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81.
Synthesis of 1-oxa-3,8-diazospiro [4,5] decan-2-ones from 4-pyridone by addition of trimethylsilyl cyanide. 相似文献
82.
Juan Pablo Rendn Ana Isabel Caas Elizabeth Correa Vanesa Bedoya-Betancur Marlon Osorio Cristina Castro Tonny W. Naranjo 《Molecules (Basel, Switzerland)》2022,27(20)
Colorectal Cancer (CRC) ranks third in terms of incidence and second in terms of mortality and prevalence worldwide. In relation to chemotherapy treatment, the most used drug is 5-fluorouracil (5-FU); however, the use of this drug generates various toxic effects at the systemic level. For this reason, new therapeutic strategies are currently being sought that can be used as neoadjuvant or adjuvant treatments. Recent research has shown that natural compounds, such as genistein, have chemotherapeutic and anticancer effects, but the mechanisms of action of genistein and its molecular targets in human colon cells have not been fully elucidated. The results reported in relation to non-malignant cell lines are also unclear, which does not allow evidence of the selectivity that this compound may have. Therefore, in this work, genistein was evaluated in vitro in both cancer cell lines SW480 and SW620 and in the non-malignant cell line HaCaT. The results obtained show that genistein has selectivity for the SW480 and SW620 cell lines. In addition, it inhibits cell viability and has an antiproliferative effect in a dose-dependent manner. Increased production of reactive oxygen species (ROS) was also found, suggesting an association with the cell death process through various mechanisms. Finally, the encapsulation strategy that was proposed made it possible to demonstrate that bacterial nanocellulose (BNC) is capable of protecting genistein from the acidic conditions of gastric fluid and also allows the release of the compound in the colonic fluid. This would allow genistein to act locally in the mucosa of the colon where the first stages of CRC occur. 相似文献
83.
高温高密核物质相结构是核物理研究领域的热点和前沿。量子色动力学(QCD)相变临界点的实验确认将是探索核物质相结构的里程碑,具有重要科学意义。为了在这一具有潜在重大发现的研究方向上占据领先地位、取得突破,各国纷纷建造大型加速器以及粒子探测器,开展重离子碰撞实验,其主要目标就是从实验上探索高温高密核物质相结构、寻找QCD相变临界点。文章总结了近年来相对论重离子碰撞中核物质相结构及QCD临界点的实验研究进展,并对未来探索高重子密度区核物质相结构、寻找QCD临界点的重要实验装置做了展望。 相似文献
84.
Carmen Rodríguez Robelo Vanesa Zazueta Novoa Roberto Zazueta-Sandoval 《Applied biochemistry and biotechnology》2004,113(1-3):161-171
Soluble alcohol oxidase (AO) activity was detected in the supernatant fraction of a high-speed centrifugation procedure after
ballistic cellular homogenization to break the mycelium from a filamentous fungus strain named YR-1, isolated from petroleum-contaminated
soils. AO activity from aerobically grown mycelium was detected in growth media containing different carbon sources, including
alcohols and hydrocarbons but not in glucose. In previous work, zymogram analysis conducted with crude extracts from aerobic
mycelium of YR-1 strain indicated the existence of two AO enzymes originally named AO-1 and AO-2. In the present study, we
were able to separate the AO-1 band into two bands depending on culture conditions, carbon source, and polyacrylamide gel
electrophoresis (PAGE) separation conditions; the enzyme activity pattern in zymograms from cell-free extracts exhibited three
different bands after native PAGE. New nomenclature was used for upper bands AO-1 and AO-2 and lower band AO-3, respectively.
The expression of AO activity was studied in the absence of glucose in the culture media and in the presence of hydrocarbons
or petroleum as sole carbon source, suggesting that AO expression could be subjected to two regulatory possibilities: carbon
catabolite regulation by glucose and induction by hydrocarbons. The possibility of catabolic inhibition of AO by glucose in
the active enzyme was also tested, and the results confirm that this kind of regulatory mechanism is not present in AO activity. 相似文献
85.
A seminumeric method by Herrera, Jiménez and Ruggeri is used to study the evolution of general relativistic spheres in the free streaming radiation approximation, when specific contraction schemes are imposed and for particular oscillatory radiation profiles. On one hand, the emerging radiation is studied for oscillating contracting spheres, on the other, the variation of the boundary surface is analyzed when a pulsating profile is provided. We consider three particular equations of state previously studied. It is found that the oscillatory frequency of the surface for the equations of state considered coincides with the frequency of the oscillation for the radiation profile. 相似文献
86.
87.
The hydrolysis of flucloxacillin at pH 4.9 yields a degradation product which is polarographically oxidizable. This derivative has not been identified, but would seem to contain a thiol group. It gives a diffusion-controlled anodic polarographic wave with a half-wave potential at -0.24 V vs. SCE. The method developed has been applied to the analysis of flucloxacillin capsules, and a recovery of 99% has been obtained. 相似文献
88.
