Continuous-wave, single-frequency intracavity singly resonant optical parametric oscillator at 1.5-μm wavelength

We present a 1.5μm continuous-wave （CW） single-frequency intracavity singly resonant optical parametric oscillator （SRO） based on periodically poled lithium niobate （PPLN）. The SRO is placed inside the ring cavity of a single-frequency 1.06μm Nd：YVO4 laser pumped by a laser diode. The device delivers a maximum single-frequency output power of 310 mW at a resonant signal wavelength of 1.57 μm. The signal wave could be tuned from 1.57 to 1.59 μm by temperature tuning of PPLN crystal over the range of 130 - 170℃.     

The synthesis of complexes using precursor complexes with ferrocenyl carboxylate units as building blocks

Using ferrocenyl carboxylates as functional ligands, we synthesized a mononuclear complex [Zn(η²-OOCCHCHFc)₂(CH₃OH)₂] (1) and a binuclear complex [Cd(bafca)₂(H₂O)₂]₂ (2) (bafca⁻ = α-benzamido-β-ferrocenylacrylic carboxylate) as precursor complexes. Investigation on the substitution reaction of precursor complexes as building blocks in solution-state, four complexes [Zn(OOCCHCHFc)₂(bbbm)]_n (1a), {[Zn(OOCCHCHFc)(ntb)](CH₃OH)} (1b), [Cd(bafca)₂(2,2′-bpy)]₂ · 9H₂O (2a) and {[Cd(bafca)₂(bbbm)(CH₃OH)₂] · 6CH₃OH}_n (2b) were obtained (bbbm = 1,1-(1,4-Butanediyl)bis-1H-benzimidazole, ntb = N,N-bis(1H-benzimidazol-2-ylmethyl)-1H-Benzimidazole-2-methanamine and 2,2′-bpy = 2,2′-bipyridine). As anticipated, the structural integrity of precursor complexes can be maintained in these four complexes. It indicates that we can synthesize the desired complexes with the destination structures by using precursor complexes as building blocks and choosing appropriate auxiliary ligands. In addition, the electrochemical properties of all complexes were investigated, and it can be seen from the results that half-wave potentials of these complexes are slightly higher than that of the corresponding ligand.     

基于核磁共振氢谱代谢组学研究黄连解毒汤对胰岛素抵抗大鼠棕色脂肪组织代谢组的影响

采用基于核磁共振氢谱(¹H NMR)的代谢组学方法, 研究了黄连解毒汤(HJD)对高果糖诱导胰岛素抵抗大鼠模型棕色脂肪代谢组的影响. 选取Wistar大鼠32只, 适应7 d后随机分为正常对照组、 模型组、 阳性药物对照组和黄连解毒汤组, 每组8只. 正常对照组给予纯净水喂养, 其它3组给予100 g/L的果糖水喂饲. 28 d后, 4组大鼠除了继续给予100 g/L的果糖水喂养外, 阳性对照组和黄连解毒汤组同时分别给予阿托伐他汀10 mg/(kg·d)和HJD水煎剂3.175 g/(kg·d)灌胃, 正常对照组和模型组给予一定体积的生理盐水灌胃, 整个实验持续56 d. 取各组大鼠棕色脂肪组织(BAT), 采集各组组织提取液的¹H NMR谱, 运用主成分分析法(PCA)分析. 与正常对照组相比, 在模型组中乳酸、 胆碱、 磷脂胆碱/甘油磷脂胆碱、 肌酸/肌酸酐、 牛磺酸和肌苷的含量升高, 脂质含量降低; 黄连解毒汤组逆转了模型组中上述各代谢物的变化, 且引起肌醇升高, 均具有统计学意义. 实验结果表明, 黄连解毒汤能够逆转机体能量代谢、 减轻细胞膜受损以及降低肝肾损伤, 初步阐明了黄连解毒汤对胰岛素抵抗状态下棕色脂肪组织代谢的调控作用.     

INVARIANT TESTS OF EXISTENCE OF THE LINEAR RELATIONSHIP WITH LEFT O(n)-INVARIANT ERRORS

This paper gives the invariant tests of the existence of a linear relationship among row vectors of the mean matrix of the multivariate linear models with the left O(n)-invariant errors. Some asymptotic properties of these testing methods are also discussed.     

