Tear proteomics, by 2-DE, can give a fingerprint of the protein profile, which is well suited in clinical proteomics for biomarker identification and in diagnostics. The mode of tear collection can influence the representation of the proteins in the tear and therefore it is important to use the appropriate method. In this study, capillary and Schirmer mode of tear collection was done in the healthy controls and the Schirmer method was validated in dry eye syndrome conditions. 2-D PAGE of normal and dry eye tear was performed using pH 3-10 linear IPG strips followed by 13% SDS-PAGE. The spot intensity was analyzed by the PD quest software. The two methods were compared using Bland-Altman statistical tool. The 2-D map of capillary and Schirmer tear showed 147 ± 8 spots and 145 ± 7 spots respectively. Both the collection methods were in agreement with each other and were comparable. Dry eye tear protein showed differential expression of proteins as observed in 25-35 kDa region. One of the significantly reduced protein was identified as proline-rich 4 protein. Schirmer method of tear collection is reliable in patients with dry eye, which can display the differential protein expression and help in biomarker identification. 相似文献
Summary The i.r. spectral data on O-methyl-l-amidinourea hydrochloride,O-ethyl-1-amid inourea hydrochloride and the metal complexes [M = copper(H), nickel(II), palladium(II), zinc(II) and oxovanadium(IV)] ofO-methyl-l-amid inourea andO-ethyl-l-amidinourea are reported. The spectral results suggest that secondary amine nitrogens are the donor atoms inO-alkyl-1-amid inourea. The free ligands and the complexes exhibit a characteristic band at ca. 1100 cm–1 due to the C-OR group. The i.r. and electronic spectral data on the hydrated and anhydrous uncharged metal complexes indicate that the hydrated uncharged metal complexes ofO-alkyl-l-amidinourea may be represented as [M(AAU)n]] · xH20 and not as [M(AAUII)n](OH)n · xH2O [where AAU = H2NC(=NH)NC(=NH)OR and AAUH = H2NC(=NH)NHC(=NH)OR, R = Me, Et]. The use of the term anhydrobase is confusing and suitable names for various metal complexes ofO-alkyl-l-amid inourea are suggested. The main decomposition stage in metal complexes ofO-ethyl-l-amidinourea occurs at lower temperatures than for the corresponding biguanide complexes indicating that the former are less stable than the latter. 相似文献
The dispersal of CuO catalyst on the surface of the semiconducting SnO2 film is found to be of vital importance for improving the sensitivity and the response speed of a SnO2 gas sensor for H2S gas detection. Ultra-thin CuO islands (8 nm thin and 0.6 mm diameter) prepared by evaporating Cu through a mesh and subsequent
oxidation yield a fast response speed and recovery. Ultimately nanoparticles of Cu (average size = 15 nm) prepared by a chemical
technique using a reverse micelle method involving the reduction of Cu(NO3)2 by NaBH4 exhibited significant improvement in the gas sensing characteristics of SnO2 films. A fast response speed of ∼14 s and a recovery time of ∼60 s for trace level ∼20 ppm H2S gas detection have been recorded. The sensor operating temperature (130° C) is low and the sensitivity (S = 2.06 × 103) is high. It is found that the spreading over of CuO catalyst in the nanoscale range on the surface of SnO2 allows effective removal of excess adsorbed oxygen from the uncovered SnO2 surface due to spill over of hydrogen dissociated from the H2S-CuO interaction. 相似文献
Zinc oxide (ZnO) nanowhiskers have been prepared using a multilayer ZnO(50 nm)/Zn(20 nm)/ZnO(2μm) structure on a polished stainless steel (SS) substrate by high rate magnetron sputtering. The formation of uniformly distributed
ZnO nanowhiskers with about 20 nm dia. and 2 to 5 μm length was observed after a postdeposition annealing of the prepared
structure at 300–400° C. An array of highlyc-axis oriented ZnO columns (70–300 nm in dia. and up to 10 μm long) were grown on Si substrates by pulsed laser deposition
(PLD) at a high pressure (1 Torr), and Raman studies showed the activation of surface phonon modes. The nanosized powder (15–20
nm) and nanoparticle ZnO films on glass substrate were also prepared by a chemical route. Nanowhiskers showed enhanced UV
light detection characteristics, and the chemically prepared ZnO nanoparticle films exhibited good sensing properties for
alcohol 相似文献
It is established that increasing the amplitude of a flutter stimulus increases its perceived intensity. Although many studies
have examined this phenomenon with regard to the responding afferent population, the way in which the intensity of a stimulus
is coded in primary somatosensory cortex (SI) remains unclear. 相似文献
The sodium salts of the tris(2-mercapto-1-benzylimidazolyl)borate [TmBz]− and tris(2-mercapto-1-p-tolylimidazolyl)borate [Tmp-Tol]− anions have been readily prepared in very good yield from NaBH4 and 2-mercapto-1-benzylimidazole or 2-mercapto-1-p-tolylimidazole, respectively. These new monoanionic tripodal sulfur-donor ligands have been used to prepare the Group 12 derivatives (TmR)MBr (M=Zn, Cd; R=Bz, p-Tol), all of which have been characterized by a combination of analytical and spectroscopic techniques, including electrospray ionization mass spectrometry (ESI MS) and, in the case of both benzyl-substituted derivatives, single-crystal X-ray diffraction. 相似文献
An algorithm is developed for simulating adsorption of tree type block‐branched copolymer chains, of arbitrary architecture, from dilute solutions to solid surfaces. A continuum form of the self‐consistent field (SCF) theory is used. The chain architecture is first represented by a convergent tree‐graph, which is then converted into a special type of the connectivity matrix. This matrix is used for computing the configurational statistics of the chains in the adsorbed layer. The crucial step in the algorithm is to compute the junction (branch point) probability weights. A stepwise procedure for computing these probability weights is described. The capability of the algorithm has been demonstrated using illustrative examples.
Metal-glass-metal (MGM) thin film devices are prepared using vacuum deposition of Sb2Pb1Se7 compound. The variation of ac conductance as a function of thickness of films, temperature and frequency is studied. The
observed characteristics are explained on the basis of Co-related Barrier Hopping (CBH) of bipolaron. It is shown that the
theoretical curve generated using the first order approximation gives excellent fitting with the experimental curve. 相似文献