全文获取类型
收费全文 | 590篇 |
免费 | 20篇 |
专业分类
化学 | 509篇 |
晶体学 | 3篇 |
力学 | 8篇 |
数学 | 12篇 |
物理学 | 78篇 |
出版年
2023年 | 4篇 |
2022年 | 4篇 |
2021年 | 9篇 |
2020年 | 12篇 |
2019年 | 12篇 |
2018年 | 8篇 |
2017年 | 4篇 |
2016年 | 12篇 |
2015年 | 11篇 |
2014年 | 22篇 |
2013年 | 24篇 |
2012年 | 42篇 |
2011年 | 53篇 |
2010年 | 24篇 |
2009年 | 23篇 |
2008年 | 29篇 |
2007年 | 47篇 |
2006年 | 32篇 |
2005年 | 31篇 |
2004年 | 30篇 |
2003年 | 30篇 |
2002年 | 15篇 |
2001年 | 8篇 |
2000年 | 1篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1995年 | 1篇 |
1994年 | 5篇 |
1992年 | 6篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 8篇 |
1988年 | 8篇 |
1987年 | 12篇 |
1986年 | 11篇 |
1985年 | 15篇 |
1984年 | 8篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 8篇 |
1977年 | 1篇 |
1974年 | 2篇 |
1965年 | 1篇 |
1935年 | 2篇 |
排序方式: 共有610条查询结果,搜索用时 15 毫秒
601.
Miyashita M Hanai Y Awane H Yoshikawa T Miyagawa H 《Rapid communications in mass spectrometry : RCM》2011,25(9):1130-1140
An improved method of de novo peptide sequencing based on mass spectrometry using novel N-terminal derivatization reagents with high proton affinity has been developed. The introduction of a positively charged group into the N-terminal amino group of a peptide is known to enhance the relative intensity of b-ions in product ion spectra, allowing the easy interpretation of the spectra. However, the physicochemical properties of charge derivatization reagents required for efficient fragmentation remain unclear. In this study, we prepared several derivatization reagents with high proton affinity, which are thought to be appropriate for peptide fragmentation under low-energy collision-induced dissociation (CID) conditions, and examined their usefulness in de novo peptide sequencing. Comparison of the effects on fragmentation among three derivatization reagents having a guanidino or an amidino moiety, which differ in proton affinity, clearly indicated that there was an optimal proton affinity for efficient fragmentation of peptides. Among reagents tested in this study, derivatization with 4-amidinobenzoic acid brought about the most effective fragmentation. This derivatization approach will offer a novel de novo peptide sequencing method under low-energy CID conditions. 相似文献
602.
The activities of enkephalin-degrading peptidases such as enkephalinases A and B in rat brain tissues were simultaneously assayed by a high-performance liquid chromatographic method with fluorimetric detection with an automatic reaction system. Tyrosine and tyrosine-containing peptides produced enzymatically from the substrate, methionine-enkephaline, were separated by gradient elution on a reversed-phase column (TSK gel ODS-120T), and then converted into fluorescent derivatives for detection by reaction with hydroxylamine, cobalt(II) and borate reagents. The method permits the simple and sensitive detection of N-terminal tyrosine-containing fragments of the enkephalin peptide. The limits of detection are 5-20 pmol per assay tube for the N-terminal tyrosine-containing fragments. The enzyme activities in the regionally separated tissues were 54-191 pmol/min.mg protein for enkephalinase A and 79-153 pmol/min.mg protein for enkephalinase B, which were calculated from the formation of Tyr-Gly-Gly and Tyr-Gly, respectively, during the enzyme reaction. 相似文献
603.
Cycle is one of the most fundamental graph classes. For a given graph, it is interesting to find cycles of various lengths as subgraphs in the graph. The Cayley graph on the symmetric group has an important role for the study of Cayley graphs as interconnection networks. In this paper, we show that the Cayley graph generated by a transposition set is vertex-bipancyclic if and only if it is not the star graph. We also provide a necessary and sufficient condition for to be edge-bipancyclic. 相似文献
604.
High-performance liquid chromatographic determination of free and total polyamines in human serum as fluorescamine derivatives 总被引:1,自引:0,他引:1
A highly sensitive and simple fluorimetric method for the determination of free and total polyamines, spermidine, spermine, putrescine and cadaverine, in human serum by high-performance liquid chromatography is described. The polyamines, obtained after clean-up of deproteinized serum by Cellex P column chromatography, are converted to their fluorescamine derivatives in the presence of nickel ion which inhibits the reaction of interfering amines with fluorescamine, and the derivatives are separated simultaneously by reversed-phase chromatography (LiChrosorb RP-18) with a linear gradient elution. The lower limits of detection are 10 and 15 pmole for spermine and the others in 0.5 ml of serum, respectively. 相似文献
605.
