PNA for one-base differentiating protection of DNA from nuclease and its use for SNPs detection

By the combination of peptide nucleic acid (PNA) with single-stranded DNA specific nucleases, alteration of a single base to another in DNA has been detected with high accuracy. Only the DNAs in DNA/PNA duplexes involving a mismatch are efficiently hydrolyzed by these enzymes, whereas fully matching sequences are kept intact. This difference is visually scored by adding 3,3'-diethylthiadicarbocyanine, which changes its color from blue to purple upon binding to DNA/PNA duplexes. These findings are applied to the convenient and straightforward detection of single nucleotide polymorphisms (SNPs). When the target site in the sample DNA is completely complementary with the PNA, a notable amount of DNA/PNA duplex remains and thus the solution exhibits purple color. In the presence of even one mismatch between PNA and DNA, however, the DNA is completely digested by the enzyme and therefore the dye shows its intrinsic blue color. The SNPs in the apolipoprotein E gene of human DNA have been successfully genotyped by this method.     

The solid-phase synthesis and use of N-monosubstituted piperazines in chemical library synthesis

An efficient solid-phase synthesis of mono-N-substituted piperazines is presented. The key transformation involves a selective borane amide bond reduction in the presence of a carbamate resin linkage. This synthetic route takes advantage of the large diverse pool of commercially available carboxylic acids, acid chlorides, and sulfonyl chlorides. The solid-phase approach facilitates parallel processing by eliminating the need for column chromatography after each synthetic step. The N-monosubstituted piperazines were shown to react with polymeric activated tetrafluorophenol (TFP) reagents to generate arrays of amides and sulfonamides in good purity for biological testing.     

Simultaneous determination and pharmacokinetic studies on (3,4-Dihydroxyphenyl)-lactic acid and protocatechuic aldehyde in rat serum after oral administration of Radix Salviae miltiorrhizae extract

A simple and sensitive high-performance liquid chromatography method is developed for the simultaneous determination of (3,4-dihydroxyphenyl)-lactic acid (Dhpl) and protocatechuic aldehyde (Pal) in rat serum after oral administration of Radix Salviae miltiorrhizae extract. Serum samples are acidified with hydrochloric acid and extracted with ethyl acetate. Analysis of the extract is performed on a reversed-phase column and a mobile phase of 0.02% phosphoric acid-acetonitrile (91:9, v/v) with UV detection at 280 nm. Standard curves are linear in the range of 1.47-456.96 microg/mL for Dhpl and 0.124-7.936 microg/mL for Pal. For both regression coefficients, r(2) is greater than 0.993. Mean recovery is determined to be 75.23% and 84.06%, respectively, by analyzing serum standard containing 7.14, 57.12, and 228.48 microg/mL of Dhpl and 0.124, 0.992, and 3.968 microg/mL of Pal. The intraday precision (relative standard deviation) ranges from 3.91% to 12.03% at concentrations of 1.43, 57.12, and 228.48 microg/mL for Dhpl and 3.79% to 8.12% at concentrations of 0.124, 0.992, and 3.968 microg/mL for Pal. The interday precision (relative standard deviation) ranges from 5.06% to 9.93% for Dhpl and 3.05% to 10.00% for Pal, respectively, at the same three concentrations. This validated assay is applied to the determination of Dhpl and Pal concentrations and used to take a limited view of the pharmacokinetic profile in rat serum after oral administration of Radix Salviae miltiorrhizae extract.     

Precise rate in the law of iterated logarithm for <Emphasis Type="Italic">ρ</Emphasis>-mixing sequence

Let {X, X _n;n≥1} be a strictly stationary sequence of ρ-mixing random variables with mean zero and finite variance. Set . Suppose lim _n→∞ and , where d=2, if −1<b<0 and d>2(b+1), if b≥0. It is proved that, for any b>−1,

Gomory integer programs

 The set of all group relaxations of an integer program contains certain special members called Gomory relaxations. A family of integer programs with a fixed coefficient matrix and cost vector but varying right hand sides is a Gomory family if every program in the family can be solved by one of its Gomory relaxations. In this paper, we characterize Gomory families. Every TDI system gives a Gomory family, and we construct Gomory families from matrices whose columns form a Hilbert basis for the cone they generate. The existence of Gomory families is related to the Hilbert covering problems that arose from the conjectures of Seb?. Connections to commutative algebra are outlined at the end. Received: May 17, 2001 / Accepted: February 7, 2002 Published online: April 24, 2003 RID="⋆" ID="⋆" Research partially supported by NSF grant DMS-0100141.     

