Comparison and optimization of strategies for a more profound profiling of the sialylated N-glycoproteomics in human plasma using metal oxide enrichment

Glycosylation is an important posttranslational modification of proteins and plays a crucial role in both cellular functions and secretory pathways. Sialic acids (SAs), a family of nine-carbon-containing acidic monosaccharides, often terminate the glycan structures of cell surface molecules and secreted glycoproteins and perform an important role in many biological processes. Hence, a more profound profiling of the sialylated glycoproteomics may improve our knowledge of this modification and its effects on protein functions. Here, we systematically investigated different strategies to enrich the SA proteins in human plasma using a newly developed technology that utilizes titanium dioxide for sialylated N-glycoproteomics profiling by mass spectrometry. Our results showed that using a combination of a filter-aided sample preparation method, TiO₂ chromatography, multiple enzyme digestion, and two-dimensional reversed-phase peptide fractionation led to a more profound profiling of the SA proteome. In total, 982 glycosylation sites in 413 proteins were identified, among which 37.8 % were newly identified, to establish the largest database of sialic acid containing proteins from human plasma.

Single-molecule monitoring in living cells by use of fluorescence microscopy

Monitoring single molecules in living cells is becoming a powerful tool for study of the location, dynamics, and kinetics of individual biomolecules in real time. In recent decades, several optical imaging techniques, for example epi-fluorescence microscopy, total internal reflection fluorescence microscopy (TIRFM), confocal microscopy, quasi-TIRFM, and single-point edge excitation subdiffraction microscopy (SPEED), have been developed, and their capability of capturing single-molecule dynamics in living cells has been demonstrated. In this review, we briefly summarize recent advances in the use of these imaging techniques for monitoring single-molecules in living cells for a better understanding of important biological processes, and discuss future developments.     

用于光孤子通信的理想分布式放大器

光孤子通讯的一个重要的问题是如何提高光孤子通讯的站间距离，降低线路成本，本文从降低信号在线路中的起伏入手，通过理论分析和计算机模拟，提出了用于孤子通讯的理想分布式光纤放大器的设想，借以降低噪声和提高泵浦站间距，降低线路成本，并对一些设计参数对系统的影响进行了讨论。     

胶束增敏导数分光光度法测定微量铜

本文研究了在非离子型胶束Ｔｒｉｔｏｎ Ｘ－１００体系中，以１（２－吡啶偶氮）２－萘酚为显色剂，用二阶导数分光光度法消除镉的干扰，与乙醇介质比较，测定灵敏度提高，测定镉样中微量铜，结果较为满意。     

Shape co-existence and structural evolution of thevi13/2 band in157Yb

The high-spin states of¹⁵⁷Yb have been studied via the reaction of¹⁴⁴Sm(¹⁶O, 3n) at¹⁶O energy of 90 MeV using techniques of in-beam γ-ray spectroscopy. Measurement of γ-γ-t coincidences was performed with 11 BGO(AC)HPGe detectors. Based on the γ-γ coincidence relationships and the measured results of γ-ray anisotropies and DCO ratios, the level scheme for¹⁵⁷Yb was established. The shape co-existence and structural evolution of thevi_13/2 band with increasing angular momentum in¹⁵⁷Yb have been discussed. The systematics of thevi_13/2 bands in theN=87 odd-A isotones have been compared.     

外腔式可调谐半导体激光器的光谱法求解

利用由射线法求得的光谱表达式，分析了外腔式半导体激光器的输出谱，并以此求得了普遍情况下阈值载流子密度的解析表达式，结合光谱表达式与载流子速率方程，求得了不同电流下腔内载流子密度与阈值的差值，从而可以不必诸光子数速度方程而获得外腔式半导体激光器的自洽解。     

ABCD矩阵方法在主动锁模光纤激光器理论与实验分析中的应用

应用ABCD矩阵方法从理论上对主动锁模环形光纤激光器进行了分析并与实验结果进行了比较.