Enantioselective separations by capillary GC on derivatized cyclodextrins. Part 2: Determination of enantiomeric excess of some racemic 2,3-isopropylidene-1,2,3-cyclohexanetriol derivatives on permethyl α-, β-, and γ-cyclodextrins

Capillary GC on permethyl α-, β-, and γ-cyclodextrins has been applied to separate and quantify the enantiomers of some 2,3-iso-propylidene-1,2,3-cyclohexanetriol derivatives. Quantitative CGC data are compared to those obtained with chiral shift ¹H NMR.     

Determination of iphosphamide and seven metabolites in blood plasma,as stable trifluoroacetyl derivatives,by electron capture chemical ionization GC-MS

A method is described for the determination of the antitumor agent iphosphamide and seven of its metabolites in the plasma of cancer patients by multiple ion monitoring (MIM) GC-MS, mainly using the electron capture chemical ionization mode, of stable methyl and/or trifluoroacetyl derivatives. The metabolites determined were 2- and 3-dechloroethyliphosphamide, 4-ketoiphosphamide, carboxyiphosphamide, iphosphamide mustard, and two previously undetected metabolites, chloroethylamine and 1,3-oxazolidine-2-one. The isolation of the acidic and neutral metabolites was performed by solid phase extraction on to C₁₈ adsorbent at pH 4. The weakly acidic iphosphamide mustard, isolated under these conditions with a yield of ca 50%, was measured as a stable methyltrifluoroacetyl derivative, in contrast to the corresponding phosphoramide mustard of the isomer cyclophosphamide which decomposes during derivatization. Chloroethylamine and 1,3-oxazolidine-2-one were isolated with high yield by liquid extraction with ethyl acetate at pH 10. Selective measurement of several metabolite derivatives with similar retention times was performed by multiple ion monitoring MS of specific ion masses, using a methyl phenyl siloxane capillary column previously employed in the study of cyclophosphamide metabolites. Quantitation of metabolites in patient plasma samples could be performed in the concentration range 3 ng to 20 μg per ml of original plasma.     

Trace pollutants analysis in soil by a time-resolved laser-induced breakdown spectroscopy technique

The results of a joint experiment of IFAM-Pisa and ENEA-Frascati for the detection of traces of pollutants in soil by a time-resolved laser-induced spectroscopy technique are reported. Using samples of soil with known pollutants' concentration [Geochemical Exploration Reference (GXR) silicate from US Geological Survey], we were able to estimate the sensitivity of this Laser-Induced Breakdown Spectroscopy (LIBS) technique to be of the order of some parts per million for a vast class of metallic pollutants, including extremely dangerous soil pollutants such as copper, lead and chromium.ENEA guest with HC&M fellowship     

Special geometry,cubic polynomials and homogeneous quaternionic spaces

The existing classification of homogeneous quaternionic spaces is not complete. We study these spaces in the context of certainN=2 supergravity theories, where dimensional reduction induces a mapping betweenspecial real, Kähler and quaternionic spaces. The geometry of the real spaces is encoded in cubic polynomials, those of the Kähler and quaternionic manifolds in homogeneous holomorphic functions of second degree. We classify all cubic polynomials that have an invariance group that acts transitively on the real manifold. The corresponding Kähler and quaternionic manifolds are then homogeneous. We find that they lead to a well-defined subset of the normal quaternionic spaces classified by Alekseevskiî (and the corresponding special Kähler spaces given by Cecotti), but there is a new class of rank-3 spaces of quaternionic dimension larger than 3. We also point out that some of the rank-4 Alekseevskiî spaces were not fully specified and correspond to a finite variety of inequivalent spaces. A simpler version of the equation that underlies the classification of this paper also emerges in the context ofW ₃ algebras.Communicated by K. Gawedzki     

离子束引起的在Al／Cr双层薄膜界面处的原子混合

用600keV的Kr~ 离子轰击Al/Cr双层薄膜样品进行界面原子反应及相互混合的研究。实验样品是在单晶硅上蒸镀约500nm厚的铝膜,相继再蒸上所需厚度的铬膜而制成的。轰击剂量为2.0×10~(15)-2.5×10~(16)Kr~ /cm~2。用2.0MeVa粒子对轰击前后的样品进行了卢瑟福背散射(RBS)分析,发现界面处有明显的原子混合存在;当轰击剂量≥1.0×10~(16)Kr~ /cm~2时,RBS谱出现有明显的坪台,经拟合计算和x射线衍射(XRD)测量证实确有化合物Al_(13)Cr_2存在;还分别得到了原子混合量及混合效率与轰击剂量的关系;最后对界面处的原子混合机制进行了讨论。     

Mössbauer spectroscopic study of core and mantle of rattle stones

Mössbauer spectroscopy has been applied to two rattle stones found in the Netherlands, which have been formed under different pedoenvironment conditions in the past. Ultrafine particles of goethite are dominantly present in both core and mantle of these stones. The latter differs in both composition and mean particle size; this is related to the distinct manner of their formation.     

Consequences of an algorithm for bridged graphs

Chepoi showed that every breadth first search of a bridged graph produces a cop-win ordering of the graph. We note here that Chepoi's proof gives a simple proof of the theorem that G is bridged if and only if G is cop-win and has no induced cycle of length four or five, and that this characterization together with Chepoi's proof reduces the time complexity of bridged graph recognition. Specifically, we show that bridged graph recognition is equivalent to (C₄,C₅)-free graph recognition, and reduce the best known time complexity from O(n⁴) to O(n^3.376).     

一个在水稻胚胎中差异表达基因的分离鉴定

通过筛选水稻开花后48～50h的原胚和120～122h的分化胚的cDNA文库,得到一个在胚胎发育过程中表达有差异的克隆,命名为OsEES,测序结果显示OsEES含有一个预测的亚精氨合成酶结构域,编码一个大小约38×103(pI6.5)的蛋白.同时RNA原位杂交的结果显示开花48～50h的原胚中的信号强度明显大于开花120～122h的分化胚,表明OsEES可能在水稻胚胎发生过程中起到一定的调控作用.     

基于cDNA宏阵列的系统聚类分析猪发育阶段的基因表达谱

取杜洛克猪胚胎第33,45,55，65．75天的背最长肌样本．用cDNA Macroarray分析方法和聚类分析技术分析了327个EST在骨骼肌内不同发育阶段的基因表达谱．结果表明有98条EST在不同发育时期显著差异表达．第33天和第45天两阶段基因表达状态相似．第55天和第65天基因表达状态相似．而第75天的基因表达与第55天和第65天两个阶段的基因表达具有相近的聚类关系．表达状态相近，基因功能相似的基因大都被聚类在一起．