Microdetermination of proteins using m-carboxychlorophosphonazo as detection probe by enhanced resonance light scattering spectroscopy

Proteins can be determined using a common spectrofluorometer to detect the intensity of resonance light scattering (RLS). Under acidic conditions, the reaction between m-carboxychlorophosphonazo (CPA-mK) and proteins enhances the weak light scattering of CPA-mK drastically. This enhanced intensity is proportional to the concentration of proteins. The linear ranges for human serum albumin are 0.5-35.0 microg/mL, with detection limits of 0.104 microg/mL. The method yields results comparable to those of the calorimetric method using Coomassie Brilliant Blue G-250 (CBB) with relative standard deviations of 0.72-2.10% (n = 10). There is almost no interference by amino acids and most of the metal ions.     

钴铈复合金属氧化物催化剂上氧化亚氮催化分解性能研究

采用共沉淀法制备了一系列钴铈复合金属氧化物催化剂CoCex(x=0～0.2,x为Ce/Co摩尔比),进行了比表面积和XRD表征,并考察了它们对氧化亚氮催化分解反应的活性.结果表明,随着铈添加量的增加,催化剂上钴尖晶石相的衍射峰逐渐宽化,同时催化剂的比表面积逐渐增大;催化剂的活性随着铈添加量的增加先升高,然后下降,CoCe0.05催化剂表现出了最佳催化活性.在对CoCe0.05催化剂制备条件的考察实验中发现,共沉淀过程中控制pH值在9左右,于400 ℃焙烧得到的催化剂的活性最好.当反应气氛中存在O2或H2O时,CoCe0.05催化剂上N2O的分解反应受到抑制,但这种影响是可逆的,可能是由于它们与N2O在相同的活性位上存在竞争吸附.     

Two noncentrosymmetric complexes: [W(CO)4(bipy)] and [W(CO)4(phen)](bipy = 2,2′-bipyridine, phen = 1,10-phenanthroline) obtained through solvothermal synthesis and their optical properties

Solvothermal treatments of W(CO)₆ with 2,2′-bipyridine and 1,10-phenanthroline give [W(CO)₄(bipy)] (1) and [W(CO)₄(phen)] (2), respectively, which both crystallize in noncentrosymmetric space groups, suggesting that they meet the requirement of second harmonic generation (SHG) investigations. The preliminary experiment indicates that they are SHG active, and approximately estimated to be that of urea.     

介体型乳酸脱氢酶生物传感器的研制及应用

本报道了能对丙酮酸产生良好响应的电流型乳酸脱氢酶生物传感器。该传感器以耐尔兰Ａ修饰浸石墨电极为基体电极，将酶直接固化在蚕丝蛋白膜上。在ＰＨ７．４的ＮａＨ２ＰＯ４－ＮａＯＨ介质中，当２．４×１０＾－４ｍｏｌ／Ｌ的辅酶Ｉ（ＮＡＤＨ）存在下，该传感器的响应电流与丙酮酸浓度在３．２×１０＾－５ｍｏｌ／Ｌ范围内有良好的线性关系。响应时间为６０ｓ．本讨论了影响传感器响应的各种因素，用此传感器测定了人血清中     

巯基化合物的电位分析法研究

本文对用直接电位法测试巯基化合物的可行性进行了研究。发现由硫化银，硫化汞等金属难溶盐制成的膜电极对６－巯基嘌呤，高胱氨酸，１，４－二巯基苏糖经合物可产生响应，其斜率为３０－１１０ｍＶ／ｐＣ，电极响应时间，则为十几秒至十几分钏，测试结果具有较好的重现性。因此，该方法可望得到应用。文中还对有关响应机理进行了探讨。     

Solid-state synthesis and properties of SmCoO3

In this paper, perovskite oxide SmCoO₃ was prepared by the solid-state reaction method using Co₂O₃ and Sm₂O₃ as raw materials. The structure and properties of the samples were investigated by XRD, Raman spectral techniques, and DC measurements and so on. The results of XRD and Raman spectra showed that the mixtures of Co₂O₃ and Sm₂O₃ can react to produce a single phase perovskite oxide SmCoO₃ around 1353 K. The single-phase SmCoO₃ changes from an insulator to a semi-conductor and transition occurs around 470 K. The thermal expansion coefficient (2.17 × 10⁻⁵ K⁻¹) of the single-phase SmCoO₃ is approximately equal to that of doped LaGaO₃, but much bigger than that of SDC(Ce_0.85Sm_0.15O₂) above 873 K.     

2,6—双（1′—苯基—3′—甲基—5′—氧代—4′—甲酰吡唑）吡啶稀土配合物的合成

在乙醇－水溶液中合成了１４种２，６－双（１′－苯基－３′－甲基－５′－氧代－４′－甲酰吡啶）吡啶稀土配合物，元素分析确定其化学组成的ＲＥ２Ｌ３．ｎＨ２Ｏ并用紫外可见光谱、红外光谱，核磁共振谱，热分析，荧光光谱，摩尔电导等进行了表征。     

PEO／PVP共混体系结晶行为

本文用WAXD、PLM、DSC方法研究了聚氧化乙烯(PEO)/聚乙烯基吡咯烷酮(PVP)共混体系的结晶行为,探索了两组分聚合物间相互作用及体系结晶度与非晶组分含量的关系。DSC研究表明PEO/PVP共混体系具有两个玻璃化转变温度,分别是纯组分的T_g,无相容性。应用Avrami和LH方程对其动力学参数进行了研究。偏光显微镜观察了共混物结构形态。     

无烟煤氧化制苯多羧酸

对阳泉无烟煤进行了碱－氧气氧化研究。发现反应温度、碱的用量、反应时间以及氧初压是影响和控制水溶酸产率和反应速度的重要因素。最佳氧化条件为：温度２６０℃，碱煤比３．２８：１，反应时间２ｈ，氧初压５．５ＭＰａ。在最佳氧化条件下水溶酸的产率高达６６．０％，其中６７．４％为苯多羧酸。并采用红外光谱和气相色谱对反应产物进行了定性和定量分析。