几种螯合剂解镍致小鼠胰脏毒作用比较

小鼠腹腔注射氯化镍溶液(5 mg Ni/kg)染毒，观察了N-苯甲基-D-葡糖氨基二硫代甲酸钠(BGD)、二乙氨基二硫代甲酸钠(DDTC)、二羟乙氨基二硫代甲酸钠(DHED)及meso-2，3-二巯基丁二酸钠(DMSA)等螯合剂对镍致小鼠胰脏毒性的解毒作用。镍染毒后引起小鼠胰脏胰蛋白酶活性升高和羧肽酶A活性降低以及组织中镍、钙、锌浓度增加；镍染毒30 min和24 h后进行各螯合剂治疗(剂量均为400 μmol/kg)，BGD、DDTC和DMSA可明显抑制上述变化，其中BGD解镍毒效果最好、自身毒性最小、对镍致小鼠胰脏毒性有更好的解毒作用。     

1,9—双（1′—苯基—3′—甲基—5′—氧代吡唑—4′—基）壬二酮—（1,9）与三？…

研究了１，９－双（１′－苯基－３′－甲基－５′－氧代吡唑－４′－基）壬二酮－（１，９）（ＢＰＭＰＮＤ，Ｈ２Ａ）与三辛基氧膦（ＴＯＰＯ，Ｂ）的氯仿溶液，从硝酸介质中对Ｌａ（Ⅲ），Ｄｙ（Ⅲ）的协同萃取，计算了体系的酸性协萃系数ＲＡ和协萃系数Ｒ，用斜率法测得协萃合物的组成ＬａＡ．ＨＡ．Ｂ和ＤｙＡ．ＨＡ．Ｂ计算了协萃取平衡常数，研究了温度对协萃反应的影响，用萃取法制得了固态协萃合物，并对其组成，ＩＲ及ＴＧ     

Theoretical and experimental studies of the electrochemistry of <Emphasis Type="Italic">p</Emphasis>-aminophenol on a golden electrode

The geometric parameters, vibrational frequencies, and thermochemical values of p-quinonimine (p-AQ) and p-aminophenol (p-AP) were computed ab initio (IIF) and by the density functional theory (DFT) method with the 6-31G(d, p) basis set. Cyclic voltammetry with a golden electrode of p-AP solutions in phosphate buffers at pH 7.30 showed that the standard electrode potential of half reaction for p-QI and p-AP was 0.728 V. The standard electrode potentials of half reactions for p-QI and p-AP were calculated using the free energies and solvation energies of p-QI, p-AP, p-benzoquinone (p-BQ), and hydroquinone (p-HQ). The results showed that the standard electrode potential of half reaction for p-QI and p-AP was 0.743 V at the B3LYP/6-31G(d, p) level and 0.755 V at the HF/6-31G(d, p) level. The standard electrode potentials computed at the B3LYP/6-31G(d, p) and HF/6-31G(d, p) levels were close to their experimental values. The article is published in the original.     

Plasmid DNA isolation using amino-silica coated magnetic nanoparticles (ASMNPs)

A simple and efficient approach for the rapid isolation of plasmid DNA from crude cell lysates has been described. The approach took advantage of the amino-modified silica coated magnetic nanoparticles (ASMNPs) with positive zeta potential at neutral pH and superparamagnetism under the external magnetic fields. As a demonstration, the pEGFP-N3 plasmid has been concentrated and isolated from the E. coli DH5α transformed with pEGFP-N3 plasmid through electrostatic binding between the positive charge of the amino group of ASMNPs and the negative charge of the phosphate groups of the plasmid DNA. Then the pEGFP-N3 plasmid has been released easily and quickly from the pEGFP-N3 plasmid-ASMNPs complexes with 3 M NaCl. The entire procedure could be carried out by the aid of external magnetic fields in 15 min and eliminate the need of phenol, cesium chloride gradients or other noxious reagents and complexes operation. Moreover, the pEGFP-N3 plasmid obtained by this approach retains biological activity that can be suitable for restriction enzyme digestion and cells transfection with expression of green fluorescence protein.     

