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101.
The fluorescence lifetime strongly depends on the immediate environment of the fluorophore. Time-resolved fluorescence measurements of the enhanced forms of ECFP and EYFP in water–glycerol mixtures were performed to quantify the effects of the refractive index and viscosity on the fluorescence lifetimes of these proteins. The experimental data show for ECFP and EYFP two fluorescence lifetime components: one short lifetime of about 1 ns and a longer lifetime of about 3.7 ns of ECFP and for EYFP 3.4. The fluorescence of ECFP is very heterogeneous, which can be explained by the presence of two populations: a conformation (67% present) where the fluorophore is less quenched than in the other conformation (33% present). The fluorescence decay of EYFP is much more homogeneous and the amplitude of the short fluorescence lifetime is about 5%. The fluorescence anisotropy decays show that the rotational correlation time of both proteins scales with increasing viscosity of the solvent similarly as shown earlier for GFP. The rotational correlation times are identical for ECFP and EYFP, which can be expected since both proteins have the same shape and size. The only difference observed is the slightly lower initial anisotropy for ECFP as compared to the one of EYFP. 相似文献
102.
Importance sampling Monte Carlo offers powerful approaches to approximating Bayesian updating in sequential problems. Specific classes of such approaches are known as particle filters. These procedures rely on the simulation of samples or ensembles of the unknown quantities and the calculation of associated weights for the ensemble members. As time evolves and/or when applied in high-dimensional settings, such as those of interest in many data assimilation problems, these weights typically display undesirable features. The key difficulty involves a collapse toward approximate distributions concentrating virtually all of their probability on an implausibly few ensemble members.
After reviewing ensembling, Monte Carlo, importance sampling and particle filters, we present some approximations intended to moderate the problem of collapsing weights. The motivations for these suggestions are combinations of (i) the idea that key dynamical behavior in many systems actually takes place on a low dimensional manifold, and (ii) notions of statistical dimension reduction. We illustrate our suggestions in a problem of inference for ocean surface winds and atmospheric pressure. Real observational data are used. 相似文献
103.
Reflections from geometric discontinuities can be used with ultrasonic energy to predict the temperature of an interface where classical temperature measurement techniques are impractical because of physical access limitations or harsh environmental conditions. Additionally, these same ultrasonic measurements can be used with inversion methods commonly applied to ill-posed heat transfer problems to increase the accuracy of the measurement of surface temperature or heat flux at the surface of interest. Both methods for determining surface temperature are presented, along with a comparison of results both from a verification example and using data gathered in a field test of the methods. The results obtained with these two methods are shown to be in good agreement with an empirical relationship used in the design of large caliber guns. 相似文献
104.
Seweryniak D Woods PJ Carpenter MP Davinson T Janssens RV Jenkins DG Lauritsen T Lister CJ Ruiz C Shergur J Sinha S Woehr A 《Physical review letters》2005,94(3):032501
The level structure of (22)Mg has been studied with high-sensitivity gamma-ray spectroscopy techniques. A complete level scheme is derived incorporating all subthreshold states and all levels in the energy region relevant for novae burning. The excitation energy of the most important astrophysical resonance is measured with improved accuracy and found to differ from previous values. Combining the present result with a recent resonance energy measurement of this state leads to a derived (22)Mg mass excess of -400.5(13) keV. 相似文献
105.
Blagovest Bahnev Mark D. Bowden Agnieszka Stypczyńska Sylwia Ptasińska Nigel J. Mason Nicholas St.J. Braithwaite 《The European Physical Journal D - Atomic, Molecular, Optical and Plasma Physics》2014,68(6):1-5
The coupled-channel optical method (CCOM) is implemented in this work to study the scattering of electron on rubidium atom at 20 eV. In order to provide a realistic calculation, the continuum effect of the scattering system is accounted by incorporate an ab initio optical potential into the CCOM calculation. The differential cross sections (DCS), as well as the reduced Stokes parameters of 5p excitation are reported and compared to the available experimental and theoretical data. 相似文献
106.
Overmodulation of electron paramagnetic resonance (EPR) lines is routinely used in EPR oximetry in order to increase the signal-to-noise
ratio and thus to improve the accuracy with which the line width of a spin probe can be measured. For a known probe type,
the line width is easily translated into the oxygen partial pressure. A standard EPR spectrometer uses the analog phase-sensitive
detection (PSD) to demodulate the EPR signal. PSD imposes the restriction that only one spectrum is measured at a time, which
is normally the first-harmonic EPR line. Information about EPR signals centered at the other harmonics of the modulation frequency
is irreversibly destroyed by PSD. The question is raised whether this information can be utilized for EPR oximetry, for overmodulation
enhances the second- and the other harmonic spectra, so that they approach the first-harmonic spectrum in intensity. To find
an answer, numerical simulation and experimental measurements have been conducted. The experiment required modification of
the detection scheme, so that all EPR-related information in the overmodulated signal is preserved. This permits measuring
of the multiharmonic EPR spectrum, which when fitted to a set of the corresponding theoretical lines produces more accurate
results in comparison with the standard overmodulation method. 相似文献
107.
