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41.
We present a novel sensing scheme for detecting the effects of unburned fossil fuels by integrating microarray technology and dielectrophoresis to develop single-neuron arrays. These arrays have the capability to sense and identify the two fuels, at parts per billion (ppb) concentrations, as well to determine the associated physiological changes at the single-cell level. Identification is achieved through frequency domain analysis of the measured changes to the extracellular electrical activity due to the effect of the fossil fuels. This yields unique electrical identifiers known as "signature patterns". Simultaneous optical visualization to the physiological changes is obtained by specific fluorescent staining. The correlation between the signature patterns and the cellular biological behavior establishes the veracity of this identification technique. 相似文献
42.
J. Edward Schneider Jr. Tahereh Tabatabaie Lindsay Maidt Renee Horning Smith Xuan Nguyen Quentin Pye Robert A. Floyd 《Photochemistry and photobiology》1998,67(3):350-357
A spectrum of oxidative lesions was observed in a bacteriophage-based model system that is very sensitive to the photodynamic activity of selected dyes. When suspensions of the intact bacteriophage Qβ were exposed to methylene blue plus light (MB+L), inactivating events, or "hits" occurred that were oxygen-dependent and that were associated with the formation of several specific lesions: (1) carbonyl moieties on proteins, (2) 8-oxo-7,8-dihydroguanine (8-oxoGua), and (3) single-strand breaks (ssb) in the RNA genome and (4) RNA-protein crosslinks. Formation of carbonyl groups associated with protein in the Qβ phage preparation correlated positively with photoinactivation of the phage with increasing doses of either of the sensitizers MB or rose bengal. Strand breaks in the Qβ genomic RNA were observable at high MB concentrations but appeared not to be significant at the lower concentrations of MB, as full-length Qβ RNA was observable well beyond the 99% inactivation point in MB dosage. It was shown that the number of 8-oxoGua lesions were unlikely to be sufficient to account for the number of lethal events. Following exposure to MB+L, crosslink formation between Qβ RNA and protein was observed by virtue of the location of RNA at the interface of phenol-aqueous extractions of phage suspensions. A significant increase over background of RNA-protein complexes (including full-length Qβ RNA) was observed at the lowest concentration of MB tested (0.5 μ M ), which corresponded roughly to an average of 2 lethal hits per phage or approximately 13% survival compared to the zero MB control (100% survival). Due to its close correlation with Qβ inactivation and its expected lethality, RNA-protein crosslink formation may be important as an inactivating lesion in bacteriophage Qβ following MB+L exposure. 相似文献
43.
Four new derivatives of podophyllotoxin, N'-podophyllic acid-N-[3-(2, 2, 5, 5-te-tramethyl-pyrrolinenyloxy)] semicarbazide(GP-11, 6), podophyllic acid [3-(2,2,5,5-te-tramethyl-pyrrolinenyloxy)]hydrazone (GP-12, 7), podophyllic acid-[4-(2, 2, 6, 6-tetramethyl-1-hydroxy piperidine)]hydrazone(GP-1-OH, 8) and podophyllic acid[4-(2,2, 6, 6-tetramethyl piperidine)]hydrazone(GP-1-H, 9) were synthesized. The inhibition effect of the four new compounds on L-1210 cells were determined. The antitumor activity and toxicity of GP-1(2), GP-1-OH(8), GP-1-H(9) and VP-16-213(1) were discussed. 相似文献
44.
45.
采用水热法合成了2个新的配位聚合物[Co(L)0.5(1,4-bib)(H2O)3]n(1)和[Ni(L)0.5(1,4-bib)(H2O)3]n(2)(H4L=1,4-二(2,6-二甲基-3,5-二羧基吡啶基)苯,1,4-bib=1,4-双(1-咪唑基)苯),通过X射线单晶及粉末衍射、元素分析、红外光谱分析和热重分析对其结构进行表征。结构分析表明配合物1和2为异质同构,均为一维结构。通过变温磁化率测量发现,配合物1和2中的Co(Ⅱ)/Ni(Ⅱ)离子之间存在反铁磁相互作用。此外,配合物1可通过荧光猝灭法检测头孢克肟(CEF),配合物2可用来检测四环素(TET),该方法灵敏度高、选择性好,其检出限分别为0.86 μmol·L-1(CEF)和0.49 μmol·L-1(TET)。 相似文献
46.
Tingting Zhou Ziwen Deng Qing Wang Hui Li Shun Li Xuanming Xu Yusun Zhou Shukai Sun Chao Xuan Qingwu Tian Limin Lun 《Molecules (Basel, Switzerland)》2022,27(18)
In this work, novel selective recognition materials, namely magnetic molecularly imprinted polymers (MMIPs), were prepared. The recognition materials were used as pretreatment materials for magnetic molecularly imprinted solid-phase extraction (MSPE) to achieve the efficient adsorption, selective recognition, and rapid magnetic separation of methotrexate (MTX) in the patients’ plasma. This method was combined with high-performance liquid chromatography–ultraviolet detection (HPLC–UV) to achieve accurate and rapid detection of the plasma MTX concentration, providing a new method for the clinical detection and monitoring of the MTX concentration. The MMIPs for the selective adsorption of MTX were prepared by the sol–gel method. The materials were characterized by transmission electron microscopy, Fourier transform-infrared spectrometry, X-ray diffractometry, and X-ray photoelectron spectrometry. The MTX adsorption properties of the MMIPs were evaluated using static, dynamic, and selective adsorption experiments. On this basis, the extraction conditions were optimized systematically. The adsorption capacity of MMIPs for MTX was 39.56 mgg−1, the imprinting factor was 9.40, and the adsorption equilibrium time was 60 min. The optimal extraction conditions were as follows: the amount of MMIP was 100 mg, the loading time was 120 min, the leachate was 8:2 (v/v) water–methanol, the eluent was 4:1 (v/v) methanol–acetic acid, and the elution time was 60 min. MTX was linear in the range of 0.00005–0.25 mg mL−1, and the detection limit was 12.51 ng mL−1. The accuracy of the MSPE–HPLC–UV method for MTX detection was excellent, and the result was consistent with that of a drug concentration analyzer. 相似文献
47.
