Polymerization of 1-azabicyclo[4.2.0]octane with chiral tricyclic amino acid esters for obtaining biomedical materials

<正>The cationic ring-opening polymerization of 1-azabicyclo[4.2.0]octane,commonly called conidine(1) was studied with some chiral tricyclic amino acid esters(6a-6f) as acylation agents to obtain optically active biopolymers(8a-8f) of the different masses.The structures of the new compounds were confirmed by FT-IR,UV and NMR,and the distribution index of the molecular weights of the polymers was determined from SEC measurements.     

Spectroscopic investigations and quantum chemical computational study of (E)-4-methoxy-2-[(p-tolylimino)methyl]phenol

In this work the electronic structure of (E)-4-methoxy-2-[(p-tolylimino)methyl]phenol has been characterized by the B3LYP/6-31G(d) level by using density functional theory. The experimental infrared and electronic absorption spectra have been obtained and compared with the theoretically obtained ones. Molecular electrostatic potential map has been evaluated; natural bond orbital and frontier molecular orbitals analysis have been performed from the optimized geometry. The energetic behavior of the title compound has been examined in solvent media using polarizable continuum model. The non-linear optical properties have been computed with the same level of theory. In addition, the changes of thermodynamic properties have been obtained in the range of 100-500 K.     

反气相色谱法测定聚环己基丙烯酸甲酯的热力学性质（英文）

In this work,some thermodynamic properties of poly(cyclohexyl methacrylate)were studied by inverse gas chromatography(IGC).For this purpose,the polymeric substance was coated on Chromosorb W and which was filled into a glass column.The retention times(tr)of the probes were determined from the interactions of poly(cyclohexyl methacrylate)with n-pentane,n-hexane,n-heptane,n-octane,n-decane,methanol,ethanol,2-propanol,butanol,acetone,ethyl methyl ketone,benzene,toluene and o-xylene by IGC technique.Then,the specific volume(V0g)was determined for each probe molecule.By using(1/T;ln V0g)graphics,the glass transition temperature of poly(cyclohexyl methacrylate)was found to be 373 K.The adsorption heat under the glass transition temperature(ΔHa),and partial molar heat of sorption above the glass transition(ΔHS1),partial molar free energy of sorption(ΔGS1)and partial molar entropy of sorption(ΔSS1)belonging to sorption for every probe were calculated.The partial molar heat of mixing at infinite dilution(ΔH∞1),partial molar free energy of mixing at infinite dilution(ΔG∞1),Flory-Huggins interaction parameter(χ∞12)and weight fraction activity coefficient(a1/w1)∞values of polymer-solute systems were calculated at different column temperatures.The solubility parameters(δ2)of the polymer were obtained by IGC technique.     

An Electrochemical Assay for Monitoring Differentiation of the Osteoblastic Cell Line (MBA‐15) on the Sensor Chip

An electrochemical assay for the indication of the activity of the cell bound differentiation marker alkaline phosphatase (ALP) is proposed using voltammetry on an in‐vitro cell culture. The basis of the assay is cultivation of cells on gold microelectrodes in wells of a microplate, catalytic hydrolysis of p‐aminophenyl phosphate by ALP and indication of p‐aminophenol oxidation by square wave voltammetry (SWV) with the sensors onto which the cells attached. The morphology of the bone marrow stromal cell line (MBA‐15) on the electrode surface was investigated and it exhibited in vitro osteogenic characteristics. Since ALP is expressed on the cell surface in early differentiation stage of osteoblastic cells, its activity was followed after different culture times over a period of 144 h by recording repetitive voltammograms at different time points upon addition of the substrate p‐aminophenyl phosphate. The ALP activity was estimated from the signal increase related to formation rate of p‐aminophenol and the number of cells. The highest value was measured at 120 h, when the cells reached confluence. The results of the electrochemical activity assay are consistent with the colorimetric acquired value from p‐nitrophenol formation rate.