Peptide Dimethylation: Fragmentation Control via Distancing the Dimethylamino Group

Direct reductive methylation of peptides is a common method for quantitative proteomics. It is an active derivatization technique; with participation of the dimethylamino group, the derivatized peptides preferentially release intense a₁ ions. The advantageous generation of a₁ ions for quantitative proteomic profiling, however, is not desirable for targeted proteomic quantitation using multiple reaction monitoring mass spectrometry; this mass spectrometric method prefers the derivatizing group to stay with the intact peptide ions and multiple fragments as passive mass tags. This work investigated collisional fragmentation of peptides whose amine groups were derivatized with five linear ω-dimethylamino acids, from 2-(dimethylamino)-acetic acid to 6-(dimethylamino)-hexanoic acid. Tandem mass spectra of the derivatized tryptic peptides revealed different preferential breakdown pathways. Together with energy resolved mass spectrometry, it was found that shutting down the active participation of the terminal dimethylamino group in fragmentation of derivatized peptides is possible. However, it took a separation of five methylene groups between the terminal dimethylamino group and the amide formed upon peptide derivatization. For the first time, the gas-phase fragmentation of peptides derivatized with linear ω-dimethylamino acids of systematically increasing alkyl chain lengths is reported. Figure

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一氧化碳/苯乙烯共聚催化体系的优化

用醋酸钯,１,１０－邻菲咯啉,对甲苯磺酸,对苯二醌和甲醇组成的催化体系进行了一氧化碳／苯乙烯共聚反应,成功地制得了一氧化碳和苯乙烯的线性交替共聚物－聚酮（ＳＴＣＯ０,在催化体系组成的筛选过程中,对人工神经网络的应用进行了探索性研究。首先,采用ＶｉｓｕａｌＣ＋＋１．５２编制了ＢＰ神经网络,利用正交实验结果对该神经网络进行了训练,训练误差小于５％。     

The amplified model of erbium-doped fiber amplifier (EDFA) with cross-phase modulation (XPM)

Based on the rate equations and the power propagation equations, the amplified model is obtained which considers the cross-phase modulation (XPM)-induced intensity fluctuation between pump power and signal power in erbium-doped fiber amplifier (EDFA). Simulation results show: a higher nonlinear coefficient and a stronger pump power cause a stronger XPM in EDFA. Before the optimal length of EDF, the intensity caused by XPM increases with the increasing length, but after the optimal length, the XPM presents saturation. A bigger nonlinear coefficient γ corresponds to a smaller optimal length and gain, and makes gain saturation more easy. XPM effect will decrease EDFA’s amplified efficiency.     

矩阵方程AXB=D的最小二乘Hermite解及其加权最佳逼近

本中,我们讨论了矩阵方程AXB=D的最小二乘Hermite解,通过运用广义奇异值分解(GSVD)，获得了解的通式。此外,对于给定矩阵F，也得到了它的加权最佳逼近表达式。     

厚胶光刻非线性畸变的校正

利用厚胶光刻技术制作大深度微结构元件是一种有效的途径,但厚胶光刻过程中的非线性畸变对光刻面形质量的严重影响限制了该技术的应用,基于此,提出了一种对掩模透射率函数进行校正的方法。分析空间像形成及其在光刻胶内传递、曝光、显影等过程中非线性因素的影响,利用模拟退火算法对掩模透射率函数进行校正,以提高光刻面形质量,并以凹面柱透镜为例,给出了校正前后的显影轮廓模拟结果,其校正后浮雕面形的体积偏差仅为2.63%。该方法在有效改善面形质量的同时,并没有引起掩模的设计、制作难度及费用增加,这对于设计、制作高质量的微结构元件有重要意义。     

Comparing ordered-entry queues with heterogeneous servers

We study a queueing system withm exponential servers with distinct service rates. Jobs arrive at the system following an arbitrary point process. Arrived jobs receive service at the first unoccupied server (if any) according to an entry order , which is a permutation of the integers 1, 2,...,m. The system has a finite buffer capacity. When the buffer limit is reached, arrivals will be blocked. Blocked jobs will either be lost or come back as New arrivals after a random travel time. We are concerned with the dynamic stochastic behavior of the system under different entry orders. A partial ordering is established among entry orders, and is shown to result in some quite strong orderings among the associated stochastic processes that reflect the congestion and the service characteristics of the system. The results developed here complement existing comparison results for queues with homogeneous servers, and can be applied to aid the design of conveyor and communication systems.     

方程w"-w+f(t,w)=O的Dirichlet边值问题的正解存在性与多解性

考察了下列常微分方程的Dirichlet边值问题的正解[w″(t)-w(t) f(t,w(t))=0,0≤t≤1 w(0)=w(1)=0建立了n正解的存在性，其中n是一个任意的自然数。     

LD端面抽运Nd:YAG 1319nm/1338nm双波长激光器研究

从LD端面抽运固体激光器的激光阈值公式出发，建立了双波长激光同时振荡的阈值条件，理论计算了腔镜对于两个波长的透过率关系，实现了LD端面抽运Nd:YAG 1319nm/1338nm双波长激光连续和准连续输出.双波长激光连续输出功率可达6W，斜效率为30%；准连续输出功率在重复频率50kHz时可达4.75W，斜效率为24.73%，脉冲宽度为55.05ns；腔内插入布儒斯特片，在重复频率为50kHz时，双波长激光准连续线偏振输出功率可达2.22W，不稳定性小于0.52%，M²     

化学拆分的新方法研究

主要介绍了近年来发展起来的两种化学拆分新方法,包括拆分和组合拆分;并简要论述了化学拆分中的手性识别现象,以及化学拆分方法在手性药物制备中的应用。     

HIAF高能辐照终端感生放射性

本工作是基于蒙特卡罗模拟软件FLUKA对高能强流重离子加速器（HIAF）高能辐照终端感生放射性进行初步研究。该终端可运行质子最高能量为9.3 GeV,最大流强是1.45×1012 pps（particle per second）。研究内容包括:（1）预测高能辐照终端内活化物质的放射性活度特性;（2）预测不同冷却时间高能辐照终端内残余剂量率分布。研究结果表明,HIAF正常运行时高能辐照终端内的感生放射性主要受束流垃圾桶活化产生的放射性核素影响。当加速器连续运行100天冷却4小时,垃圾桶表面残余剂量率为2.375 mSv·h-1。终端内空气中13N和15O动态饱和比浓度大于其对应的导出空气浓度。冷却水中13N和15O的活度大于对应的ALI_min。该研究是HIAF辐射防护基础研究以及加速器环境影响评价的一项重要内容。The Monte Carlo code FLUKA was used to predict the induced radioactivity of high-energy irradiation terminal of HIAF. The maximum energy of proton is 9.3 GeV, and the maximum current is 1.45×1012 pps (particle per second). In this study we were to predict:(1) the activity properties of activated substances in the experimental terminal; (2) the residual dose rate distribution in the experimental terminal at different cooling time. The results indicate that the induced radioactivity in the high energy irradiation terminal of the HIAF is mainly affected by the radionuclide induced in the beam dump. The residual dose rate on the surface of the beam dump is 2.375 mSv·h-1, after 100 d irradiation and 4 h cooling. The dynamic saturation ratio of 13N and 15O induced in the air inside the terminal is higher than its corresponding derived air concentration. The activity of 13 N and15O induced in cooling water is higher than its ALI_min. This study is a part of radiation protection basic research and environmental impact assessment for HIAF.