小白鼠组织和骨中铜锌铁钙镁的测定

本实验利用原子吸收光谱法对小白鼠组织及骨中Ｃｕ，Ｚｎ，Ｆｅ，Ｃａ，Ｍｇ含量进行了测定。样品采用浓ＨＮＯ２和ＨＣｌＯ４湿法消化，并用国家计量局的猪肝（ＧＢＷ０８５５１）作为标准物对测定方法进行了考核，测定值与标准值吻合。方法加收率：Ｃｕ９９－１００％，Ｚｎ１０２－１０３％，Ｆｅ９９－１００％，Ｃａ９９－１０４％，Ｍｇ１０１－１０３％，ＣＶ值为１．７－３．９％。方法简便，快速，准确度高，需样量少，符合     

修饰壳聚糖吸附低密度脂蛋白前后血浆中铜锌钙镁含量的变化

本文采用自制的修饰壳聚糖吸附剂对血浆中的低密度脂蛋白进行吸附后，用火焰原子吸收光谱法检测吸附前后血浆中Cu,Zn,Ca,Mg含量。为了减小血浆的用量，采用微量脉冲法进样，进样体积仅需100－200μl，血浆样品经稀释后直接测定。平均回收率为98．0－101．0％。相对标准偏差为1．89－4．27％。Cu,Zn,Ca,Mg的检出限分别为0.025,0.028,0.023,0.005μg/ml。方法简便，快速，需样量少，可同时多个元素。     

MH/Ni电池用稀土系储氢合金的失效及回收研究

探讨了深度过放电对MH／Ni电池负极储氢合金的影响。发现在过放电后，负极储氢合金的XRD结构图中，除了储氢合金的主相外，还出现了十分明显的Al(OH3),La(OH)3的衍射峰。结合各种情况下储氢合金失效的,原因利用化学处理及再熔炼的方法对失效MH／Ni电池的负极粉进行了回收实验，并对比了回收合金与原合金的结构及电化学性能。XRD测试结果表明回收合金与原合金的结构相同，均为CaCu5型。恒电流充放电实验发现，回收合金与原合金粉相比，放电容量接近，放电电位高。不寿命测试结果表明，回收合金较原合金容量衰减更缓慢。     

在电场和磁场下手性侧链丙烯酸酯液晶聚合物的行为

在电场和磁场下手性侧链丙烯酸酯液晶聚合物的行为江雪平金顺子漆宗能张树范（中国科学院化学研究所北京１０００８０）关键词手性侧链丙烯酸酯液晶聚合物，红外二向色性，液晶分子排列含有手性液晶基团的聚合物，分子中含有不对称碳原子，在一定条件下使液晶聚合物...     

构建虚实结合、线上线下的制药工程专业实验项目——以湖北大学制药工程专业实验改革为例

To effectively solve the problem of insufficient engineering ability in the experimental teaching of pharmaceutical engineering major, the engineering integrated experimental device was designed and developed based on the idea of "one main body and two wings". On the basis of this device, the "virtual and real combination, online and offline" laboratory teaching project of production simulation and unit operation engineering simulation for anti-cough drug levodropropizine was implemented. It is expected to enhance students' engineering awareness and enhance the engineering ability to meet the new requirements of the "new engineering discipline" for professional laboratory teaching.     

手性丙烯酸酯侧链液晶聚合物液晶行为的聚合物效应

研究了手性丙烯酸酯侧链液晶聚合物聚４ 丙烯酰十一烷氧基 ４′ ［Ｓ（ ） ２ 甲基丁氧基］ 联苯（ＰＡ １１）的分子量对其液晶行为的影响，结果表明，ＰＡ １１的相变温度随聚合度增大而升高，当聚合度大于１５以后，分子量对相变温度影响不明显，低聚合度的ＰＡ １１只表现出双向性的ＳＡ相，而聚合度大于７的ＰＡ １１不仅具有双向性ＳＡ相，而且还表现出双向性ＳＣ相；分子量分布对ＰＡ １１的液晶相转变也有影响     

