel eremophilane sesquiterpene lactones, 6α, 10α-dihydroxy- 1-oxoeremophila-7(11), 8(9)-dien-8, 12-olide, 613, 10α-dihydroxy-1-oxoeremophila-7 (ll), 8 (9)-dien-8, 12-olide and 10α-hydroxy-1-oxoeremophila-7(l 1), 8(9)-dien-8, 12-olide were isolated. Their structures were elucidated by various spectroscopic methods including intensive 2D NMR techniques (COSY,HMQC, HMBC and ^1H-^1H NOESY) and HR-MS.