QUALITATIVE THEORY OF THE KUKLES SYSTEMS:(Ⅰ) NUMBER OF CRITICAL POINTS

The special cubic system (a natural and simPlest generaIization of thequadratic system of C1ass (l) to cubic system)f.'x = yl y = --x 6y a,x' a2xy a,y' a#x' a,x'y a.xy' a,y' (1)was first studied by the Russian Mathematician I.S.KukIes [ll on the Ilecessaryand sufficient conditions fot O(0,0) to be a ccnter of (1). After [ll, the sameproblem and also the order of O as a fine fOcus were also studied iIl [2--l0] and[13-19], so (l) is now called as a "Kukles system". In l988-…     

Geometric Dynamics

A kinematic differential system on a Riemann (or semi-Riemann) manifold induces a Lorentz-Udrite world-force law, i.e., any local group with one parameter (any local flow) on a Riemann (or semi-Riemann) manifold induces the dynamics of the given vector field or of an associated particle, which will be called geometric dynamics.The cases of Riemann-Jacobi or Riemann-Jacobi-Lagrange structures are imposed by the behavior of an external tensor field of type (1,1). The case of the Finsler-Jacobi structure appears if the initial metric is chosen such that the energy of the given vector field is constant (Sec. 1). At the end of Sec. 1 are formulated open problems regarding some extensions of geometric dynamics.Adequate structures on the tangent bundle describe the geometric dynamics in the Hamilton language (Sec. 2).Section 3 proves the existence of a Finsler-Jacobi structure induced by an almost contact metric structure.The theory is applied to electromagnetic dynamical systems (the starting point of our theory), offering new principles of unification of the gravitation and the electromagnetism. Also, here, one enounces open problems regarding the geometric dynamics induced by the electric intensity and magnetizing force (Sec. 4).From the geometrical point of view, we create a wider class of Riemann-Jacobi, Riemann-Jacobi-Lagrange, or Finsler-Jacobi manifolds ensuring that all trajectories of a given vector field are geodesics. Having T₁M²ⁿ⁺¹ in mind, the problem of creating a wider class of Riemannian manifolds, in which there exists a vector field such that (1) all trajectories of the vector field are geodesics; (2) the flow defined by is incompressible; (3) the condition which corresponds to the property that is the associate vector field of the contact structure is satisfied;was studied intensively by S. Sasaki. The results were not satisfactory, but Sasaki discovered (, , )-structures [10].AMS Subject Classification (1991): 70H35, 53C22, 58F25, 83C22     

Correlation of substituent effects and energy levels of the two lowest excited states of the azulenic chromophore

[structure: see text] Experimental and calculated data show that the relative energy levels of the two lowest excited states of azulene are sensitive to the nature and position of substituents on the nonalternant hydrocarbon. Extending such investigations led to a rational explanation for some of the baffling data on azulenic bacteriorhodopsin analogues in the literature.     

Target strength of an oily deep-water fish, orange roughy (Hoplostethus atlanticus). II. Modeling

Orange roughy consist of approximately 18% lipids by weight, mostly as wax esters, and the lipids must be taken into account when modeling target strength. A deformed cylinder model incorporating the effect of temperature and pressure on sound speed through wax ester was used to scale experimental measurements of target strength to the temperatures and pressures where orange roughy live (approximately 6 degrees C, depths approximately 800-1300 m). The effect of decreasing temperature and increasing pressure is to increase the sound speed in orange roughy lipids. Modeling shows that the net effect of this is to reduce tilt-averaged target strength, 'TS', by approximately 2 dB. Adjusting experimental results to compensate for temperature and pressure effects gives a predicted 'TS' for a 35-cm orange roughy of -48.3 dB. Adjusting in situ estimates of orange roughy 'TS' for avoidance behavior [McClatchie et al., J. Acoust. Soc. Am. 106, 131-142 (1999)] suggests the correct 'TS' is approximately -47.5 dB, rather than -50 dB as previously reported [Kloser et al., ICES J. Mar. Sci. 54, 60-71 (1997)]. We conclude that experimental and in situ estimates now converge at a 'TS' of approximately -48 dB for a 35-cm fish.     

Enhancement and inhibition of acoustic radiation in spherical cavities

Acoustic radiation from a source located inside layered cavities is studied using the transfer matrix method. It is shown that the radiation can be either enhanced or inhibited, depending on the characteristics and the material composition of the cavities. The analysis predicts well-defined spectral peaks and nodes in some cavities. It is found that significant enhancement and inhibition are possible by varying acoustic parameters. The results also show that the strength of the radiating source can be significantly modified due to the presence of layered structures. The link to the similar phenomena in the optical cavities is discussed.