Michael R. Harpham Son C. Nguyen Zongrui Hou Jeffrey C. Grossman Charles B. Harris Michael W. Mara Andrew B. Stickrath Yosuke Kanai Alexie M. Kolpak Donghwa Lee Di‐Jia Liu Justin P. Lomont Kasper Moth‐Poulsen Nikolai Vinokurov Lin X. Chen K. Peter C. Vollhardt 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2012,124(31):7812-7816
606.
607.
Abstract We have observed the quadratic laser power dependence of two anti-Stokes emission bands at 15100cm?1 and 14000cm?1 in an additively colored KCl when excited with a Nd:YAG laser. The cross section of two photon absorption to cause the 14000cm?1 band is estimated to be 4×10?40cm4sec. We have observed that emission intensities are dependent on temperature in the range between 4. 2K and 130K. They are enhanced by means of optical bleaching and also with the increase of the F concentration. From a qualitative analysis of these observations, we propose that these emissions are correlated to the loose aggregation of the F centers. 相似文献
608.
Abstract The resonance Raman scattering (RRS) of F centers, particularly the photon-energy dependence of linear polarization of the RRS, has been studied on the basis of a vibronic viewpoint. With combinination of previous results that have explained magnetic circular dichroism (MCD) spectra, a vibronic scheme of the Franck-Condon state of F centers for KCl, KBr and KI crystals is established. 相似文献
609.
Sotaro Inomata Yusuke Harada Yuya Nakamura Yosuke Uehara Takashi Ishizone 《Journal of polymer science. Part A, Polymer chemistry》2013,51(19):4111-4124
Cationic ring‐opening polymerizations of 5‐alkyl‐ or 5,7‐dialkyl‐1,3‐dehydroadamantanes, such as 5‐hexyl‐ ( 4 ), 5‐octyl‐ ( 5 ), 5‐butyl‐7‐isobutyl‐ ( 6 ), 5‐ethyl‐7‐hexyl‐ ( 7 ), and 5‐butyl‐7‐hexyl‐1,3‐dehydroadamantane ( 8 ), were carried out with super Brønsted acids, such as trifluoromethanesulfonic acid or trifluoromethanesulfonimide in CH2Cl2 or n‐heptane. The ring‐opening polymerizations of inverted carbon–carbon bonds in 4–8 proceeded to afford corresponding poly(1,3‐adamantane)s in good to quantitative yields. Poly( 4–8 )s possessing alkyl substituents were soluble in 1,2‐dichlorobenzene, although a nonsubstituted poly(1,3‐adamantane) was not soluble in any organic solvent. In particular, poly( 8 ) exhibited the highest molecular weight at around 7500 g mol?1 and showed excellent solubility in common organic solvents, such as THF, CHCl3, benzene, and hexane. The resulting poly( 4–8 )s containing adamantane‐1,3‐diyl linkages showed good thermal stability, and 10% weight loss temperatures (T10) were observed over 400 °C. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013 , 51, 4111–4124 相似文献
610.
Yuushou Nakayama Takamichi Inaba Yosuke Toda Ryo Tanaka Zhengguo Cai Takeshi Shiono Hiroyuki Shirahama Chikara Tsutsumi 《Journal of polymer science. Part A, Polymer chemistry》2013,51(20):4423-4428
l ‐Lactide (l ‐LA) was polymerized in the presence of N‐methyldiethanolamine as an initiator and Sn(Oct)2 as a catalyst to give hydroxy‐telechelic poly(l ‐lactide) (PLLA‐diol) bearing a tertiary amine group at the center of the polymer chain. Successive chain extension of the PLLA‐diol with hexamethylene diisocyanate afforded PLLA‐based poly(ester‐urethane)s (PEU) with equally spaced tertiary amine groups. Treatment of the PEU with iodomethane converted tertiary amine groups to quaternary ammonium groups to give cationic ionomers (PEU‐MeI). The thermal, mechanical, hydrophilic, and biodegradation properties of the obtained polymers were investigated. The thermal properties of the PEUs and the PEU‐MeIs were similar each other. The PEU‐MeIs exhibited higher tensile modulus than those of the starting PEUs. The contact angles of water on the PEU‐MeIs were lower than those of the PEUs with similar NMDA content indicating their higher hydrophilicity. In compost degradation tests, the PEU‐MeIs showed slower degradation than those of the PEUs. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013, 51, 4423–4428 相似文献