Sulforaphane Inhibits Osteoclastogenesis via Suppression of the Autophagic Pathway

Previous studies have demonstrated that sulforaphane (SFN) is a promising agent against osteoclastic bone destruction. However, the mechanism underlying its anti-osteoclastogenic activity is still unclear. Herein, for the first time, we explored the potential role of autophagy in SFN-mediated anti-osteoclastogenesis in vitro and in vivo. We established an osteoclastogenesis model using receptor activator of nuclear factor kappa-β ligand (RANKL)-induced RAW264.7 cells and bone marrow macrophages (BMMs). Tartrate-resistant acid phosphatase (TRAP) staining showed the formation of osteoclasts. We observed autophagosomes by transmission electron microscopy (TEM). In vitro, we found that SFN inhibited osteoclastogenesis (number of osteoclasts: 22.67 ± 0.88 in the SFN (0) group vs. 20.33 ± 1.45 in the SFN (1 μM) group vs. 13.00 ± 1.00 in the SFN (2.5 μM) group vs. 6.66 ± 1.20 in the SFN (2.5 μM) group), decreased the number of autophagosomes, and suppressed the accumulation of several autophagic proteins in osteoclast precursors. The activation of autophagy by rapamycin (RAP) almost reversed the SFN-elicited anti-osteoclastogenesis (number of osteoclasts: 22.67 ± 0.88 in the control group vs. 13.00 ± 1.00 in the SFN group vs. 17.33 ± 0.33 in the SFN+RAP group). Furthermore, Western blot (WB) analysis revealed that SFN inhibited the phosphorylation of c-Jun N-terminal kinase (JNK). The JNK activator anisomycin significantly promoted autophagy, whereas the inhibitor SP600125 markedly suppressed autophagic activation in pre-osteoclasts. Microcomputed tomography (CT), immunohistochemistry (IHC), and immunofluorescence (IF) were used to analyze the results in vivo. Consistent with the in vitro results, we found that the administration of SFN could decrease the number of osteoclasts and the expression of autophagic light chain 3 (LC3) and protect against lipopolysaccharide (LPS)-induced calvarial erosion. Our findings highlight autophagy as a crucial mechanism of SFN-mediated anti-osteoclastogenesis and show that the JNK signaling pathway participates in this process.     

Catalytic applications of Cu-containing MOFs based on N-heterocyclic ligand in the oxidative coupling of 2,6-dimethylphenol

Two Cu^II complexes bearing a N-heterocyclic ligand, namely [Cu(SO₄)(pbbm)]_n (1) and {[Cu(Ac)₂(pbbm)] · CH₃OH}_n (2) (pbbm = 1,1′-(1,5-pentanediyl)bis-1H-benzimidazole) have been synthesized with the aim of exploiting new and potent catalysts. Single crystal X-ray diffraction shows that new polymeric complex 1 features 1-D double-chain framework. The catalytic studies on 1 and 2 indicate that they are efficient homogeneous catalysts for the oxidative coupling of 2,6-dimethylphenol (DMP) to poly(1,4-phenylene ether) (PPE) and diphenoquinone (DPQ) with H₂O₂ as oxidant and NaOMe as co-catalyst at room temperature. Optimal reaction conditions are obtained by examining the effects of solvent, the reaction time, temperature as well as the amounts of co-catalyst, catalyst and oxidant. Under the optimal conditions, the selectivity to PPE is almost up to 90% for both complexes, and the conversion of DMP is 85% for 1 and 90% for 2, comparable to those observed for highly active catalyst systems in the literature. Further comparison of their catalytic performances with those of the corresponding copper salt together with organic ligand, copper salt alone and free ligand reveals that the coordination of ligand to Cu^II ion plays a key role in generating the superior reactivities of complexes.     

纤维素固定化血红蛋白研究

纤维素是一种重要的化工原料,由于来源广泛、价廉以及良好的再生性、反应性和通用性,被广泛用做载体材料．在酶的分离纯化,蛋白质分离纯化,亲合色谱固定相,拆分手性化合物等应用领域,往往采用球形纤维素或膜状纤维素形式．而在水吸附剂,分离回收金属离子,从海水中...     

以琼脂凝胶固定热聚IgG制备类风湿关节炎免疫吸附剂的研究

以环氧氯丙烷对琼脂凝胶珠进行活化反应后键联热聚ＩｇＧ,制成一种新型类风湿关节炎免疫吸附剂。确定了最佳制备条件,使凝胶上环氧基的含量达１１０μｍｏｌ／ｇ,对热苯ＩｇＧ的固定量达６ｍｇ／ｇ。在体外条件下吸附剂对三种类风湿因子ＩｇＭＲＦ,ＩｇＧＲＦ及ＩｇＡＲＦ的吸附量分别达３４００,２２５０和２４００ＩＵ／ｇ,具有良好的应用前景。     

鸟嘌呤、鸟苷及其甲基化衍生物的近红外傅里叶变换表面增强拉曼光谱

本文给出了采用表面增强傅里叶变换拉曼光谱法测定的鸟嘌呤、鸟苷及其甲基化衍生物的拉曼散射。实验结果表明，采用了近红外波长的拉曼散射及傅里叶变换技术成功地获得鸟嘌呤、鸟苷及其甲基化衍生物的水溶液在较低浓度下（0．1～0．01mg／L）不受荧光干扰的拉曼光谱图，其频率与相对强度分布表明，水溶液状态下吸附在Ag膜上的鸟嘌呤（苷）及其衍生物结构中的有关振动谱带r（C＝O）、NH2和杂环上的N获得显著增强。本文对水溶液鸟嘌呤（苷）及其衍生物的FT－Raman谱带的归宿进行了讨论。