High-pressure adsorption of CO2 on NaY zeolite and model prediction of adsorption isotherms

Supercritical carbon dioxide is an efficient solvent for adsorptive separations because it can potentially be used as both the carrier solvent for adsorption and the desorbent for regeneration. Recent results have demonstrated an anomalous peak or "hump" in the adsorption isotherm near the bulk critical point when the adsorption isotherm is plotted as a function of bulk density. This work presents new data for the adsorption and desorption of carbon dioxide in the near-critical region on a crystalline, well-structured adsorbent (NaY zeolite). The results indicate a strong affinity for CO(2) as well as a significant hump near the critical point. The lattice model previously developed by Aranovich and Donohue is applied to analyze the adsorption.     

Synthesis and crystal structure of a molybdenum complex of fullerene, [Mo(η 2–C60)(CO)2(dpphen)(dbf)·0.63CS2] (dpphen = 4,7-diphenyl-1,10-phenanthroline; dbf = dibutyl fumarate)

The molybdenum complex [Mo( ²-C₆₀)(CO)₂(dpphen)(dbf)] (1) [dpphen = 4,7-diphenyl-1,10-phenanthroline; dbf = dibutyl fumarate] has been prepared and structurally characterized by X-ray diffraction analysis. In the molecule of (1), the coordination geometry of the Mo atom is distorted octahedral with the phen (1,10-phenanthroline) group of dpphen and two CO groups in the equatorial plane. The Mo atom binds in an ²-fashion to the C—C bonds of C₆₀and dbf. The two C—C bonds are almost mutually orthogonal.     

Synergistic effects in mixed gas chromatographic stationary phases containing hepatkis(2.3.6-tri-O-pentyl)-β-cyclodextrin and AgNO3 or TINO3 in the separation of xylene isomers

Summary The gas chromatographic separation of xylene isomers using special mixed stationary phases consisting of heptakis(2.3.6-tri-O-pentyl)-β-CD-AgNO₃, heptakis (2.3.6-tri-O-pentyl)-β-CD-TINO₃ or bentone-34-AgNO₃ is investigated. The first two have positive synergistic effects for the separation of m, and p-xylene isomers, but negative synergistic effects for the m, and oisomers. The separation effects depend on temperature, mixing ratio and how mixing is accomplished.     

锌与小儿神经系统及代谢性疾病

综述了锌在小儿神经系统疾病和内分泌代谢性疾病中的作用及临床意义，尤其是对癫痫的发病起重要作用，大量资料证明高锌与癫痫发作有关，其血锌值明显高于健康儿童，因此在治疗中不要轻易补锌。还综述了锌与智能发育障碍、生长发育障碍、多动症、胎儿畸形、遗传性感音神经性聋等疾病的关系及在治疗糖尿病、肝豆状核变性等内分泌和代谢性疾病时要注意维持锌的正常动态平衡。     

A reliable ab initio potential energy surface and vibrational states for the ground electronic state of HgH2(X1Sigma+g)

A three-dimensional global potential energy surface for the ground (X (1)Sigma(+)(g))electronic state of HgH(2) is constructed from more than 13,00 ab initio points. These points are generated using an internally contracted multireference configuration interaction method with the Davidson correction and a large basis set. Low-lying vibrational energy levels of HgH(2), HHgD, and HgD(2) calculated using the Lanczos algorithm are found to be in good agreement with the available experimental band origins. The majority of the vibrational energy levels up to 9000 cm(-1) are assigned with normal mode quantum numbers. Our results indicate a gradual transition for the stretching vibrations from the normal mode regime at low energies to the local mode regime near 9000 and 8000 cm(-1) for HgH(2) and HgD(2), respectively, as evidenced by a decreasing energy gap between the (0,0,n(3)) and (1,0,n(3)-1) vibrational states and bifurcation of the corresponding wave functions.     

Direct electrochemical detection of glucose in human plasma on capillary electrophoresis microchips

We developed an electrochemical detector on a hybrid chip for the determination of glucose in human plasma. The microchip system described in this paper consists of a poly(dimethylsiloxane) (PDMS) layer containing separation and injection channels and an electrode plate. The copper microelectrode is fabricated by selective electroless deposition. The fabrication of the decoupler is performed by platinum electrochemical deposition on the metal film formed by electroless deposition. Factors influencing the performance, including detection potential, separation field strength, and buffer concentration, were studied. The electrodes exhibited good stability and durability in the analytical procedures. Under optimized detection conditions, glucose responded linearly from 10 microM to 1 mM. Finally, glucose in human plasma from three healthy individuals and two diabetics was successfully determined, giving a good prospect for a new clinical diagnostic instrument.