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排序方式: 共有520条查询结果,搜索用时 31 毫秒
451.
Verdiana Covelli Manuela Grimaldi Rosario Randino Mohammad Firoznezhad Maria Chiara Proto Veronica De Simone Gianluca Matteoli Patrizia Gazzerro Maurizio Bifulco Anna Maria DUrsi Manuela Rodriquez 《Molecules (Basel, Switzerland)》2021,26(23)
N6-Isopentenyladenosine (i6A) is a naturally occurring modified nucleoside displaying in vitro and in vivo antiproliferative and pro-apoptotic properties. In our previous studies, including an in silico inverse virtual screening, NMR experiments and in vitro enzymatic assays, we demonstrated that i6A targeted farnesyl pyrophosphate synthase (FPPS), a key enzyme involved in the mevalonate (MVA) pathway and prenylation of downstream proteins, which are aberrant in several cancers. Following our interest in the anticancer effects of FPPS inhibition, we developed a panel of i6A derivatives bearing bulky aromatic moieties in the N6 position of adenosine. With the aim of clarifying molecular action of N6-benzyladenosine analogs on the FPPS enzyme inhibition and cellular toxicity and proliferation, herein we report the evaluation of the N6-benzyladenosine derivatives’ (compounds 2a–m) effects on cell viability and proliferation on HCT116, DLD-1 (human) and MC38 (murine) colorectal cancer cells (CRC). We found that compounds 2, 2a and 2c showed a persistent antiproliferative effect on human CRC lines and compound 2f exerted a significant effect in impairing the prenylation of RAS and Rap-1A proteins, confirming that the antitumor activity of 2f was related to the ability to inhibit FPPS activity. 相似文献
452.
Lorella Paparo Maria Antonia Maglio Maddalena Cortese Cristina Bruno Mario Capasso Erika Punzo Veronica Ferrucci Vito Alessandro Lasorsa Maurizio Viscardi Giovanna Fusco Pellegrino Cerino Alessia Romano Riccardo Troncone Massimo Zollo 《Molecules (Basel, Switzerland)》2022,27(3)
Butyrate is a major gut microbiome metabolite that regulates several defense mechanisms against infectious diseases. Alterations in the gut microbiome, leading to reduced butyrate production, have been reported in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. A new butyrate releaser, useful for all the known applications of butyrate, presenting physiochemical characteristics suitable for easy oral administration, (N-(1-carbamoyl-2-phenyl-ethyl) butyramide (FBA), has been recently developed. We investigated the protective action of FBA against SARS-CoV-2 infection in the human small intestine and enterocytes. Relevant aspects of SARS-CoV-2 infection were assessed: infectivity, host functional receptor angiotensin-converting enzyme-2 (ACE2), transmembrane protease serine 2 (TMPRSS2), neuropilin-1 (NRP1), pro-inflammatory cytokines expression, genes involved in the antiviral response and the activation of Nf-kB nuclear factor (erythroid-derived 2-like) 2 (Nfr2) pathways. We found that FBA positively modulates the crucial aspects of the infection in small intestinal biopsies and human enterocytes, reducing the expression of ACE2, TMPRSS2 and NRP1, pro-inflammatory cytokines interleukin (IL)-15, monocyte chemoattractant protein-1 (MCP-1) and TNF-α, and regulating several genes involved in antiviral pathways. FBA was also able to reduce the number of SARS-CoV-2-infected cells, and ACE2, TMPRSS2 and NRP1 expression. Lastly, through the inhibition of Nf-kB and the up-regulation of Nfr2, it was also able to reduce the expression of pro-inflammatory cytokines IL-15, MCP-1 and TNF-α in human enterocytes. The new butyrate releaser, FBA, exerts a preventive action against SARS-CoV-2 infection. It could be considered as an innovative strategy to limit COVID-19. 相似文献
453.
