Horizontal two-dimensional polyacrylamide gel electrophoresis with immobilised pH gradients in the first dimension has been applied to the analysis of human skin fibroblast and muscle myoblast total cell proteins. Excellent two-dimensional separations of skin fibroblast proteins were obtained using pH 4-10 immobilised pH gradient gels with a long interelectrode distance (16 cm), but resolution was degraded, particularly of the more acidic proteins, by the use of shorter (10 cm) gels. Improved resolution of acidic and basic proteins was obtained using separate pH 4-7 and pH 7-10 immobilised pH gradient gels respectively in the first dimension. Two-dimensional protein maps of skin fibroblast proteins were visualised both by silver staining and by autoradiography of samples labelled synthetically with [35S]methionine. Horizontal two-dimensional electrophoresis, using pH 4-7 and pH 7-10 immobilised pH gradient gels in the first dimension, was applied to the analysis of protein samples from skin fibroblasts and muscle myoblasts dual-labelled synthetically with [35S]methionine and [75Se]selenomethionine in an attempt to identify sets of proteins specific to each cell type. In addition, two-dimensional maps or protein samples derived from normal individuals and patients with Duchenne muscular dystrophy were compared to search for protein changes associated with the disease state. Although sets of qualitative protein spot differences were observed by visual inspection of the two-dimensional gels, more rigorous qualitative and quantitative analysis of the patterns using a computerised analysis system will be required to obtain the maximum amount of information from these data. 相似文献
Condensation of pyridoxal with the methyl ester of glycine gave the aminocoumarin analog 3-amino-8-rnethyl-2-oxo-2H-pyrano[2,3-c]pyridine-5-methanol (III), and not the expected aldimine. The structure of the compound was deduced by independent synthesis and by comparing its NMR spectrum with that of 3-aminocoumarin. The new reaction provides routes for the synthesis of α4-pyridoxylideneglycine and α4-pyridoxyloxoacetic acid derivatives. It may also provide a model for irreversible inactivation of vitamin B6 in biochemical systems. 相似文献
Triphenylphosphane Nickel(0) Complexes with Isocyanide Ligands — [(RNC)nNi(PPh3)4–n] (n = 1–3) Synthesis and properties of the isocyanide triphenylphosphane nickel(0) complexes [(RNC)Ni(PPh3)3], [(RNC)2Ni(PPh3)2] and [(RNC)3Ni(PPh3)] (R = tBu, Cy, PhCH2, p-TosCH2) are described. I.r. and 31P n.m.r. spectra were recorded and the X-ray crystal structure of [(PhCH2NC)2Ni(PPh3)2] was determined. 相似文献
The mass spectra of several dibenzo (d.f)-1,3,2,-dioxaphosphorepines of the type where X=0, S, Se and R=2,2′-biphenylylene, have been studied. Dibenzochalcogen ions [RX]+˙ stemming directly from [M]+˙ were observed with high intensities, indicating specific migration of X from phosphorus to carbon with simultaneous destruction of the dioxaphosphorepine ring. Simple cleavage of P? Y as usually observed in similar organophosphorus compounds is not a common process here. 相似文献
Zusammenfassung Na-cyklohexyldithiocarbaminat reagiert im pH-Bereich 6,6 bis 7,2 mit Uranylsalzen unter Bildung eines in Wasser mit gelber Farbe lösliehen Komplexes, der bei pH 6,6 ein Absorptionsmaximum bei 392 bis 394 nm besitzt. Die wäßrige Lösung wird durch Zusatz von Alkalilauge bis zum pH 8 bis 9 momentan entfärbt.Es wird ein Kaliumdoppelsalz KUO2(S2HCNC6H11)3 · 3 H2O beschrieben; ferner wird über die Umwandlung des Calciumdoppelsalzes beim Kochen mit NaOH berichtet.
Summary Sodium cyolohexyldithiooarbamate reacts, in the pH range 6.6 to 7.2, with uranium salts to yield a complex which gives a yellow aqueous solution. The absorption maximum is at 392–394 nm at pH 6.6. The aqueous solution loses its color immediately when caustic alkali is added to raise the pH to 8 or 9.A potassium double salt, KUO2(S2HCNC6H11)3 · 3 H2O is described. A report is also given of the transformation of the calcium double salt on boiling with NaOH.
Résumé Le dithiocarbamate de cyclohexylsodium réagit dans le domaine de pH 6,6 à 7,2 sur les sels d'uranyle avec formation d'un complexe coloré en jaune, soluble dans l'eau, qui possède à pH 6,6 un maximum d'absorption pour 392–394 nm. Par addition de lessive alcaline jusqu'à pH 8 à 9 on décolore momentanément la solution aqueuse.On décrit un sel double de potassium KUO2(S2HCNC6H11)3 · 3 H2O; on rend compte, de plus, de la transposition du sel double de calcium par chauffage avec la soude.
Gewidmet zur 100. Wiederkehr des Geburtstages vonFriedrich Emich. 相似文献
1. Historical remarks concerning magnetic influence on the polarization of resonance fluorescence and its explanation as the Zeeman effect. 2. Rotation of the plane of polarization of the resonance fluorescence of Hg by weak magnetic fields and determination of the lifetime of the excited state. 3. Experiments concerning the excitation of the resonance fluorescence of Hg by light with different kinds and directions of polarization. 相似文献
Zusammenfassung Ursprung und Entwicklung der dokimatistischen Analyse sowie die alten Verfahren der Gold-Silber-Trennung werden geschildert. Anschließend werden die klassische Lötrohranalyse sowie die Flammenreaktionen von Bunsen behandelt. Zum Schluß sind die sich auf diese alten Verfahren stützenden Fortentwicklungen der klassischen Probiermethoden erwähnt, die zu den leistungsfähigsten Bestimmungsverfahren der neuzeitlichen Analyse zu rechnen sind, wie die Bestimmung kleiner Hg-Mengen nach Stock, die mikrodokimastischen Verfahren von Haber u. Mitarb. sowie die Verdampfungsanalyse in ihren verschiedenen Anwendungen. 相似文献
In an investigation of octamethylene bis(malonic acid diamides) and their selectivities for magnesium, it was found that presence of secondary amides within the particular ionophore played a considerable role in the enhancement of magnesium selectivity. Similar effects in other ionophores, i.e. tris(malonic acid diamides), were thus systematically looked at with the help of selectivity measurements with the hope of optimizing the number of secondary and tertiary amides so as to improve the magnesium selectivity. The syntheses of several investigated tris(malonic acid diamide) isologues are equally reported.Deceased in November 1992 相似文献
The di- and tetra-allyl ethers of tert-butylcalix[4]arene 1 and 2 have been prepared by alkylation of tert-butylcalix[4]arene with allyl bromide and K2CO3 using different reaction times. Solution 1H NMR measurement of the di-allyl ether 1 and X-ray crystal structures of the complexes of 1 with chloroform (1a) or methanol (1b) indicate the cone conformation of 1 in which intramolecular hydrogen bonding can be maximized. The crystalline state conformers 1a and 1b are distorted in different grades depending on the solvent. While methanol is incorporated in the macrocycle, chloroform molecules do not occupy the cage. The solution 1H NMR spectra of tetra-allyl ether 2 show the co-existence of the cone and partial cone conformation. The partial cone conformer of 2 was investigated by X-ray crystallography. In this compound hydrogen bonding is not existent. The conformer distribution is likely affected by steric and template effects. 相似文献