Conjugation of linoleic acid over a hydrogen pre-activated heterogeneous catalyst

Development of a new heterogeneous catalytic pathway for isomerization of linoleic acid to conjugated linoleic acids at mild reaction conditions over Ni/H-MCM-41 in a soluted system.     

Thapsigargin,constitution of a sesquiterpene lactone histamine liberator from thapsia garganica

The major skin irritating constituent of the root of Thapsia garganica L. has been shown to be a hexaoxygenated-C₆-guaianolide esterificated with four carboxylic acids.     

Comparison of chromatographic ion-exchange resins. III. Strong cation-exchange resins

A comparative study was performed on strong cation-exchangers to investigate the pH dependence, efficiency, binding strength, particle size distribution, static and dynamic capacity, and SEM pictures of chromatographic resins. The resins tested included: SP Sepharose XL, Poros 50 HS, Toyopearl SP 550c, SP Sepharose BB, Source 30S, TSKGel SP-5PW-HR20, and Toyopearl SP 650c. Testing was performed with four different proteins: anti-FVII Mab (IgG), aprotinin, lysozyme, and myoglobin. Dependence of pH on retention was generally very low for proteins with high pI. An unexpected binding at pH 7.5 of anti-FVII Mab with pI < 7.5 was observed on several resins. Efficiency results show the expected trend of higher dependence of the plate height with increasing flow rate of soft resins compared to resins for medium and high-pressure operation. Determination of particle size distribution by two independent methods, Coulter counting and SEM, was in very good agreement. The mono-dispersed nature of Source 30S was confirmed. Binding to cation-exchange resins as a function of ionic strength varies depending on the specific protein. Generally, binding and elution at high salt concentration may be performed with Toyopearl SP 550c and Poros 50 HS, while binding and elution at low salt concentration may be performed with Toyopearl SP 650c. A very high binding capacity was obtained with SP Sepharose XL. Comparison of static capacity and dynamic capacity at 10% break-through shows in general approximately 50-80% utilisation of the total available capacity during chromatographic operation. A general good agreement was obtained between this study and data obtained by others. The results of this study may be used for selection of resins for testing in process development. The validity of experiments and results with model proteins were tested using human insulin precursor in pure state and in real feed-stock on Toyopearl SP 550c, SP Sepharose BB, and Toyopearl SP 650c. Results showed good agreement with experiments with model proteins.     

Identification of the species formed in the Fe/MgO system: A Raman and Mössbauer study

A set of samples of Fe/MgO, in the concentration range between 1.5 and 9 wt% of Fe, was prepared by the impregnation method and then calcined at 1073 K. All of them were characterized by XRD and Raman and Mössbauer spectroscopies. The data obtained show that the solids are made up of MgFeO₄ nanoclusters and Fe³⁺ ions dispersed in the MgO matrix. The size of the clusters and the amounts of each phase were calculated using the XRD and Mössbauer data.     

Some transformation techniques with applications in global optimization

In this paper some transformation techniques, based on power transformations, are discussed. The techniques can be applied to solve optimization problems including signomial functions to global optimality. Signomial terms can always be convexified and underestimated using power transformations on the individual variables in the terms. However, often not all variables need to be transformed. A method for minimizing the number of original variables involved in the transformations is, therefore, presented. In order to illustrate how the given method can be integrated into the transformation framework, some mixed integer optimization problems including signomial functions are finally solved to global optimality using the given techniques.     

Measurement and modeling of diffraction from an edge of a thin panel

The diffraction of sound from an edge of a thin chipboard panel was measured in an anechoic chamber, and compared to simulations based on the diffraction formulation developed by Svensson et al. [Svensson UP, Fred RI, Vanderkooy J. An analytic secondary source model of edge diffraction impulse responses. J Acoust Soc Am 1999;106(5):2331-44]. The measurements and simulations were performed for a line of receiver positions below the panel to include cases for which the direct sound had an unobstructed propagation path to the receivers, as well as cases for which the direct sound was occluded by the panel. Comparison of the measured and simulated responses is provided in both the time and frequency-domains, and shows that the differences between them are small over the entire audible frequency range. This case study verifies that the applied diffraction-modeling method gives accurate results, and that the assumptions of ideal source and wedge characteristics inherent in the method do not preclude its use in simulations of realistic scenarios.     

