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A system is described for the on-line extraction of a digested protein in a gel spot. The extract obtained was on-line transferred to a precolumn, used to desalinate and preconcentrate the sample. The precolumn was switched in-line with an LC-ESI-MS system to separate the digest products prior to detection. By using this approach sample losses were minimized and the sample was quantitatively transferred. The benefits of the system were shown for the in-gel tryptic digest of several model proteins. Using this system, the sensitivity was 13-fold increased compared to the off-line extraction of in-gel digest products leading to a higher sequence coverage and thus a better identification potential. Also, the within day repeatability was a factor 2.5 improvement using the on-line procedure compared to the off-line extraction procedure. 相似文献
24.
L A van Ginkel E H Jansen R W Stephany P W Zoontjes P L Schwillens H J van Rossum T Visser 《Journal of chromatography. A》1992,624(1-2):389-401
The role of liquid chromatography within methods of analysis for steroids, related compounds and beta-agonists in biological samples is discussed. Special attention is given to the application of liquid chromatography in sample preparation and extract clean-up. Different forms of liquid chromatography, including immunoaffinity chromatography, are compared and evaluated. Methods for confirmation based on gas chromatography-mass spectrometry and cryotrapping Fourier transform infrared spectrometry are discussed. 相似文献
25.
A method is given for determining the general formula UO(2+x), of sintered uranium dioxide pellets and uranium dioxide powders. Uranium(VI) is reduced by titration with iron(II) ammonium sulphate, after which the total amount of uranium is oxidized by titration with potassium dichromate. The end-points of both titrations are detected electrometrically. Determination of x in the range 0.0001-1.00 is possible. 相似文献
26.
Rob Visser 《Accreditation and quality assurance》2004,9(11-12):717-723
Following the introduction of ISO 17025 in 1999, all accredited testing laboratories have started to implement a procedure for estimating measurement uncertainties for the analytical methods that they employ. These procedures and uncertainties are an integral part of the quality system of the accredited laboratory and are therefore reviewed during audits by the accreditation bodies. In January/February 2004 the status of measurement uncertainties available at accredited testing laboratories was evaluated. It was found that most laboratory customers do not make use nor want to make use of the available measurement uncertainties. Furthermore, the current lack of standardisation has caused the estimated measurement uncertainties to vary widely, even for the simplest determinations. 相似文献
27.
Colangelo J Licon V Benen J Visser J Bergmann C Orlando R 《Rapid communications in mass spectrometry : RCM》1999,13(23):2382-2387
Recombinant pectate lyase from Aspergillus niger was overexpressed in Aspergillus nidulans. The two recombinant proteins produced differed in molecular mass by 1200 Da, which suggested that the larger molecular weight protein was glycosylated. The deduced amino acid sequence was searched for potential N-linked glycosylation sites, and one potential site was identified at residue 64. The proteins were analyzed for their ability to bind various lectins as an assay for the presence of carbohydrates. The proteins were then digested with trypsin to facilitate the isolation of the potential glycosylation site. The resulting digestion products were subsequently analyzed by liquid chromatography/mass spectrometry using in-source collision induced dissociation to detect glycopeptides. Once the glycopeptide had been identified, treatment with an endoglycosidase both verified the location of glycosylation and identified the mass of the glycan. The Complex Carbohydrate Structural Database was searched for possible N-linked structures with the same mass, and the suggested primary sequence was confirmed by an exoglycosidase digestion. The data demonstrated that the larger recombinant protein contained a high mannose N-linked structure (Man(5)GlcNAc(2)) attached to N-64, while this site was not occupied in the smaller protein. 相似文献
28.
Soga O van Nostrum CF Ramzi A Visser T Soulimani F Frederik PM Bomans PH Hennink WE 《Langmuir : the ACS journal of surfaces and colloids》2004,20(21):9388-9395
Amphiphilic AB block copolymers consisting of thermosensitive poly(N-(2-hydroxypropyl) methacrylamide lactate) and poly(ethylene glycol), pHPMAmDL-b-PEG, were synthesized via a macroinitiator route. Dynamic light scattering measurements showed that these block copolymers form polymeric micelles in water with a size of around 50 nm by heating of an aqueous polymer solution from below to above the critical micelle temperature (cmt). The critical micelle concentration as well as the cmt decreased with increasing pHPMAmDL block lengths, which can be attributed to the greater hydrophobicity of the thermosensitive block with increasing molecular weight. Cryogenic transmission electron microscopy analysis revealed that the micelles have a spherical shape with a narrow size distribution. 1H NMR measurements in D2O showed that the intensity of the peaks of the protons from the pHPMAmDL block significantly decreased above the cmt, indicating that the thermosensitive blocks indeed form the solidlike core of the micelles. Static light scattering measurements demonstrated that pHPMAmDL-b-PEG micelles with relatively large pHPMAmDL blocks possess a highly packed core that is stabilized by a dense layer of swollen PEG chains. FT-IR analysis indicated that dehydration of amide bonds in the pHPMAmDL block occurs when the polymer dissolved in water is heated from below to above its cmt. The micelles were stable when an aqueous solution of micelles was incubated at 37 degrees C and at pH 5.0, where the hydrolysis rate of lactate side groups is minimized. On the other hand, at pH 9.0, where hydrolysis of the lactic acid side groups occurs, the micelles started to swell after 1.5 h of incubation and complete dissolution of micelles was observed after 4 h as a result of hydrophilization of the thermosensitive block. Fluorescence spectroscopy measurements with pyrene loaded in the hydrophobic core of the micelles showed that when these micelles were incubated at pH 8.6 and at 37 degrees C the microenvironment of pyrene became increasingly hydrated in time during this swelling phase. The results demonstrate the potential applicability of pHPMAmDL-b-PEG block copolymer micelles for the controlled delivery of hydrophobic drugs. 相似文献
29.
de Visser SP Shaik S Sharma PK Kumar D Thiel W 《Journal of the American Chemical Society》2003,125(51):15779-15788
The active site of HRP Compound I (Cpd I) is modeled using hybrid density functional theory (UB3LYP). The effects of neighboring amino acids and of environmental polarity are included. The low-lying states have porphyrin radical cationic species (Por(*)(+)). However, since the Por(*)(+) species is a very good electron acceptor, other species, which can be either the ligand or side chain amino acid residues, may participate in electron donation to the Por(*)(+) moiety, thereby making Cpd I behave like a chemical chameleon. Thus, this behavior that was noted before for Cpd I of P450 is apparently much more wide ranging than initially appreciated. Since chemical chameleonic behavior property was found to be expressed not only in the properties of Cpd I itself, but also in its reactivity, the roots of this phenomenon are generalized. A comparative discussion of Cpd I species follows for the enzymes HRP, CcP, APX, CAT (catalase), and P450. 相似文献
30.
KINETICS OF STACKING INTERACTIONS IN FLAVIN ADENINE DINUCLEOTIDE FROM TIME-RESOLVED FLAVIN FLUORESCENCE 总被引:3,自引:0,他引:3
A. J. W. G. Visser 《Photochemistry and photobiology》1984,40(6):703-706
The time dependence of the fluorescence of flavin adenine dinucleotide (FAD) was measured with a subnanosecond-resolving fluorometer. In contrast to the fluorescence decay of FMN, the decay of FAD was proved to be nonexponential. The time-dependent fluorescence of FAD can be interpreted by assuming an equilibrium between closed and open conformers in the ground state. The rate constant for folding in the excited state and the fluorescence lifetime of the intramolecular complex could be evaluated from analysis of the observed fluorescence decay. The results on FAD were compared to those on NADH obtained earlier. 相似文献