In this study, the protein bioaccessibility of soymilk gels produced by the addition of glu-cono-δ-lactone (GDL) and fermentation with lactic acid bacteria (LAB) was examined using an in vitro gastrointestinal simulated digestion model. The in vitro protein digestibility, soluble protein content, free amino acids contents, degree of hydrolysis, electrophoretic patterns, and peptide content were measured. The results suggested that acid-induced soymilk gel generated by GDL (SG) showed considerably reduced in vitro protein digestibility of 75.33 ± 1.00% compared to the soymilk gel induced by LAB (SL) of 80.57 ± 1.53% (p < 0.05). During the gastric digestion stage, dramatically higher (p < 0.05) soluble protein contents were observed in the SG (4.79–5.05 mg/mL) than that of SL (4.31–4.35 mg/mL). However, during the later intestinal digestion phase, the results were the opposite. At the end of the gastrointestinal digestion phase, the content of small peptides was not significantly different (p > 0.05) between the SL (2.15 ± 0.03 mg/mL) and SG (2.17 ± 0.01 mg/mL), but SL showed higher content of free amino acids (20.637 g/L) than that of SG (19.851 g/L). In general, soymilk gel induced by LAB had a higher protein bioaccessibility than the soymilk gel coagulated by GDL. 相似文献
48.
通过纳米二氧化硅的硅烷化改性,使其在高矿化度盐水中可以稳定存在的前提下,研究了改性纳米颗粒与阳离子表面活性剂十二烷基三甲基氯化铵混合体系的溶液稳定性及协同稳定CO2泡沫的效果.研究结果表明,无机盐离子对改性纳米颗粒与阳离子表面活性剂间的静电吸引力具有屏蔽作用,且矿化度越高,屏蔽效果越明显,从而混合溶液更易于在高盐水中稳定;纳米颗粒表面的活性剂吸附层受二者浓度的影响,进而影响了颗粒的亲/疏水性;当混合体系中的表面活性剂浓度低于临界胶束浓度(CMC)时,混合溶液与CO2的界面张力高于单独活性剂溶液,而当活性剂浓度高于CMC时,对CO2-溶液界面张力几乎无影响,最低界面张力可降至6 mN/m左右;改性纳米颗粒的加入可以进一步提高CO2体相泡沫半衰期一倍以上,但受二者浓度比例的影响;纳米颗粒的加入有效提高了多孔介质中泡沫的表观黏度,最大增幅由20 mPa·s增至55 mPa·s左右,泡沫黏度增加接近3倍,增强了CO2泡沫驱的封堵作用. 相似文献
49.
磁流变弹性体(magnetorheological elastomer,MRE)因其独特的场相关性能在工程应用领域引起了广泛关注.论文介绍了MRE的最新发展,重点介绍导电性MRE在材料设计、力学行为和实际应用等方面的研究现状.论文从高性能导电性MRE的研制开始,讨论了材料与结构设计中涉及的关键问题;介绍了导电性MRE材料在磁场作用下的磁、力、电学行为的耦合作用机理和相关物理模型;之后对导电性MRE器件的研究现状进行了总结;最后展望了导电性MRE的发展潜力和面临的挑战. 相似文献
50.
Xuan Shi Yu Sun Junhui Liu Wencai Liu Yan Xing Zhilong Xiu Yuesheng Dong 《Molecules (Basel, Switzerland)》2023,28(1)
Chemical epigenetic regulation (CER) is an effective method to activate the silent pathway of fungal secondary metabolite synthesis. However, conventional methods for CER study are laborious and time-consuming. In the meantime, the overall profile of the secondary metabolites in the fungi treated by the CER reagent is not well characterized. In this study, suberohydroxamic acid (SBHA), a histone deacetylase inhibitor, was added to a culture of Aspergillus aculeatus DL1011 and a new strategy based on LC-MS/MS analysis integrated with various metabolomic tools (MetaboAnalyst, MS-DIAL, SIRIUS and GNPS) was developed to characterize the profile of induced metabolites. As a result, 13.6%, 29.5% and 27.2% of metabolites were identified as newly biosynthesized, increasing and decreasing in abundance by CER, respectively. The structures of the 18 newly induced secondary metabolites were further identified by the new strategy to demonstrate that 72.2% of them (1 novel compound and 12 known compounds) were first discovered in A. aculeatus upon SBHA treatment. The accuracy of the new approach was confirmed by purification and NMR data analysis of major newly biosynthesized secondary metabolites. The bioassay showed that the newly biosynthesized compounds, roseopurpurin analogues, showed selective activities against DPPH scavenging, cytotoxicity and SHP1 inhibition. Our research demonstrated that CER was beneficial for changing the secondary metabolic profile of fungi and was an effective means of increasing the diversity of active metabolites. Our work also supplied a metabolomic strategy to characterize the profile changes and determine the newly induced compounds in the secondary metabolites of fungi treated with the chemical epigenetic regulator. 相似文献