One-PCR-tube approach for in situ DNA isolation and detection

Traditional real-time polymerase chain reaction (PCR) requires a purified DNA sample for PCR amplification and detection. This requires PCR tests be conducted in clean laboratories, and limits its applications for field tests. This work developed a method that can carry out DNA purification, amplification and detection in a single PCR tube. The polypropylene PCR tube was first treated with chromic acid and peptide nucleic acids (PNA) as DNA-capturer were immobilized on the internal surface of the tube. Cauliflower mosaic virus 35S (CaMV-35S) promoter in the crude extract was hybridized with the PNA on the tube surface, and the inhibitors, interfering agents and irrelevant DNA in the crude extract were effectively removed by rinsing with buffer solutions. The tube that has captured the target DNA can be used for the following real-time PCR (RT-PCR). By using this approach, the detection of less than 2500 copies of 35S plasmids in a complex sample could be completed within 3 hours. Chocolate samples were tested for real sample analysis, and 35S plasmids in genetically modified chocolate samples have been successfully identified with this method in situ. The novel One-PCR-tube method is competitive for commercial kits with the same time and simpler operation procedure. This method may be widely used for identifying food that contains modified DNA and specific pathogens in the field.     

Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma

A strategy for the determination of the presence of thiol-containing amino acids was successfully established by simply assembling copper chloride and xylenol orange (3,3'-bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonephthalein trisodium salt; XO) in a 1 : 1 molar ratio in quasi-physiological water solution (pH 6.0). The copper(II)-XO ensemble was highly selective for thiol species such as cysteine, homocysteine, and glutathione without interference from other amino acids and could quantitatively detect thiol in the range from 10 to 200 μM with a linear relationship having an average molar absorbance constant of 6530 L mol(-1) cm(-1) in pure water. The whole recognition process for thiol gave rise to a rapid visual color change from purple-red to yellow which can be observed simultaneously with the naked-eye.     

一种基于光谱分析的二元不完全重叠色谱峰的分离解析方法

基于二极管阵列检测器获得的色谱-光谱数据,建立了一种二元不完全重叠液相色谱峰的解析方法: 色谱数据经过去噪、归一化处理后,计算各时间点的光谱差异并进行系统聚类分析,提取特征光谱后,利用非负最小二乘法对色谱-光谱矩阵进行解析,得到基于特征光谱的流出曲线,进而得到分离后的色谱峰。将解析结果和纯标样的色谱峰进行比较,解析后的光谱图和纯标样的光谱图无显著差异,保留时间相差小于0.01 min。实验结果表明,该方法在二元不完全重叠液相色谱峰的解析方面能取得良好的效果。     

Synthesis and characterization of novel conjugated polymers based on 3-cyclobutene-1,2-dione moiety

Three novel conjugated polymers bearing 3,4-bis（4-hexylthiophen-2-yl）-3-cyclobutene-1,2-dione unit in their main chain have been synthesized successfully in good yields through Suzuki or Stille coupling reaction.Their molecular structures have been confirmed by FT-IR,¹H NMR and ¹³C NMR.All these copolymers exhibit broad and strong absorption bands in UV-vis region,and their optical band gaps are calculated to be 1.6-2.0 eV.suggesting that they have good coverage with the solar spectrum.These polymers have good thermostability and solubility in common organic solvents.Moreover,all these objective macromolecules possess high electron affinity of～3.8 eV determined from cyclic voltammetry measurement,implying that they are potential n-type polymeric photovoltaic materials.     

Direct ab initio dynamics study of radical C4H (X̃2Σ+) + CH4 reaction

The methane (CH(4)) hydrogen abstraction reaction by linear butadiynyl radical C(4)H (CCCCH) has been investigated by direct ab initio dynamics over a wide temperature range of 100-3000 K, theoretically. The potential energy surfaces (PESs) have been constructed at the CCSD(T)/aug-cc-pVTZ//BB1K/6-311G(d,p) levels of theory. Two different hydrogen abstraction channels by C(1) and C(4) of C(4)H (C(1)C(2)C(3)C(4)H) have been considered. The results indicate that the C(1) position of C(4)H is a more reactive site. The electron transfer behaviors of two possible channels are also analyzed by quasi-restricted orbital (QRO) in detail. The rate constants calculated by canonical variational transition-state theory (CVT) with the small-curvature tunneling correction (SCT) are in excellent agreement with available experimental values. The normal and three-parameter expressions of Arrhenius rate constants are also provided within 100-3000 K. It is expected to be helpful for further studies on the reaction dynamics behaviors over a wide temperature range where no experimental data is available so far.     

Preparation of hybrid mesoporous silica luminescent nanoparticles with lanthanide(III) complexes and their exhibition of white emission

We chose dipicolinic acid as a tridentate chelating unit featuring ONO donors to react with lanthanide(III) ions to yield tight and protective N(3)O(6) environments around the lanthanide(III) ions. We immobilized the lanthanide(III)-dipicolinic acid complexes on colloidal mesoporous silica with diameter smaller than 100 nm by a covalent bond grafting technique and obtained nearly monodisperse luminescent Eu-dpa-Si and Tb-dpa-Si functionalized hybrid mesoporous silica nanomaterials. These hybrid nanomaterials were characterized by powder X-ray diffraction, scanning electron microscopy, transmission electron microscopy, thermogravimetric analysis, nitrogen adsorption-desorption, and photoluminescence spectroscopic techniques. The hybrid mesoporous silica nanoparticles exhibit intense emission lines upon UV-light irradiation, owing to the effective intramolecular energy transfer from the chromophore to the central lanthanide Eu(3+) and Tb(3+) ions. Furthermore, the functionalized nanomaterials can be turned to white light materials after annealing at high temperature.     

高能重离子碰撞中末态粒子分布的非对称性

对Bevalac1.2A GeV Au+Au碰撞实验,根据以流方向为z轴的质心坐标系中末态粒子方位角分布的非对称性为极小的条件,确定了事件中五种质量范围(A=1,2,3,4,5-8)末态粒子的集合侧向流流角.在以流方向为z轴的质心坐标系中研究了不同质量末态粒子极角分布,结果表明质量越大的粒子在流方向分布得越集中,侧向流的“流”值越大.     

Laser diode pumped LNYAB self-frequency-doubling lasers

The LNYAB self-doubling laser pumped by (LD) has been developed for the first time. The properties of the lasers are much better than those of NYAB self-doubling lasers pumped by LD. The LNYAB self-doubling laser pumped by LD can be operated in TEM_(00) mode with threshold pumping power of 3.6mW which is lower than that of LD pumped NYAB self-doubling lasers by 74%. The output power is 29mW at 0.531μm in green region with optical-to-optical efficiency of 4.8% which is higher than that of NYAB self-doubling laser by 20%.     

Preparations on Several GRIN Lenses

Gradient index materials have been developed from glass melting process to GRIN lenses. Two kinds of GRIN lenses have been fabricated by ion-exchange and glass fusion/diffusion. The main applications to GRIN lenses are demonstrated.     

一种五波长半导体激光生物组织辐照仪

采用GaAlAs单量子阱半导体激光器和LD泵浦的Nd:YVO4－KTP倍频激光器等研制了国内首台可从光导纤维输出5种波长的半导体激光生物组织辐照仪,该仪器已在激光生物医学领域投入应用．