Mark Israelit 《General Relativity and Gravitation》2009,41(12):2847-2866
In the present work a procedure is build up, that allows obtaining dark matter (DM) and cosmic acceleration in our 4D universe
embedded in a 5D manifold. Both, DM and the factor causing cosmic acceleration, as well ordinary matter are induced in the
4D space-time by a warped, but empty from matter, 5D bulk. The procedure is carried out in the framework of the Weyl–Dirac
version (Israelit, Found Phys 35:1725, 2005; Israelit, Found Phys 35:1769, 2005) of Paul Wesson’s Induced Matter Theory (Wesson,
Space-time matter, 1999) enriched by Rosen’s approach (Found Phys 12:213, 1982). Considering chaotically oriented Weyl vector
fields, which exist in microscopic cells, we obtain cold dark matter (CDM) consisting of weylons, massive bosons having spin
1. Assuming homogeneity and isotropy at large scale we derive cosmological equations in which luminous matter, CDM and dark
energy may be considered separately. Making in the given procedure use of present observational data one can develop a model
of the Universe with conventional matter, DM and cosmic acceleration, induced by the 5D bulk. 相似文献
108.
Rolf MP Hofman MB Kuijer JP Pai VM Greiser A van Rossum AC Heethaar RM 《Magnetic resonance imaging》2009,27(3):385-392
Multiecho phase-contrast steady-state free precession (PC-SSFP) is a recently introduced sequence for flow quantification. In this multiecho approach, a phase reference and a velocity-encoded readout were acquired at different echo times after a single excitation. In this study, the sequence is validated in vitro for stationary flow. Subsequently, the sequence was evaluated on cardiac output measurements in vivo for through-plane flow in comparison to regular single gradient echo velocity quantification [phase-contrast spoiled gradient echo (PC-GE)]. In vitro results agreed with regular flow meters (RMS 0.1 cm/s). Cardiac output measurements with multiecho PC-SSFP on 10 healthy subjects gave on average the same results as the standard PC-GE. However, the limits of repeatability of PC-SSFP were significantly larger than those of PC-GE (2 l/min and 0.5 l/min, respectively, P=.001). The multiecho approach introduced some specific problems in vivo. The difference in echo times made the velocity maps sensitive for water-fat shifts and B(0)-drifts, which in turn made velocity offset correction problematic. Also, the addition of a single bipolar gradient cancelled the flow compensated nature of the SSFP sequence. In combination with the prolonged TR, this resulted in flow artifacts caused by high and pulsatile through-plane flow, affecting repeatability. Given the significantly lower repeatability of PC-SSFP, cardiac output in turn is less reliable, thus impairing the use of multiecho PC-SSFP. 相似文献
109.
Chakraborty S Mukherjee J Manna M Ghosh P Das S Denys MB 《Ultrasonics sonochemistry》2012,19(5):1044-1050
Nanoparticles, when homogeneously dispersed in a base fluid, e.g. water, ethylene glycol etc. are commonly known as nanofluids. Nanofluids have gained attention in the scientific community for their enhanced thermal properties. One of the major problems in using nanofluids as a heat transfer medium for commercial applications is that, in most of the closed circuit industrial cooling processes, the cooling fluid has to be replaced after several cycles of cooling operation because of an increased presence of contaminants. If nanofluids were used as a coolant, it would be very hard to separate the nanoparticles from the waste fluid. The present work is aimed at the separation and recycling of nanoparticles from fluid waste by means of quick settling of titanium dioxide nanoparticles using silver nanoparticles along with ultrasonic treatment. It is observed that with increasing silver concentration and time of ultrasonication, the stability of the dispersion decreases. There is a value for both the silver concentration and ultrasonication time above which the settling time decreases drastically. 相似文献
110.
We report the characteristics of current induced frequency modulation (FM) for two continuous-wave quantum cascade lasers (QCLs) at 9.06 μm. Both the frequency tuning rate and the phase shift between intensity modulation and FM are measured at different modulation frequencies from 10 Hz to 200 kHz. The frequency tuning rate of the QCLs depends on both the modulation frequency and amplitude. The tested QCL has been used to detect ambient water vapor with wavelength modulation spectroscopy for validation with a numerical model. 相似文献