A Highly Sensitive Method to Identify the D,L-Configurations of Monosaccharides Based on (-)-TBMB Carboxylic Acid and HPLC

Abstract

D, L-Isomers of monosaccharides were coupled with a fluorescent chiral derivatizing agent, (-)-2-tert-butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid [(-)-TBMB carboxylic acid], to afford a pair of diastereoisomers. The two were well separated with HPLC (reverse phase conditions; ODS, CH₃CN:H₂O:isoPrOH) within 60 min and could be detected by fluorescence of the TBMB group at the few picomolar level.     

High Accuracy Phase Measurement in Phase-Shifting Speckle Interferometry

A method for accurately measuring information about the deformation of a rough surface object using a phase-shifting speckle interferometer with a television camera and a computer is considered. In this case, the intensity change of the speckle by phase-shifting varies randomly in space because of the statistical property of the speckle. Then, at points with small intensity change, the accuracy of the phase measurement is affected significantly by the quantization error of an analog to digital converter for data recording. To improve the accuracy, the statistical property of the interference speckle must be clarified. This is done theoretically and experimentally, and the experimental results show that higher measurement accuracy can be attained by selecting large amplitude points.Presented at 1996 International Workshop on Interferometry (IWI ‘96), August 27–29, Saitama, Japan.     

Velocity measurement of clay intrusion through a sudden contraction step using a tagging pulse sequence

Magnetic resonance imaging (MRI) with a spatial tagging sequence was used to measure the velocity distribution of clay that was forced past a sudden contraction. A spatial tagging sequence provided magnetic resonance images of clay that allowed measurement of the velocity distribution in the clay, which can provide profound insights on the deformation process of clay during the intrusion process. The experiments were conducted using a specially-designed vessel that could operate at up to 30 MPa. The vessel offers a rectangle test section with a sudden contraction step that had a ratio of contraction of 2:1. The vessel was installed into a commercial magnetic resonance imaging equipment and then the fluid motion of clay flowing into the narrow contracted channel was quantitatively investigated to examine behaviors of flowing clay as non-Newtonian fluid. MRI results are compared with those obtained by computational fluid dynamics (CFD) calculation. Velocity distributions obtained from each tag displacement did not well agree with those predicted by CFD results near the contraction step where the fluid accelerated rapidly. However, a post-processing on calculation results, in which virtual tag displacement is calculated, gave better agreement with experiment and enabled us to compare MRI results with CFD results.     

Rectification in substituted atomic wires: a theoretical insight

Recently, there have been discussions that the giant diode property found experimentally in diblock molecular junctions could be enhanced by the many-body electron correlation effect beyond the mean field theory. In addition, the effect of electron-phonon scattering on an electric current through the diode molecule, measured by inelastic tunneling spectroscopy (IETS), was found to be symmetric with respect to the voltage sign change even though the current is asymmetric. The reason for this behavior is a matter of speculation. In order to clarify whether or not this feature is limited to organic molecules in the off-resonant tunneling region, we discuss the current asymmetry effect on IETS in the resonant region. We introduced heterogeneous atoms into an atomic wire and found that IETS becomes asymmetric in this substituted atomic wire case. Our conclusion gives the other example of intrinsic differences between organic molecules and metallic wires. While the contribution of electron-phonon scattering to IETS is not affected by the current asymmetry in the former case, it is affected in the latter case. The importance of the contribution of the electron-hole excitation to phonon damping in bringing about the current asymmetry effect in IETS in the latter case is discussed.     

Surface relaxation of polarized Xe atoms dissolved in deuterated ethanol

We measured the spin relaxation of polarized xenon atoms dissolved in deuterated ethanol. Surface relaxation was suppressed by coating the cell walls with deuterated eicosane. From the dependence of the decay rate on temperature and static magnetic field, we obtained the correlation time of random fluctuations of the local field at the liquid-solid interface. By varying the cell volume, the wall coating, and the surface area of the eicosane, we measured the contribution of the spin-rotation interaction to the relaxation. The use of both deuterated molecules enables us to distinguish surface relaxation from the magnetic dipole-dipole and spin-rotation interactions in solution.