阴阳离子型表面活性剂双水相萃取色氨酸衍生物和牛血清白蛋白

溴化十二烷基三乙胺（Ｃ１２ＮＥ）和十二烷基硫酸钠（ＳＤＳ）在一定条件下混合可以形成具有清晰界面的两个水相，称为阴阳离子型表面活性剂双水相。作者研究了利用该双水相萃取３种色氨酸衍生物和牛血清白蛋白的可能性．文中采用工作曲线校正扣除表面活性剂背景的影响，萃取结果准确可信。     

A New β-Carboline Alkaloid and a New Derivate of Isoferulic Acid from Anemone altaica

A new β-carboline alkaloid, 4-（9H-β-carbolin-1-yl）-4-oxobutyric acid and a new derivate of isoferulic acid, （E）-3-（3-hydroxy-4-methoxyphenyl）acrylic acid carboxymethyl ester, were isolated from the roots of Anemone altaica. Their structures were determined on the basis of spectral data.     

Determination of binding sites in carboplatin-bound cytochrome c using electrospray ionization mass spectrometry and tandem mass spectrometry

Interaction of carboplatin with cytochrome c (Cyt. c) has been investigated by electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS). ESI-MS studies revealed that the ring-opened adducts of carboplatin with Cyt. c were formed in the stoichiometric ratio of 1:1 and 2:1 at pH 5.0 and 37 degrees C and in the stoichiometric ratio of 1:1 only at pH 7.0 and 37 degrees C. It was also found that Cyt. c could be cleaved by carboplatin at pH 2.5 and 50 degrees C. The cleaved fragments of Cyt. c were determined by ESI-MS and MS/MS analysis to be Glu66 approximately Met80, Ac-Gly01 approximately Met65, Glu66 approximately Glu104, Ac-Gly01 approximately Met80 and Ile81 approximately Glu104. The carboplatin prefers to anchor to Met65 first, then to Met80. To further confirm the binding site of Met, AcMet-Gly was used as the model molecule to investigate its interaction with carboplatin and its hydrolysis reaction. On the basis of species detected during the reaction monitored by ESI-MS, a possible pathway of the cleavage reaction was proposed.     

锌与小儿神经系统及代谢性疾病

综述了锌在小儿神经系统疾病和内分泌代谢性疾病中的作用及临床意义，尤其是对癫痫的发病起重要作用，大量资料证明高锌与癫痫发作有关，其血锌值明显高于健康儿童，因此在治疗中不要轻易补锌。还综述了锌与智能发育障碍、生长发育障碍、多动症、胎儿畸形、遗传性感音神经性聋等疾病的关系及在治疗糖尿病、肝豆状核变性等内分泌和代谢性疾病时要注意维持锌的正常动态平衡。     

Synthesis, Crystal Structure and Properties of [Co(1,3- bdoaH)_2·4H_2O]·4H_2O with Benzene-1,3-dioxyacetate Ligand

1INTRODUCTIONTheorganicaromaticcarboxylatemetalcom-plexeshavearousedmuchattentionandbeenwidelyinvestigated.However,thestrategyofdesignandsynthesisofthesecomplexesismostlyfocusedonrigidligands,suchasterephthalicacidandbenzenetricarboxylicacid,etc.[1～5].Theassemblyofcom-plexesbyusingflexiblearomaticcarboxylateli-gandsisfarlessdeveloped[6,.Phenylenedioxydia-7]ceticacids(bdoaH2)withbiologicalactivitiesandwideapplicationsinagriculture[8]areafamilyofmultidentateflexibleligandswithversatilebind…     

用丘氏气提定氮器测定植物中的氮

研究了用气提滴定法测定植物中氮的条件和方法．实验结果表明，与蒸馏法相比，方法简便安全，结果准确     

Electrospun nanofibers of poly（vinyl pyrrolidone）/Eu^3＋ and its photoluminescence properties

Nanofibers of poly（vinyl pyrrolidone） （PVP）/Eu^3＋ with diameters of 300-900 nm were prepared by using sol-gel processing and electrospinning technique. The products were characterized by scanning electron microscope （SEM）, Fourier transform infrared spectroscopy （FT-IR）, and photoluminescence （PL）. The results indicated that, Eu^3＋ was successfully embedded in the onedimensional hybrid nanofibers, and the PVP/Eu^3＋ hybrid nanofibers had favorable photoluminescence properties.     