Elisabeth Holder Veronica Marin Michael A. R. Meier Ulrich S. Schubert 《Macromolecular rapid communications》2004,25(16):1491-1496
Summary : A monoterpyridine‐poly(ethylene glycol) (mono‐tpy‐PEG) and a novel monoterpyridine‐PEG‐functionalized iridium(III ) complex were successfully synthesized and fully characterized by means of NMR, IR, and UV‐vis spectroscopy, as well as gel permeation chromatography and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. The functionalized monoterpyridine iridium(III ) complex was synthesized by a bridge‐splitting reaction of a dimeric iridium(III ) precursor complex using a chelating terpyridine ligand with a poly(ethylene glycol) tail. With this approach, a new class of light‐emitting polymeric materials revealing interesting optical properties was made avaialable.
454.
Veronica Marin Elisabeth Holder Michael A. R. Meier Richard Hoogenboom Ulrich S. Schubert 《Macromolecular rapid communications》2004,25(7):793-798
Summary: An amino‐functionalized bipyridine ligand was prepared in order to serve as a bridging unit to an activated low‐molecular‐weight monomethyl ether of poly(ethylene glycol) (PEG). Coordination of a ruthenium(II ) phenantroline precursor onto the formed PEG‐containing bipyridine ligand yielded a metal‐containing polymer which shows interesting properties for solar cell applications.
455.
Lattanzio VM Gatta SD Suman M Visconti A 《Rapid communications in mass spectrometry : RCM》2011,25(13):1869-1880
A sensitive and robust liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the simultaneous determination of aflatoxins (B(1), B(2), G(1), G(2)), ochratoxin A, deoxynivalenol, zearalenone, T-2 and HT-2 toxins in cereal-based foods. Samples were extracted with a mixture of acetonitrile/water (84:16, v/v) and cleaned up through a polymeric solid-phase extraction column. Detection and quantification of the nine mycotoxins were performed by reversed-phase liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (LC/ESI-MS/MS), using fully (13)C-isotope-labelled mycotoxins as internal standards. The method was validated in-house for five different cereal processed products, namely barley, oat and durum wheat flours, rye- and wheat-based crisp bread. Recoveries and repeatability of the whole analytical procedure were evaluated at contamination levels encompassing the EU maximum permitted levels for each tested mycotoxin. Recoveries ranged from 89 to 108% for deoxynivalenol, from 73 to 114% for aflatoxins, from 85 to 114% for T-2 and HT-2 toxins, from 64 to 97% for zearalenone, from 74 to 102% for ochratoxin A. Relative standard deviations were less than 16% for all tested mycotoxins and matrices. Limits of detection (signal-to-noise ratio 3:1) ranged from 0.1 to 59.2 μg/kg. The trueness of the results obtained by the proposed method was demonstrated by analysis of reference materials for aflatoxins, deoxynivalenol, zearalenone. The use of inexpensive clean-up cartridges and the increasing availability of less expensive LC/MS/MS instrumentation strengthen the potential of the proposed method for its effective application for reliable routine analysis to assess compliance of tested cereal products with current regulation. 相似文献
456.
Bogialli S Di Corcia A Laganà A Mastrantoni V Sergi M 《Rapid communications in mass spectrometry : RCM》2007,21(2):237-246
A rapid and simple sample preparation procedure for determining residues of antibiotics of the class of macrolides and lincomycin in whole milk and yoghurt by liquid chromatography/tandem mass spectrometry (LC/MS/MS) is presented. The method is based on the matrix solid-phase dispersion (MSPD) technique with hot water as extractant. After dispersing samples of milk and yoghurt on sand, target compounds were eluted from the MSPD column by passing through it 5 mL of water acidified with 30 mmol/L formic acid and heated at 70 degrees C. After pH adjustment and filtration, a volume of 200 microL of the aqueous extract was directly injected into the LC column. MS data acquisition was generally performed in the multiple reaction monitoring (MRM) mode, selecting two precursor ion to product ion transitions for each target compound. Hot water appeared to be an efficient extracting medium, since absolute recoveries of the analytes in milk and yoghurt were respectively 68-86% and 82-96%. The method proved to be robust as matrix effects, even though present, did not affect significantly the accuracy of the method, as evidenced by analyzing six different batches of both milk and yoghurt. Using roxithromycin (a macrolide antibiotic not used in veterinary medicine) as surrogate internal standard, the accuracy of the method at three different spike levels of the analytes in milk and yoghurt was 86-107% (RSDs not larger than 10%) and 97-117% (RSDs not larger than 13%), respectively. On the basis of a signal-to-noise ratio of 10, we estimated this method can quantify a few ppb of the analytes in milk and yoghurt. These concentrations are well below the tolerance levels of macrolides and lincomycin in milk set by both the European Union and the US Food and Drug Administration. On analyzing six yoghurt samples, we found evidence for the fact that one of the six samples was contaminated with erythromycin B. 相似文献
457.
