Study of a railgun plasma armature

An experimental and theoretical study of a railgun plasma is presented. The experimental results have been obtained by recording the voltage and current variations as well as the spectral emission of the plasma. The gas pressure, helium, or air in contact with the exploding foil has been varied from 0.1 to 2 MPa. The apparatus consisted of a test chamber simulating a railgun facility. To complete the study, some measurements of the plasma characteristics in the 500-kJ EMA 1 railgun system have been recorded. It is shown that the resistance of the plasma, which is 15 mΩ at 10 kA, decreases to 0.2 mΩ at 500 kA. These results indicate the effect of the energy flow to the walls and that of the ionization potential of the gas in contact with the exploding foil at lower currents     

Analysis of codeine, dihydrocodeine and their glucuronides in human urine by electrokinetic capillary immunoassays and capillary electrophoresis-ion trap mass spectrometry

Screening for and confirmation of illicit, abused and banned drugs in human urine is a timely topic in which capillary separation techniques play a key role. Capillary electrophoresis (CE) represents the newest technology employed in this field of analysis. Two rapid competitive binding, electrokinetic capillary-based immunoassays are shown to be capable of recognizing the presence, but not the identity, of urinary opioids, namely codeine (COD), codeine-6-glucuronide, dihydrocodeine (DHC), dihydrocodeine-6-glucuronide, morphine (MOR), morphine-3-glucuronide and ethylmorphine (EMOR). In these approaches, aliquots of urine and immunoreagents of a commercial, broadly cross-reacting fluorescence polarization immunoassay for opiates were combined and analyzed by capillary zone electrophoresis or micellar electrokinetic capillary chromatography with laser induced fluorescence detection. With the fluorescent tracer solution employed, the former method is shown to provide simple electropherograms which are characterized by an opioid concentration dependent magnitude of the free tracer peak. In presence of dodecyl sulfate micelles, however, two tracer peaks with equal opioid concentration sensitivity are monitored. These data suggest the presence of two fluorescent tracers which react competitively with the urinary opioids for the binding sites of the antibody. Assay sensitivities for COD and MOR are comparable (10 ng/ml), whereas those for DHC and EMOR are about four-fold lower. Furthermore, glucuronides are shown to react like the corresponding free opioids. Analysis of urines that were collected after administration of 7 mg COD and 25 mg DHC tested positively in both assay formats. The presence of the free and conjugated codeinoids in these urines and their identification was accomplished by capillary electrophoresis-ion trap mass spectrometry (CE-MS). This confirmatory assay is based upon solid-phase extraction using a mixed-mode polymer cartridge followed by CE hyphenated to the LCQ mass spectrometer with electrospray ionization in the positive ion mode. With this technology, MS2 is employed for proper identification of COD (m/z 300.4) and DHC (m/z 302.4) whereas MS3 provides unambiguous identification of the glucuronides of COD (m/z 476.5) and DHC (m/z 478.5) via their fragmentation to COD and DHC, respectively. MSn (n > or = 2) is shown to be capable of properly identifying the urinary codeinoids on the 100-200 ng/ml concentration level.     

Capillary electrophoresis in clinical and forensic analysis: recent advances and breakthrough to routine applications

This paper is a comprehensive review article on capillary electrophoresis (CE) in clinical and forensic analysis. It is based upon the literature of 1997 and 1998, presents CE examples in major fields of application, and provides an overview of the key achievements encountered, including those associated with the analysis of drugs, serum proteins, hemoglobin variants, and nucleic acids. For CE in clinical and forensic analysis, the past two years witnessed a breakthrough to routine applications. As most coauthors of this review are associated with diagnostic or forensic laboratories now using CE on a routine basis, this review also contains data from routine applications in drug, protein, and DNA analysis. With the first-hand experience of providing analytical service under stringent quality control conditions, aspects of quality assurance, assay specifications for clinical and forensic CE and the pros and cons of this maturing, cost-and pollution-controlled age technology are also discussed.     

Optical bit-pattern recognition by use of dynamic gratings in erbium-doped fiber

We present a novel optical bit-pattern-recognition technique that uses erbium-doped fiber at room temperature. Counterpropagating beams write a patterned gain-depletion grating in pumped erbium-doped fiber. This grating, recorded in the erbium gain medium, can be used for correlation with other optical bit patterns. We have demonstrated correlation of arbitrary return-to-zero bit patterns of as many as 8 bits at 1 Gbit/s . Theory suggests that scaling to higher bit rates is feasible.     

