Synthesis of Hydrotalcite from Seawater and Its Application to Phosphorus Removal

10.0 wt% milk of lime was added to seawater containing AlCl 3 at Mg/Al molar ratio of 3.0 until pH 10.5 with stirring, and kept at 25;C for 1 h. Hydrotalcite (HT) was precipitated as a single phase, and Mg 2+ and Al 3+ were quantitatively precipitated. The chemical composition was [Mg 0.75 Al 0.25 (OH) 2 ][(SO 4 ) 0.06 (Cl) 0.02 (OH) 0.11 *];0.27H 2 O* (*Balance). A 100 mg-P/L Na 2 HPO 4 solution and the HT were shaken at 25;C. Phosphate removal increased with increasing time and the HT quantity, and was the highest at pH 7-9. Phosphate ion could be quantitatively removed, adding 8 times the stoichiometric quantity of the HT at pH 8.7 for 6 h.     

Photoinduced Upregulation of Calcitonin Gene-Related Peptide in A549 Cells through HNO Release from a Hydrophilic Photocontrollable HNO Donor

Nitroxyl (HNO), a one-electron-reduced form of nitric oxide, has various biological activities, including a cardioprotective effect. Here, we first synthesized another, more hydrophilic photocontrollable HNO donor (3), which can release HNO in a spatially and temporally controlled manner, and then examined the properties of our series of compounds as practical HNO donors in a cellular system under photocontrol. We selected compound 2 as the preferred donor, and used it to show that calcitonin gene-related peptide (CGRP) can be upregulated in A549 cells via photocontrolled HNO release. This result demonstrates the suitability of this photocontrollable HNO donor for biological investigations.     

On-line concentration and fluorescence determination HPLC for polycyclic aromatic hydrocarbons in seawater samples and its application to Japan Sea

An on?line concentration and fluorescence determination HPLC for polycyclic aromatic hydrocarbons (PAHs) in seawater was proposed. An online concentration column packed with octadecyl polyvinyl alcohol polymer, a pump and a column switching valve were introduced in the conventional HPLC with a fluorescence detector. Only 1.0-100?mL seawater sample was introduced into the concentration column at 1.0?mL?min(-1) without any other pretreatment except filtration. Then the trapped PAHs totally flew into the separation column and eluted separately to be detected fluorogenically. The proposed method had good linearity with correlation coefficients (r) ranged from 0.951 to 0.998, and limits of detection ranged from 0.002 to 0.50?ng?L(-1) for 15 PAHs as 100?mL seawater was loaded. The sensitivity of the method was 10 to 100 times higher than those reported by other works. The proposed method was applied to the determination of PAHs in the seawater samples collected in the Japan Sea with satisfactory results and to check the present benzo[a]pyrene concentration at the beaches in Noto peninsula, Japan polluted with C-heavy oil spilled from the tanker in 1997.     

Preparation of Mg–Al layered double hydroxides intercalated with 1,3,6-naphthalenetrisulfonate and 3-amino-2,7-naphthalenedisulfonate and assessment of their selective uptake of aromatic compounds from aqueous solutions

Mg–Al layered double hydroxides (Mg–Al LDHs) intercalated with 1,3,6-naphthalenetrisulfonate (NTS^3?) and 3-amino-2,7-naphthalenedisulfonate (ANDS^2?) ions were prepared by coprecipitation and were characterized by X-ray diffraction and chemical analyses. Based on X-ray diffraction patterns, the naphthalene rings of NTS^3? and ANDS^2? were most likely oriented parallel to the brucite-like host layers of the Mg–Al LDH, midway between layers. The prepared Mg–Al LDHs were able to selectively take up aromatics from aqueous solutions, and the order of percentage uptake was as follows: 1,3-dinitrobenzene > nitrobenzene > benzaldehyde > N,N-dimethylaniline > anisole > 1,2-dimethoxybenzene. The differences in the extent of π–π stacking interactions occurring between the benzene rings of the aromatics and the naphthalene ring of the intercalated NTS^3? and ANDS^2? probably resulted in these differences among the absorbed quantities of the various aromatics.     

Is the 7/2(1)- isomer state of 43S spherical?

We report on the spectroscopic quadrupole moment measurement of the 7/2(1)(-) isomeric state in (16)(43)S(27) [E*=320.5(5) keV, T(1/2)=415(3) ns], using the time dependent perturbed angular distribution technique at the RIKEN RIBF facility. Our value, |Q(s)|=23(3) efm(2), is larger than that expected for a single-particle state. Shell model calculations using the modern SDPF-U interaction for this mass region reproduce remarkably well the measured |Q(s)|, and show that non-negligible correlations drive the isomeric state away from a purely spherical shape.     

Synthesis of 1-fluoroindan-1-carboxylic acid (FICA) and its properties as a chiral derivatizing agent

1-Fluoroindan-1-carboxylic acid (FICA) (1) was designed and synthesized as its methyl ester (FICA Me ester) (4) in order to develop an efficient chiral derivatizing agent (CDA) which excels α-methoxy-α-(trifluoromethyl)phenylacetic acid (MTPA) in capability. FICA Me ester (4) was prepared by fluorination of methyl 1-hydroxyindan-1-carboxylate (3) with (diethylamino)sulfur trifluoride (DAST) and derived to the esters of racemic secondary alcohols by ester exchange reaction. The resulting Δδ_F value was large in the case of 2-butyl ester of FICA (5a), whereas not detectable in the case of the corresponding MTPA ester (6a). The magnitude of the Δδ_H values was similar to that of MTPA esters. The diastereomers of (R)-(−)-8-phenylmenthyl ester of FICA (5i) was separated and their ¹H NMR analyses revealed that the concept of the modified Mosher's method was successfully applied to 5i.     

Intravenous administration of mesenchymal stem cells exerts therapeutic effects on parkinsonian model of rats: Focusing on neuroprotective effects of stromal cell-derived factor-1α

Background

Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability.

Results

So far, the expression pattern of JAM-A has not been described in detail for the different cell types of the adult brain. Here we show that a subset of proliferating cells in the adult mouse brain express JAM-A. We further clarify that these cells belong to the lineage of NG2-glia cells. Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution. Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.

Conclusions

Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.     

High-Energy X-ray Diffraction and MD Simulation Study on the Ion-Ion Interactions in 1-Ethyl-3-methylimidazolium Bis(fluorosulfonyl)amide

Ion-ion interactions or liquid structures in low-viscosity ionic liquid, 1-ethyl-3-methylimidazolium bis(fluorosulfonyl)amide, [C₂mIm⁺][FSA^?] were investigated by high-energy X-ray diffraction (HEXRD) experiments and molecular dynamics (MD) simulations. Experimental X-ray structure factor, S ^exp(q) obtained from the HEXRD was successfully deconvoluted into the intra- and the intermolecular components, S _intra ^exp (q) and S _inter ^exp (q), respectively, by taking into account the population of cis and trans conformers of the FSA anion to give the corresponding radial distribution functions, G _intra ^exp (r) and G _inter ^exp (r), respectively. The G _inter ^exp (r) exhibits the peaks at 3.5, 4.6 and 5.4 Å, which is well represented by theoretical radial distribution function, G _inter ^MD (r) obtained from MD simulations. From the space distribution function, SDF calculated by MD simulations, it was found that static structure (distance and orientation) of the nearest neighbor intermolecular interaction between cation and anion in [C₂mIm⁺][FSA^?] is similar to its analogous ionic liquid, [C₂mIm⁺][TFSA^?] where TFSA is bis(trifluoromethanesulfonyl)amide.