Nitrobenzene electrochemical sensor based on silver nanoparticle supported on poly-melamine functional multi-walled carbon nanotube

A novel and convenient electrochemical sensor, based on multi-walled carbon nanotube (MWCNT)–poly-melamine(PMel)–silver nanoparticle (AgNP) composite-modified glassy carbon electrode (GCE), was fabricated for the determination of nitrobenzene (NB). The modified electrode not only played an efficient electrocatalytic role for the reduction of NB but also significantly reduced the overpotential of NB, and the peak current increased greatly compared with bare GCE or other modified electrodes. The excellent performance of NB sensor can be ascribed to the synergistic effect between MWCNT and AgNP. The synergistic effect promoted the electron transfer between MWCNT and AgNP significantly and enhanced the electrochemical reduction ability of NB remarkably. Besides, PMel has huge nitrogen and amine groups, which contributes to the dispersion of silver nanoparticles and also improves the electrocatalytic activity and sensitivity of the sensor. The integration of PMel/MWCNT with AgNP provided a high-performance platform for the NB determination. Under the optimized experimental conditions, the developed sensor showed a wide linear calibration ranges from 20 to 1000 μM and from 1000 to 6000 μM, with a low detection limit (0.55 μM) for the detection of NB. At the same time, the modified electrode exhibited good stability and reproducibility and acceptable selectivity. Moreover, the proposed sensors were successfully employed to determine NB in real samples, and the recoveries were between 97.2 and 104.6 %.     

带斜楔相控声束偏转聚焦延时特性研究

带斜楔的相控阵超声检测广泛应用于低碳钢薄壁工件焊缝的检测,研究带斜楔的相控阵超声偏转聚焦检测延时法则和对应的声场将使检测可靠性进一步提高。本文利用费马原理探究了相控阵超声探头辐射声波至楔块中,在楔块-工件平面界面发生模式转换入射到工件中的横波聚焦时各阵元延时的计算方法,数值求解指定焦点时各阵元的延时,并利用计算得到的延时进行声场仿真和实验测量,发现声波能很好的聚焦在目标点,仿真和实验结果吻合。     

Evaluation of confidence and reproducibility in quantitative proteomics performed by a capillary isoelectric focusing-based proteomic platform coupled with a spectral counting approach

Multidimensional separations of the peptides resulting from enzymatic digestions of complex protein mixtures prior to MS/MS, namely shotgun proteomics, is increasingly utilized for large-scale identification and quantitation of proteins. Inherent to the performance of proteomic measurements is the resolving power of each of the separations both separately and in combination. By simply raising the number of CIEF fractions, the resulting enhancement in the overall peak capacity of combined CIEF/nano-RPLC separations greatly reduces the complexity of eluted peptides prior to MS detection and sequencing and increases the proteome coverage. The capabilities of the CIEF-based proteome platform coupled with the spectral counting approach to confidently and reproducibly quantify proteins and changes in protein expression levels among samples are evaluated. Analytical reproducibility of relative protein abundance is determined to exhibit a Pearson R(2) value greater than 0.99 and a CV of 14.1%. The platform is demonstrated to be capable of measuring changes in protein expression as low as 1.5-fold, with confidence following multiple testing adjustment.     

分析化学教学方法探索与实践

分析化学是化学及其相关专业的专业基础课程,内容丰富而繁杂,科学可行的教学方法是提高教学质量的保证。本文作者对多年来分析化学课程教学方法探索和实践进行了经验总结,包括重视第一节课教学,让学生对本课程"一见钟情";训练分析化学学科的微观思维;巧用形象比喻,加强对知识的理解和领悟;关注知识的层次和逻辑结构,突出重点知识;融合微观和宏观思维,快速掌握知识体系;展示分析化学学科之美;循环重复教学法,提高教学效果;立足课本且走出课本,扩大知识面和提高解决问题的能力等。