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31.
32.
Inoue IH Watanabe M Kinoshita T Kakizaki A Kato R Kobayashi A Kobayashi H Fujimori A 《Physical review. B, Condensed matter》1993,47(19):12917-12920
33.
An automated high-performance liquid chromatographic method using alkali-ninhydrin reagent for the post-column derivatization of guanidines has been developed. This procedure was applied to measurements made before and after haemodialysis. Among the guanidino compounds found in human blood, methylguanidine showed the lowest removal rate. The removal rate of guanidinosuccinic acid correlated with the plasma alpha 1-globulin fraction. The removal rate of each guanidino compound decreased with the period of dialysis. 相似文献
34.
Harada S Kumagai N Kinoshita T Matsunaga S Shibasaki M 《Journal of the American Chemical Society》2003,125(9):2582-2590
Full details of our direct Michael addition of unmodified ketones using new asymmetric zinc catalysis are described. Et(2)Zn/(S,S)-linked-BINOL complexes were successfully applied to direct 1,4-addition reactions of hydroxyketones. The first generation Et(2)Zn/(S,S)-linked-BINOL 1 = 2/1 system was effective for 1,4-addition of 2-hydroxy-2'-methoxyacetophenone (3). Using 1 mol % of (S,S)-linked-BINOL 1 and 2 mol % of Et(2)Zn, we found that a 1,4-addition reaction of beta-unsubstituted enone proceeded smoothly at 4 degrees C to afford products in high yield (up to 90%) and enantiomeric excess (up to 95%). In the case of beta-substituted enones, however, the first generation Et(2)Zn/(S,S)-linked-BINOL 1 = 2/1 system was not at all effective. The second generation Et(2)Zn/(S,S)-linked-BINOL 1 = 4/1 with MS 3A system was developed and was effective for various beta-substituted enones to afford products in good dr, yield (up to 99%), and high enantiomeric excess (up to 99% ee). With the Et(2)Zn/1 = 4/1 systems, catalyst loading for beta-unsubstituted enone was reduced to as little as 0.01 mol % (substrate/chiral ligand = 10 000). The new system was also effective for 1,4-addition reactions of 2-hydroxy-2'-methoxypropiophenone (9) to afford chiral tert-alcohol in high enantiomeric excess (up to 96% ee). Mechanistic investigations as well as transformations of the Michael adducts into synthetically versatile intermediates are also described. 相似文献
35.
Kinoshita M Murakami E Oda Y Funakubo T Kawakami D Kakehi K Kawasaki N Morimoto K Hayakawa T 《Journal of chromatography. A》2000,890(2):261-271
Frontal affinity chromatography is a method for quantitative analysis of biomolecular interactions. We reinforced it by incorporating various merits of a contemporary liquid chromatography system. As a model study, the interaction between an immobilized Caenorhabditis elegans galectin (LEC-6) and fluorescently labeled oligosaccharides (pyridylaminated sugars) was analyzed. LEC-6 was coupled to N-hydroxysuccinimide-activated Sepharose 4 Fast Flow (100 μm diameter), and packed into a miniature column (e.g., 10×4.0 mm, 0.126 ml). Twelve pyridylaminated oligosaccharides were applied to the column through a 2-ml sample loop, and their elution patterns were monitored by fluorescence. The volume of the elution front (V) determined graphically for each sample was compared with that obtained in the presence of an excess amount of hapten saccharide, lactose (V0); and the dissociation constant, Kd, was calculated according to the literature [K. Kasai, Y. Oda, M. Nishikawa, S. Ishii, J. Chromatogr. 376 (1986) 33]. This system also proved to be useful for an inverse confirmation; that is, application of galectins to an immobilized glycan column (in the present case, asialofetuin was immobilized on Sepharose 4 Fast Flow), and the elution profiles were monitored by fluorescence based on tryptophan. The relative affinity of various galectins for asialofetuin could be easily compared in terms of the extent of retardation. The newly constructed system proved to be extremely versatile. It enabled rapid (analysis time 12 min/cycle) and sensitive (20 nM for pyridylaminated derivatives, and 1 μg/ml for protein) analyses of lectin–carbohydrate interactions. It should become a powerful tool for elucidation of biomolecular interactions, in particular for functional analysis of a large number of proteins that should be the essential issues of post-genome projects. 相似文献
36.
Kinoshita T 《Chemical & pharmaceutical bulletin》2000,48(9):1375-1377
New cassane diterpenes named neocaesalpins C and D were isolated from the Philippine crude drug calumbibit botanically originating from the seeds of Caesalpinia bonduc (Fabaceae), and their structures were elucidated on the basis of the spectroscopic evidence. These compounds are characterized by the presence of the alpha,beta-butenolide moiety. Although a number of cassane furanoditerpenes have been known to occur in the same plant species, such constituents could not be isolated from the crude drug of Philippine origin in this study. It is presumed that the chemical difference resulted from chemical differentiation of the species. 相似文献
37.
Koga T Taguchi K Kinoshita T Higuchi M 《Chemical communications (Cambridge, England)》2002,(3):242-243
A novel artificial protein with simple primary structure, poly(gamma-methyl-L-glutamate)-grafted polyallylamine, has been prepared and the resultant peptide has shown a unique property of pH-regulated conformation and morphology. 相似文献
38.
39.
Takuma Shinya Nakashima Noriyuki Tantirungkij Manee Kinoshita Shinichi Okada Hirosuke Sew TatsÜji Yoshida Toshiomi 《Applied biochemistry and biotechnology》1991,28(1):327-340
Applied Biochemistry and Biotechnology - A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast,Pichia stipitis. DNA sequence analysis showed that the gene... 相似文献
40.
Asymmetric membrane potentials and transport properties of polypeptide membrane consisting of two layers with poly(L-glutamic acid) and poly (γ-methylL-glutamate) were studied in the pH range of 2 to 6 at 25 °C. Under the condition adopted, the poly (L-glutamic acid) layer of the membrane underwentα helix to coil transition which was confirmed by ATR-IR measurements. The membrane potentials of the asymmetric membrane between two identical solutions of KCl,Δψ asym, which is called asymmetric potential, were observed.Δψ asym values were effected by the structural transition of poly (L-glutamic acid) layer. As a result, the effective charge density of the membrane, which was derived by the asymmetric membrane potential measurements, had a maximum at the transition region of pH=4.8. On the other hand, the permeation coefficient of KCl,P i, was higher in the direction from poly (L-glutamic acid) side to poly (γ-methylL-glutamate) side, “G →M direction”, than in the oppositeM →G direction. Furthermore,P i inG →M direction was dependent on the solution pH, that is,P i decreased when pH was increased to 4.8 and increased on further increasing of pH These membrane behaviors were described in terms of the competition between structural transition and variation of fixed charge density owing to the helix to coil transition of the asymmetric polypeptide membrane. 相似文献