Label-free cell separation and sorting in microfluidic systems

Cell separation and sorting are essential steps in cell biology research and in many diagnostic and therapeutic methods. Recently, there has been interest in methods which avoid the use of biochemical labels; numerous intrinsic biomarkers have been explored to identify cells including size, electrical polarizability, and hydrodynamic properties. This review highlights microfluidic techniques used for label-free discrimination and fractionation of cell populations. Microfluidic systems have been adopted to precisely handle single cells and interface with other tools for biochemical analysis. We analyzed many of these techniques, detailing their mode of separation, while concentrating on recent developments and evaluating their prospects for application. Furthermore, this was done from a perspective where inertial effects are considered important and general performance metrics were proposed which would ease comparison of reported technologies. Lastly, we assess the current state of these technologies and suggest directions which may make them more accessible.     

Theory of atomic diffraction from evanescent waves

We review recent theoretical models and experiments dealing with the diffraction of neutral atoms by a reflection grating, formed by a standing evanescent wave. We analyze diffraction mechanisms proposed for normal and grazing incidence, point out their scopes and confront the theory with experiment. Received: 12 June 1999 / Published online: 8 September 1999     

Modeling and experimental investigation of methylcyclohexane ignition in a rapid compression machine

A new chemical kinetic reaction mechanism has been developed for the oxidation of methylcyclohexane (MCH), combining a new low temperature mechanism with a recently developed high temperature mechanism. Predictions from this kinetic model are compared with new experimentally measured ignition delay times from a rapid compression machine. Computed results were found to be particularly sensitive to isomerization rates of methylcyclohexylperoxy radicals. Three different methods were used to estimate rate constants for these isomerization reactions. Rate constants based on comparable alkylperoxy radical isomerizations corrected for the differences in the structure of MCH and the respective alkane, predicted ignition delay times in very poor agreement with the experimental results. The most significant drawback was the complete absence of a region of negative temperature coefficient (NTC) in the model results using this method, although a prominent NTC region was observed experimentally. Alternative estimates of the isomerization reaction rate constants, based on the results from previous experimental studies of low temperature cyclohexane oxidation, provided much better agreement with the present experiments, including the pronounced NTC behavior. The most important feature of the resulting methylcyclohexylperoxy radical isomerization reaction analysis was found to be the relative rates of isomerizations that proceed through 5-, 6-, and 7-membered transition state ring structures and their different impacts on the chain branching behavior of the overall mechanism. Theoretical implications of these results are discussed, with particular attention paid to how intramolecular H atom transfer reactions are influenced by the differences between linear alkane and cycloalkane structures.     

Atom chips in the real world: the effects of wire corrugation

We present a detailed model describing the effects of wire corrugation on the trapping potential experienced by a cloud of atoms above a current carrying micro wire. We calculate the distortion of the current distribution due to corrugation and then derive the corresponding roughness in the magnetic field above the wire. Scaling laws are derived for the roughness as a function of height above a ribbon shaped wire. We also present experimental data on micro wire traps using cold atoms which complement some previously published measurements [CITE] and which demonstrate that wire corrugation can satisfactorily explain our observations of atom cloud fragmentation above electroplated gold wires. Finally, we present measurements of the corrugation of new wires fabricated by electron beam lithography and evaporation of gold. These wires appear to be substantially smoother than electroplated wires.     

Thermomechanical modification of diffraction gratings

The most accurate approaches to fabrication of diffraction gratings are known to be the lithographic and holographic methods. The lithographic methods allow fabrication of arbitrarily chirped gratings whose performance, however, is degraded by stitching errors. The holographic methods are free from stitching errors; however, they are limited in the achievable spatial variations of their grating periods. We suggest a method of diffraction grating modification by nonuniform heating and stretching that is much more flexible than the holographic approach and does not suffer from the problem of stitching error. We demonstrate our approach for quartz phase masks that have a characteristic grating period of 1 microm and a length of several centimeters. Our approach allows the grating periods of the phase masks to vary in a range from a few picometers to a few nanometers and a spatial resolution of a few millimeters. It is shown that the grating period can be modified with a negligible effect on the profile of the gratings.     

Influence of the longitudinal mode field in grating scattering from weakly guided optical fiber waveguides

We measured the polarization dependence of light scattered from a tilted fiber grating and found disagreement with previous volume-current perturbation analysis. However, by including the longitudinal E field of the guided wave we were able to obtain good agreement, demonstrating that, although it is small, this component cannot be neglected when scattering of weakly guided waves is considered. A first-order approximation formula for the polarization dependence was also obtained and is shown to be accurate within most of the resonance band of scattering.     

Two-photon fourth-order polarimetery

We demonstrate a fourth-order polarimeter that employs two-photon detection to measure the fourth-order polarization coherency matrix (FOCM) of an optical signal. We measure the FOCM of picosecond pulses subjected to polarization mode dispersion (PMD). The fourth-order polarimeter distinguishes different PMD states even when the state of polarization, including the degree of polarization, is constant.     

Getting the elastic scattering length by observing inelastic collisions in ultracold metastable helium atoms

We report an experiment measuring simultaneously the temperature and the flux of ions produced by a cloud of triplet metastable helium atoms at the Bose-Einstein critical temperature. The onset of condensation is revealed by a sharp increase of the ion flux during evaporative cooling. Combining our measurements with previous measurements of ionization in a pure Bose-Einstein condensate, we extract an improved value of the scattering length a=11.3(+2.5)(-1.0) nm. The analysis includes corrections that take into account the effect of atomic interactions on the critical temperature, and thus an independent measurement of the scattering length would allow a new test of these calculations.     

Ultraviolet photosensitive response in an antimony-doped optical fiber

A silica optical fiber doped with Sb is fabricated with a refractive-index profile that is comparable with standard single-mode fiber. In D(2)-loaded samples, we observe UV photosensitivity with an initial refractive-index-modulation growth rate six times higher than that of the equivalent Ge-doped standard fibers. Enhanced temperature stability of the Bragg grating strength up to 200 degrees C is also observed. Grating growth kinetics in the Sb-doped fiber is compared with those of other Ge-doped photosensitive fibers.     

Ionization rates in a bose-einstein condensate of metastable helium

We have studied ionizing collisions in a BEC of metastable He. Measurements of the ion production rate combined with measurements of the density and number of atoms for the same sample allow us to estimate both the two- and three-body contributions to this rate. A comparison with the decay of the atom number indicates that ionizing collisions are largely or wholly responsible for the loss. Quantum depletion makes a substantial correction to the three-body rate constant.