Recognition of Dimethylarginine Analogues by Tandem Tudor Domain Protein Spindlin1

Epigenetic readout of the combinatorial posttranslational modification comprised of trimethyllysine and asymmetric dimethylarginine (H3K4me3R8me2a) takes place via biomolecular recognition of tandem Tudor-domain-containing protein Spindlin1. Through comparative thermodynamic data and molecular dynamics simulations, we sought to explore the binding scope of asymmetric dimethylarginine mimics by Spindlin1. Herein, we provide evidence that the biomolecular recognition of H3K4me2R8me2a is not significantly affected when R8me2a is replaced by dimethylarginine analogues, implying that the binding of K4me3 provides the major binding contribution. High-energy water molecules inside both aromatic cages of the ligand binding sites contribute to the reader–histone association upon displacement by histone peptide, with the K4me3 hydration site being lower in free energy due to a flip of Trp151.     

FUNDAMENTALS OF TRANSMISSION FLUCTUATION SPECTROMETRY WITH VARIABLE SPATIAL AVERAGING

Transmission signal of radiation in suspension of particles performed with a high spatial and temporal resolution shows significant fluctuations,which are related to the physical properties of the particles and the process of spatial and temporal averaging.Exploiting this connection,it is possible to calculate the particle size distribution (PSD) and particle concentration.This paper provides an approach of transmission fluctuation spectrometry (TFS) with variable spatial averaging,The transmission fluctuations are expressed in terms of the expectancy of transmission square (ETS) and are obtained as a spectrum,which is a function of the variable beam diameter.The reversal point and the depth of the spectrum contain the information of particle size and particle concentration,respectively.     

XNBR/LDH nanocomposites: Effect of vulcanization and organic modifier on nanofiller dispersion and strain‐induced crystallization

In elastomer/organo clay nanocomposites, the morphological characteristics, and hence the mechanical properties, of the vulcanizates are strongly influenced by the organic modifier and the vulcanization process. When the elastomer itself undergoes strain‐induced crystallization, both the organic modifier and the dispersed filler particles could significantly influence the crystallization process. These phenomena are very common in case of natural rubber‐based vulcanizates. In this study, the similar effects have been demonstrated with carboxylated nitrile rubber (XNBR) and organically modified layered double hydroxide (O‐LDH)‐based nanocomposites. The effect of size of the organic modifier was obviously visible on the interlayer distance of O‐LDH and also on the morphological reorganization of the dispersed O‐LDH particles during vulcanization process. The strain‐induced crystallization of the XNBR was found to be strongly dependent on the morphological change that occurs during vulcanization process. © 2010 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2010     

Lie geometry of flat fronts in hyperbolic space

We propose a Lie geometric point of view on flat fronts in hyperbolic space as special Ω-surfaces and discuss the Lie geometric deformation of flat fronts.