Alvarado-Caudillo Yolanda Torres José Carlos Bravo Novoa Vanesa Zazueta Jiménez Hortencia Silva Torres-Guzmán J. Carlos Gutiérrez-Corona J. Félix Zazueta-Sandoval Roberto 《Applied biochemistry and biotechnology》2002,98(1-9):243-255
A soluble alcohol oxidase (AO) activity was detected in the mycelium of a filamentous fungus strain named YR-1, isolated from
petroleum-contaminated soils. AO activity from aerobically grown mycelium was detected in growth media containing the hydrocarbons
decane or hexadecane; the enzyme activity exhibited optimum pH for the oxidation of different alcohols (methanol, ethanol,
and hexadecanol) similar to that of the corresponding aldehyde. Zymogram analysis conducted with purified fractions from aerobic
mycelium of YR-1 strain extracts indicated the existence of two AO enzymes (AO-1 and AO-2). Purified samples of both enzymes
analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis indicated the presence of three protein bands with molecular
sizes 20, 38, and 46 kDa that could be part of the native enzyme. In samples of both enzymes, the 46-kDa protein gave a positive
reaction in immunodetection experiments with antibodies directed against AO from Hansenula polymorpha. The purified AO-2 enzyme oxidized different alcohols, although higher activity was displayed with hexadecanol. K
m
values obtained for methanol and hexa-decanol indicated a higher affinity for the latter. Analysis of the aminoter-minal
sequence of the 46-kDa protein of AO-2 enzyme indicated significant similarity to enzymes involved in the metabolism of biphenyl
polychloride compounds. 相似文献
89.
Simultaneous monitoring of monoamines,amino acids,nucleotides and neuropeptides by liquid chromatography‐tandem mass spectrometry and its application to neurosecretion in bovine chromaffin cells
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Aneta Wojnicz José Avendaño‐Ortiz Ricardo de Pascual Lucía Ruiz‐Pascual Antonio G. García Ana Ruiz‐Nuño 《Journal of mass spectrometry : JMS》2016,51(8):651-664
The primary functions of adrenal medullary chromaffin cells are the synthesis and storage in their chromaffin vesicles of the catecholamines noradrenaline (NA) and adrenaline (AD), and their subsequent release into the bloodstream by Ca2+‐dependent exocytosis under conditions of fear or stress (fight or flight response). Several monoamines, nucleotides and opiates, such as leucine‐enkephalin (LENK) and methionine‐enkephalin (MENK), are also co‐stored and co‐released with the catecholamines. However, other neurotransmitters have not been studied in depth. Here, we present a novel high‐resolution liquid chromatography‐tandem mass spectrometry approach for the simultaneous monitoring of 14 compounds stored and released in bovine chromaffin cells (BCCs). We validated the analytical method according to the recommendations of the EMA and FDA by testing matrix effect, selectivity, sensitivity, precision, accuracy, stability and carry‐over. After testing on six batches of BCCs from different cultures, the method enabled simultaneous quantitative determination of monoamines (AD, NA, dopamine, serotonin, 5‐hydroxyindoleacetic acid, histamine and metanephrine), amino acids (L‐glutamic acid, γ‐aminobutyric acid), nucleotides (adenosine 5′‐diphosphate, adenosine 5′‐monophosphate, cyclic adenosine 5′‐monophosphate) and neuropeptides (LENK and MENK) in the intracellular content, basal secretion and acetylcholine induced secretion of BBCs. The high‐resolution approach used here enabled us to determine the levels of 14 compounds in the same BCC batch in only 16 min. This novel approach will make it possible to study the regulatory mechanisms of Ca2+ signaling, exocytosis and endocytosis using different neurotrophic factors and/or secretagogues as stimuli in primary BCC cultures. Our method is actually being applied to human plasma samples of different therapeutic areas where sympathoadrenal axis is involved in stress situations such as Alzheimer's disease, migraine or cirrhosis, to improve diagnosis and clinical practice. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
90.
Penno D Lillo V Koshevoy IO Sanaú M Ubeda MA Lahuerta P Fernández E 《Chemistry (Weinheim an der Bergstrasse, Germany)》2008,14(34):10648-10655
Novel Pd(2) (6+) compounds have been synthesized in high yield. These compounds and their Pd(2) (4+) counterparts as synthetic precursors mediate the diboration of vinylarenes and aliphatic 1-alkenes, and under mild and basic reaction conditions they produce a variety of 1,2-diboronate esters with excellent conversions and chemoselectivities. The presence of bis(catecholato)diboron (B(2)cat(2)) favours the reduction of Pd(III) to Pd(II), while the catalytic precursor of Pd(II) is transformed into Pd(0)-nanoparticles. An "in situ" catalytic tandem reaction has been designed to transform the diboronate intermediates into the monoarylated product, which after oxidative workup, provides the carbohydroxylated adduct. Eventually, the same catalyst performs both sequences with total conversion from the alkene. 相似文献