Electron propagator theory study of N-/O-methylglycine conformers

The low-lying conformers of N-/O-methylglycine are studied by ab initio calculations at the B3LYP, MP3, and MP4(SDQ) levels of theory with the aug-cc-pVDZ basis set. The conformers having the intramolecular hydrogen bonds N-H...O=C or O-H...N are more stable than the others. Vertical ionization energies for the valence molecular orbitals of each conformer predicted with the electron propagator theory in the partial third-order quasiparticle approximation are in good agreement with the experimental data available in the literatures. The relative energies of the conformers and comparison between the simulated and the experimental photoelectron spectra demonstrate that there are at least three and two conformers of N- and O-methylglycine, respectively, in the gas-phase experiments. The intramolecular hydrogen bonding O-H...N effects on the molecular electronic structures are discussed for the glycine methyl derivatives, on the basis of the ab initio electronic structure calculations, natural orbital bond, and atoms-in-molecules analyses. The intramolecular hydrogen bonding O-H...N interactions hardly affect the electronic structures of the O-NH2-CH2-C(=O)-O-CH3 and alpha-methylated NH2-CH2-C(CH3)OOH conformers, while the similar intramolecular interactions lead to the significantly lower-energy levels of the highest occupied molecular orbitals for the N-(CH3-NH-CH2-COOH) and beta-methylated (NH2-CH2-CH2-COOH) conformers.     

The migrastatin family: discovery of potent cell migration inhibitors by chemical synthesis

The first asymmetric total synthesis of (+)-migrastatin (1), a macrolide natural product with anti-metastatic properties, has been accomplished. Our concise and flexible approach utilized a Lewis acid-catalyzed diene aldehyde condensation (LACDAC) to install the three contiguous stereocenters and the trisubstituted (Z)-alkene of migrastatin (2 + 3 --> 21). Construction of the two remaining stereocenters and incorporation of the glutarimide-containing side chain was achieved by an anti-selective aldol addition of propionyl oxazolidinone 28 to angelic aldehyde 27, followed by a Horner-Wadsworth-Emmons (HWE) coupling of 32 with glutarimide aldehyde 5. Finally, the assembly of the macrocycle was realized by a highly (E)-selective ring-closing metathesis (35 --> 37). Utilizing the power of diverted total synthesis (DTS), a series of otherwise inaccessible analogues was prepared and evaluated for their potential as tumor cell migration inhibitors in several in vitro assays. These studies revealed a dramatic increase in activity when the natural motif was considerably simplified, presenting macrolactones 45 and 48, as well as macrolactam 55, macroketone 60, and CF(3)-alcohol 71 as promising anti-metastatic agents.     

A theoretical study of the interactions between N, N-dimethylformamide and aromatic hydrocarbons

The B3LYP and MP2 methods with 6-31G* basis set were used to predict the geometries of N, N-dimethylformamide (DMF) dimer and DMF–aromatic hydrocarbons interaction systems. A total of 10 conformers were obtained with no imaginary frequencies, respectively. The interaction energies of these binary mixtures have been obtained. The analyses of chelpg charge distribution and the atoms in molecules theory (AIM) were used to analyze the nature of the interaction. The results show the presence of hydrogen bonds between DMF and aromatic hydrocarbons. The interaction between DMF and benzonitrile is the strongest with the interaction energy of −21.58 kJ mol⁻¹ (BSSE corrected), and the intensity order of interactions is DMF–benzonitrile: d2 > DMF–DMF: a2 > DMF–toluene: c1 > DMF–benzene: b2.