Four spectroradiometers located at latitudes from 55 degrees to 90 degrees S conducted near-continuous measurements of ground-level solar ultraviolet irradiance from 1990 through 2001. The behavior during months from October through December is of special interest because this period includes the springtime loss in column ozone and the naturally large irradiances of early summer. Monthly integrated irradiances using biological weightings for erythema and damage to DNA show a distortion of the normal annual cycle in irradiance, with enhanced values occurring in October and November. In some cases, these irradiances exceed those near summer solstice in December. Changes in local cloudiness and column ozone both contribute significantly to interannual variability in erythemal irradiance. This is particularly the case at Palmer Station, near 65 degrees S, where the monthly integrated erythemal irradiance in November 1997 was more than double that observed 5 years earlier. In general, at sites on the Antarctic continent, interannual variability in monthly integrated erythemal irradiance is greatest in November, when the observation for any given year can fall 40% above or below the multiyear mean. Near the tip of South America, interannual variability is approximately half that seen in Antarctica. 相似文献
Sulfotyrosine and phosphotyrosine are two post-translational modifications present in higher eukaryotes. A simple and direct mass spectrometry method to distinguish between these modifications is crucial to advance our understanding of the sulfoproteome. While sulfation and phosphorylation are nominally isobaric, the accurate mass of the sulfuryl moiety is 9.6 mDa less than the phosphoryl moiety. Based on this difference, we have used an Orbitrap Fusion Lumos mass spectrometer to characterize, resolve, and distinguish between sulfotyrosine and phosphotyrosine modifications using a set of model peptides. Multiple fragmentation techniques, namely HCD, CID, ETD, ETciD, and EThcD, have been used to compare the different fragmentation behaviors between peptides modified with these species. Sulfotyrosine undergoes neutral loss using HCD and CID, but the sulfuryl moiety is largely stable under ETD. In contrast, phosphotyrosine is stable during fragmentation using all these methods. This differential stability provides a mechanism to distinguish sulfopeptides from phosphopeptides. Based on the rigorous characterization presented herein, this work serves as a model for accurate identification of phosphotyrosine and, more challenging, sulfotyrosine, in complex proteomic samples.
A novel approach for reversibly switching the conformation of short constraint λ-helical peptides through chemselective alkylation of the in-tether thioether and dealkylation of the chiral sulfonium was developed and it provides a valuable modifiable site to functionalize the peptides. 相似文献
Dissolution of cellulose is the key challenge in its applications. It has been discovered that spruce cellulose with high molecular weight (4.10 × 105 g mol?1) can be dissolved in 64 wt% H2SO4 aqueous solution at low temperature within 2 min, and the cellulose concentration in solution can reach as high as 5 % (w/v). FT-IR spectra and XRD spectra proved that it is a direct solvent for cellulose rather than a derivative aqueous solution system. The cold H2SO4 aqueous solution broke the hydrogen bonds among cellulose molecules and the low temperature dramatically slowed down the hydrolysis, which led to the dissolution of cellulose. The resultant cellulose solution was relatively stable, and the molecular weight of cellulose only slightly decreased after storage at ?20 °C for 1 h. Due to the high molecular weight of cellulose, cellulose solution could form regenerated films with good mechanical properties and transparency at low concentration (2 % w/v). This work has not only provided the new evidence of cellulose dissolution which facilitated the development of cellulose solvent, but also suggested a convenient way to directly transfer cellulose with high molecular weight into materials without structure modifications. 相似文献
Science China Chemistry - Trace Ir(III) complex enhanced aggregation-induced electrochemiluminescence (AIECL) of poly-tetraphenylethene (pTPE) in aqueous media was investigated for the first time.... 相似文献