全文获取类型
收费全文 | 67篇 |
免费 | 0篇 |
专业分类
化学 | 34篇 |
力学 | 1篇 |
数学 | 3篇 |
物理学 | 29篇 |
出版年
2020年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2012年 | 2篇 |
2011年 | 5篇 |
2010年 | 2篇 |
2009年 | 2篇 |
2007年 | 1篇 |
2006年 | 1篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 5篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1977年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有67条查询结果,搜索用时 0 毫秒
31.
Dr. Mathieu Denis Dr. Jessica Pancholi Dr. Kajally Jobe Prof. Dr. Michael Watkinson Prof. Dr. Stephen M. Goldup 《Angewandte Chemie (International ed. in English)》2018,57(19):5310-5314
Although metal‐ion‐binding interlocked molecules have been under intense investigation for over three decades, their application as scaffolds for the development of sensors for metal ions remains underexplored. In this work, we demonstrate the potential of simple rotaxanes as metal‐ion‐responsive ligand scaffolds through the development of a proof‐of‐concept selective sensor for Zn2+. 相似文献
32.
The biotin-(strept)avidin interaction remains a gold standard of model biological recognition events. The biotinylation of azamacrocycles permits the investigation of signal transduction between this recognition event and the metal center of an azamacrocycle complex, of wide potential interest in biosensing. There are no generally applicable procedures in the literature for such functionalizations. We report here a comprehensive investigation into the attachment of biotin to TACN, cyclen, and cyclam. Effective methods have been found for each ring. The efficacy of the functionalization is critically dependent on the nature of the azamacrocycle. 相似文献
33.
Emiliano Tamanini Dr. Stephen E. J. Rigby Dr. Majid Motevalli Matthew H. Todd Dr. Michael Watkinson Dr. 《Chemistry (Weinheim an der Bergstrasse, Germany)》2009,15(15):3720-3728
Click to detect! Azamacrocyclic complexes with a triazole scorpion ligand may be easily assembled, as shown for a biotin‐functionalised cyclam derivative. Coordination of the triazole to the metal is perturbed by the binding of avidin to the pendant ligand (see scheme). This event can be sensitively detected with EPR and ENDOR spectroscopy, which confirm the loss of the axial triazole nitrogen donor upon avidin binding. This general strategy may have wide applications in imaging and therapeutics.
34.
35.
36.
37.
38.
39.
40.
Harris JR Soliakov A Lewis RJ Depoix F Watkinson A Lakey JH 《Micron (Oxford, England : 1993)》2012,43(2-3):192-200
Aluminium-based vaccine adjuvants have been in use since the 1920s. Aluminium hydroxide (alum) that is the chemical basis of Alhydrogel, a widely used adjuvant, is a colloid that binds proteins to the particular surface for efficient presentation to the immune system during the vaccination process. Using conventional TEM and cryo-TEM we have shown that Alhydrogel can be finely dispersed by ultrasonication of the aqueous suspension. Clusters of ultrasonicated aluminium hydroxide micro-fibre crystals have been produced (~ 10-100 nm), that are significantly smaller than those present the untreated Alhydrogel (~ 2-12 μm). However, even prolonged ultrasonication did not produce a homogenous suspension of single aluminium hydroxide micro-fibres. The TEM images of unstained and negatively stained air-dried Alhydrogel are very similar to those obtained by cryo-electron microscopy. Visualization of protein on the surface of the finely dispersed Alhydrogel by TEM is facilitated by prior ultrasonication. Several examples are given, including some of medical relevance, using proteins of widely ranging molecular mass and oligomerization state. Even with the smaller mass proteins, their presence on the Alhydrogel surface can be readily defined by TEM. It has been found that low quantities of protein tend to cross-link and aggregate the small Alhydogel clusters, in a more pronounced manner than high protein concentrations. This indicates that complete saturation of the available Alhydrogel surface with protein may be achieved, with minimal cross-linkage, and future exploitation of this treatment of Alhydrogel is likely to be of immediate value for more efficient vaccine production. 相似文献