When diazepam in chloroform was incorporated into a KBr pellet, its infrared (i.r.) spectrum showed an anomalous spectral phenomenon at 836 cm?1. Examination of i.r. spectra of diazepam obtained by the solution, multiple-internal-reflectance, and KBr-pellet techniques suggest that this spectral characteristic is due to the orientation effect of diazepam in KBr pellets. 相似文献
458.
Veronica P. Martino Roxana A. Ruseckaite A. Jiménez 《Journal of Thermal Analysis and Calorimetry》2006,86(3):707-712
In this work amorphous poly(L-lactide-co-D,L-lactide) (PLLA/PDLLA) was blended with four different
commercial adipates to obtain films with enhanced mechanical and thermal properties.
Efficiency of plasticizers was evaluated by studying their compatibility with
the polymer and their effect on its glass transition temperature. All plasticizers
were compatible with the matrix up to a critical composition depending on
its molar mass. The addition of plasticizers caused a decrease in elastic
modulus and tensile stress, meanwhile elongation at break had a maximum increase
for polyadipates with the lower molar mass. Monomeric adipate showed some
migration at concentration higher than 10 mass%, while the addition of the
higher molar mass plasticizer lead to eventual phase separation. Polyadipates
with low molar mass showed a promising behaviour to overcome the brittleness
in PLLA/PDLLA films. 相似文献
459.
The screening of PAHs from seawater samples using cloud-point extraction (CPE) as a step prior to their determination by fluorescence measurements with a fiber-optic is proposed. The CPE is carried out with the nonionic surfactants mixture POLE and Brij 30. The fluorescence measurement parameters were optimized, allowing selection of benzo(a)pyrene (B(a)Py) and benzo(k)fluoranthene (B(k)Ft) as the target analytes for the screening. The reproducibility of the whole screening system, expressed as relative standard deviation, was 9.0% for B(a)Py and 12.1% for B(k)Ft (both for n = 7). The reliability of the method was established at five concentrations for B(a)Py (between 0.5 and 3.3 times the detection limit: 0.31 ng ml(-1)) and at three concentrations for B(k)Ft (between 0.6 and 2.5 times the detection limit: 0.56 ng ml(-1)). The resolution of binary mixtures of these PAHs at different levels of concentration, and a study of the interferences with the rest of the PAHs were also carried out. 相似文献
460.
1H NMR relaxation rates provide a readily available and sensitive probe ideally suited to investigate the weak (KD approximately micromolar to millimolar range) interactions that frequently mediate polypeptide oligomerization in the early steps of amyloid fibrillogenesis. However, the measurement of transverse and longitudinal 1H relaxation rates is experimentally challenging due to J-transfer and selectivity problems in CPMG and inversion-recovery experiments, respectively. We show here that these problems are effectively circumvented by measuring nonselective off-resonance relaxation rates using an effective field tilted by 35.5 degrees . When applied to the Halpha spins of the Abeta (12-28) peptide, the proposed experiment provides a residue-resolution self-recognition map which is fully consistent with previous independent mutational studies. The method is anticipated to be widely applicable not only to the fast growing family of amyloidogenic peptides but also to the screening and mapping of protein-ligand interactions in general. 相似文献