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41.
Two new standard reference materials (SRM) for drugs of abuse in human hair have been developed. SRM 2379 consists of hair spiked with cocaine, benzoylecgonine, cocaethylene, phencyclidine, amphetamine, and methamphetamine. SRM 2380 consists of hair spiked with codeine, morphine, monoacetylmorphine, and tetrahydrocannabinol (THC). The SRMs were prepared by soaking the hair in a solution of the target analytes in water-dimethylsulfoxide. The concentration of each analyte was determined using two methods, one based upon gas chromatography/mass spectrometry (GC/MS) and one based upon liquid chromatography/mass spectrometry (LC/MS). Both methods used 0.1 M HCl for extraction of all the analytes from the hair, except for THC, which was extracted with 1 M NaOH. For isolation of the analytes from the extracts, the GC/MS-based methods used different clean-up procedures from those used for the LC/MS-based methods. The results from the two methods were in good agreement with mean differences for the analytes ranging from 4% to 16%. These materials will enable laboratories performing analyses of hair for drugs of abuse to test the accuracy of their methods. 相似文献
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Yibin Pan Lili Yan Qiaoqiao Chen Cheng Wei Yongdong Dai Xiaomei Tong Haiyan Zhu Meifei Lu Yanling Zhang Xiaoying Jin Tai Zhang Xiaona Lin Feng Zhou Songying Zhang 《Experimental & molecular medicine》2021,53(1):52
In early pregnancy, the placenta anchors the conceptus and supports embryonic development and survival. This study aimed to investigate the underlying functions of Shh signaling in recurrent miscarriage (RM), a serious disorder of pregnancy. In the present study, Shh and Gli2 were mainly observed in cytotrophoblasts (CTBs), Ptch was mainly observed in syncytiotrophoblasts (STBs), and Smo and Gli3 were expressed in both CTBs and STBs. Shh signaling was significantly impaired in human placenta tissue from recurrent miscarriage patients compared to that of gestational age-matched normal controls. VEGF-A and CD31 protein levels were also significantly decreased in recurrent miscarriage patients. Furthermore, inhibition of Shh signaling impaired the motility of JAR cells by regulating the expression of Gli2 and Gli3. Intriguingly, inhibition of Shh signaling also triggered autophagy and autolysosome accumulation. Additionally, knockdown of BECN1 reversed Gant61-induced motility inhibition. In conclusion, our results showed that dysfunction of Shh signaling activated autophagy to inhibit trophoblast motility, which suggests the Shh pathway and autophagy as potential targets for RM therapy.Subject terms: Embryology, Endocrine reproductive disorders, Autophagosomes 相似文献
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氢化物发生-原子荧光光谱法测定水中汞的测量不确定度分析 总被引:2,自引:0,他引:2
对氢化物发生-原子荧光光谱法测定水中汞的分析方法的测量不确定度进行了估计。认为在分析的全过程中,标准溶液的配制、校准曲线的绘制以及试样的分析是测量不确定度的三项主要来源。基于这一认识,建立了相应的数学模型,对关系式中的各个变量作了分析并对其不确定度进行量化。最后,按统计学的计算方法给出了合成不确定度和扩展不确定度。 相似文献
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In this paper, new Jacobi elliptic function solutions of
multi-component mKdV equation are obtained directly in a unified
way. When the modulus m→1, those periodic solutions
degenerate as the corresponding hyperbolic function solutions.
Then, to the three-component mKdV equation, five types of
effective solution are presented in detail. 相似文献
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在分析横向型企业集群供应链特点的基础上,侧重于从物流方面阐述了横向型企业集群内制造商与其上下游企业间的供应链关系,以及制造商与制造商之间的供应链关系.同时结合当前横向型企业集群存在的问题,从改善其供应链关系的角度构建了水平集成型虚拟组织供应链模型,并给出了一个应用案例. 相似文献