Sustainable use of eggshell powder in the composite based on recycled polystyrene and virgin polystyrene mixture

One of the ideas to recycle plastic waste is to mix it with virgin plastic in order to produce sustainable material. In this study, recycled polystyrene (R-PS) is used as a mixing component with virgin polystyrene (PS). Besides, eggshell powder (ESP) was also incorporated to reduce the compounding cost of the composite. Effects of R-PS/PS weight percentage on properties of composites were studied. Increasing PS content has increased tensile properties and impact strength of composites. Results obtained from these properties are in good relation to SEM micrographs. More surprisingly, R-PS plays a crucial role in enhancing the thermal stability of composites. This is simply due to the impurities contained in R-PS.     

Characterization of the Interactions of Lysozyme with DNA by Surface Plasmon Resonance and Circular Dichroism Spectroscopy

Association with nucleic acid has been recognized as a unique role of lysozyme and may explain why lysozyme was called a killer protein against HIV infection. In the present study, we characterized the interactions of lysozyme and its derived peptides with a biotin-labeled pUC19 plasmid DNA. Real-time detection of the macromolecular interaction was performed using the SPR (surface plasmon resonance) spectroscopy. The SPR sensorgrams were analyzed and the association and dissociation rate constants as well as the dissociation equilibrium constant K _D were, thus, estimated. The results reveal that other than the electrostatic interactions between the basic protein and the nucleotide sequences carrying negative charges, the specific DNA-binding motifs at the N- and C-termini of lysozyme were also involved in the interactions. The nonapeptide RAWVAWRNR (aa 107–115 of lysozyme) reported previously to block HIV-1 viral entrance and replication was also able to bind DNA with its K _D value comparable to that of histones. The possibilities of ligand-binding-induced conformational changes were investigated using the circular dichroism spectroscopy. The CD spectra (200–320 nm) reveal that the conformational changes indeed occur as the spectra of lysozyme–DNA interactions are much less at the major trough region than the sum of individual spectra. The interaction of lysozyme with DNA molecules may interfere with DNA replication, modulate gene expression, and block bacterial and viral infections. These all suggest that human lysozyme may represent part of the innate immune system with a very broad protective spectrum.     

Level properties of the light even Sb nuclei 112, 114, 116Sb

The level properties of ^{112, 114, 116}Sb have been investigated with the reactions ^112,114,116Sn(p, nγ)^112,114,116Sb and ¹¹⁵In(³He, xnγ)^112,114Sb (x = 6, 4). Singles γ and electron spectra, neutron-γ coincidences, γ-γ coincidences, excitation functions and half-lives were measured. Hauser-Feshbach calculations were performed for spin assignments. A number of new levels were found, including two 8^? isomeric states. The results are compared with recently performed 2qp calculations.     

Capillary electrophoresis-electrospray ionization ion trap mass spectrometry for analysis and confirmation testing of morphine and related compounds in urine

Using an aqueous background electrolyte containing 25 mM ammonium acetate and NH3 (pH 9), CE-tandem MS and CE-triple MS with atmospheric pressure electrospray ionization in the positive ion mode are shown to represent attractive approaches for analysis and confirmation testing of morphine (MOR) and related opioids in human urine. Injection of plain or diluted urine permits monitoring of solutes at concentrations above 2-5 microg/ml. For the recognition of lower concentrations, solute extraction and concentration is required. Liquid-liquid extraction at alkaline pH is shown to be suitable for analysis of free opioids only whereas solid-phase extraction using a mixed-mode polymer phase is demonstrated to permit analysis of both free and glucuronidated opioids. The former sample preparation approach, however, requires about half of the time only. Commencing with 2 ml of urine, reconstitution to provide a sample volume of 0.2 ml and hydrodynamic sample injection, detection limits for free opioids are shown to be on the 100-200 ng/ml drug level. Much improved (ppb) sensitivity is obtained by infusing the extract directly into the source of the MS system. However, solutes that produce equal fragments (such as the two glucuronides of MOR) can thereby not be distinguished. CE-tandem MS and CE-triple MS are demonstrated to be suitable to confirm the presence of MOR, MOR-3-glucuronide, 6-monoacetylmorphine, codeine, codeine-6-glucuronide, dihydrocodeine, methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in a toxicological quality control urine. The same is shown for selected metabolites of codeine and dihydrocodeine in urines collected after administration of pharmaceutical preparations.