Antibacterial activity of N-quaternary chitosan derivatives: Synthesis, characterization and structure activity relationship (SAR) investigations

Quaternary N-(2-(N,N,N-tri-alkyl ammoniumyl and 2-pyridiniumyl) acetyl) derivatives of chitosan polymer, chitooligomer, and glucosamine (monomer) were synthesized for the purpose of investigating the structure activity relationship (SAR) for the antibacterial effect. Novel methods were used in the synthesis. The final chitosan and chitooligomer derivatives could thus be obtained in two steps without prior protection of the hydroxyl groups. However, in order to obtain chitosan derivatives with the bulky N,N-dimethyl-N-dodecyl- and N,N-dimethyl-N-butyl side chains three steps were needed, starting from 3,6-O-di-tert-butyldimethylsilyl chitosan (3,6-O-di-TBDMS chitosan) as the key intermediate. The quaternary ammoniumyl acetyl derivatives of glucosamine were synthesized from glucosamine or tetra-O-acetylglucosamine. N,N,N-trimethyl chitosan (TMC) was used as reference compound for investigation of antibacterial activity. Clinical Laboratory Standard Institute (CLSI) protocols were used to determine MIC and MLC for activity against clinically important Gram-positive strains Staphylococcus aureus (ATCC 25923), and S. aureus (MRSA) (ATCC 43300), and Gram-negative strains of Escherichia coli (ATCC 25922), P. aeriginosa (ATCC 27853) and Enterococcus facialis (ATCC 29212). The MIC values for the compounds ranged from 8 to ?8192 mg/L. In general the N-(2-(N,N-dimethyl-N-dodecyl ammoniumyl) acetyl) derivatives of chitooligomer and glucosamine monomer were more active against bacteria than derivatives with shorter alkyl chains. In contrast the N-(2-(N,N-dimethyl-N-dodecyl ammoniumyl) acetyl) derivatives of chitosan were less active than derivatives with N-(2-N,N,N-trimetylammoniumyl) acetyl or N-(2-(N-pyridiniumyl) acetyl) quaternary moiety. N,N,N-trimethyl chitosan (TMC) was the most active compound in this study.     

Lateral reflections are favorable in concert halls due to binaural loudness

A recent study on perceptual difference in simulated concert halls showed that a concert hall renders stronger sound with more bass when the temporal envelope of a signal is preserved in the reflections [Lokki et al., J. Acoust. Soc. Am. 129, EL223-EL228 (2011)]. In the same study the lateral reflections were shown to contribute to the perceived envelopment and openness. Moreover, the listening test results suggest that lateral reflections contribute to perception of sound source distance. Here, it is shown that lateral reflections are beneficial due to their increasing effect on binaural loudness-the phenomenon known well in psychoacoustics, but not in architectural acoustics. The reflections from the side are amplified more than median plane reflections, in particular at high frequencies, due to the shape of the human head.     

Investigations on the balloon as an impulse source

Measurements of impulses produced by bursting balloons are presented. Various sizes of balloons were popped with a mechanical device in an anechoic chamber and recorded with a spherical microphone array. The power responses and directivity of the balloons are analyzed. Results indicate that power responses have two emphasized frequencies which depend on balloon size and inflation level. Larger balloons radiated more energy and higher inflation levels resulted in stronger high frequency content. Balloon directivity patterns are stable over repetitions. However, balloons do not radiate omnidirectionally. The degree of omnidirectionality improves with balloon size and for midrange frequencies.