New measurement of the neutron lifetime with a large gravitational trap

The lifetime of the neutron is one of the key physical quantities used to determine the weak interaction parameters and to test predictions of the theory of primary nucleosynthesis. The lifetime of the neutron has been measured in the reported experiment by the method of storing neutrons in a material trap with a gravitational valve. Fomblin grease UT-18 hydrogen-free fluorine polymer has been used as coating. The resistance of the coating to repeated cooling down to 80 K combined with heating up to 300 K has been studied. The probability of losses in the trap is as small as 1.5% of the neutron decay probability. The lifetime of the neutron τ_n = (881.5 ± 0.7_stat ± 0.6_syst)s obtained at the new step is in good agreement with a commonly accepted value of (880.2 ± 1.0) s presented by the Particle Data Group.     

The effect of point mutation on the equilibrium structural fluctuations of ferric Myoglobin

The ultrafast equilibrium fluctuations of the Fe(III)-NO complex of a single point mutation of Myoglobin (H64Q) have been studied using Fourier Transform 2D-IR spectroscopy. Comparison with data from wild type Myoglobin (wt-Mb) shows the presence of two conformational substates of the mutant haem pocket where only one exists in the wild type form. One of the substates of the mutant exhibits an almost identical NO stretching frequency and spectral diffusion dynamics to wt-Mb while the other is distinctly different in both respects. The remarkably contrasting dynamics are largely attributable to interactions between the NO ligand and a nearby distal side chain which provides a basis for understanding the roles of these side chains in other ferric haem proteins.     

Unraveling the mechanism of catalytic reduction of O2 by microperoxidase-11 adsorbed within a transparent 3D-nanoporous ITO film

Nanoporous films of indium tin oxide (ITO), with thicknesses ranging from 250 nm to 2 μm, were prepared by Glancing Angle Deposition (GLAD) and used as highly sensitive transparent 3D-electrodes for quantitatively interrogating, by time-resolved spectroelectrochemistry, the reactivity of microperoxidase-11 (MP-11) adsorbed within such films. The capacitive current densities of these 3D-electrodes as well as the amount of adsorbed MP-11 were shown to be linearly correlated to the GLAD ITO film thickness, indicating a homogeneous distribution of MP-11 across the film as well as homogeneous film porosity. Under saturating adsorption conditions, MP-11 film concentration as high as 60 mM was reached. This is equivalent to a stack of 110 monolayers of MP-11 per micrometer film thickness. This high MP-11 film loading combined with the excellent ITO film conductivity has allowed the simultaneous characterization of the heterogeneous one-electron transfer dynamics of the MP-11 Fe(III)/Fe(II) redox couple by cyclic voltammetry and cyclic voltabsorptometry, up to a scan rate of few volts per second with a satisfactory single-scan signal-to-noise ratio. The potency of the method to unravel complex redox coupled chemical reactions was also demonstrated with the catalytic reduction of oxygen by MP-11. In the presence of O(2), cross-correlation of electrochemical and spectroscopic data has allowed us to determine the key kinetics and thermodynamics parameters of the redox catalysis that otherwise could not be easily extracted using conventional protein film voltammetry. On the basis of numerical simulations of cyclic voltammograms and voltabsorptograms and within the framework of different plausible catalytic reaction schemes including appropriate approximations, it was shown possible to discriminate between different possible catalytic pathways and to identify the relevant catalytic cycle. In addition, from the best fits of simulations to the experimental voltammograms and voltabsorptograms, the partition coefficient of O(2) for the ITO film as well as the values of two kinetic rate constants could be extracted. It was finally concluded that the catalytic reduction of O(2) by MP-11 adsorbed within nanoporous ITO films occurs via a 2-electron mechanism with the formation of an intermediate Fe(III)-OOH adduct characterized by a decay rate of 11 s(-1). The spectroelectroanalytical strategy presented here opens new opportunities for characterizing complex redox-coupled chemical reactions not only with redox proteins, but also with redox biomimetic systems and catalysts. It might also be of great interest for the development and optimization of new spectroelectrochemical sensors and biosensors, or eventually new photoelectrocatalytic systems or biofuel cells.     

LES of heat transfer in electronics

Numerical methods based on the Reynolds Averaged Navier–Stokes (RANS) and Large Eddy Simulation (LES) equations are applied to the thermal prediction of flows representative of those found in and around electronics systems and components. Low Reynolds number flows through a heated ribbed channel, around a heated cube and within a complex electronics system case are investigated using linear and nonlinear LES models, hybrid RANS–LES and RANS–Numerical-LES (RANS–NLES) methods. Flow and heat transfer predictions using these techniques are in good agreement with each other and experimental data for a range of grid resolutions. Using second order central differences, the RANS–NLES method performs well for all simulations.     

A high concentration of genistein down-regulates activin A, Smad3 and other TGF-β pathway genes in human uterine leiomyoma cells

Previously, we found that high doses of genistein show an inhibitory effect on uterine leiomyoma (UtLM) cell proliferation. In this study, using microarray analysis and Ingenuity Pathways Analysis™, we identified genes (up- or down-regulated, ≥ 1.5 fold, P ≤ 0.001), functions and signaling pathways that were altered following treatment with an inhibitory concentration of genistein (50 µg/ml) in UtLM cells. Downregulation of TGF-β signaling pathway genes, activin A, activin B, Smad3, TGF-β2 and genes related to cell cycle regulation, with the exception of the upregulation of the CDK inhibitor P15, were identified and validated by real-time RT-PCR studies. Western blot analysis further demonstrated decreased protein expression of activin A and Smad3 in genistein-treated UtLM cells. Moreover, we found that activin A stimulated the growth of UtLM cells, and the inhibitory effect of genistein was partially abrogated in the presence of activin A. Overexpression of activin A and Smad3 were found in tissue samples of leiomyoma compared to matched myometrium, supporting the contribution of activin A and Smad3 in promoting the growth of UtLM cells. Taken together, these results suggest that down-regulation of activin A and Smad3, both members of the TGF-β pathway, may offer a mechanistic explanation for the inhibitory effect of a high-dose of genistein on UtLM cells, and might be potential therapeutic targets for treatment of clinical cases of uterine leiomyomas.     

Conservation laws and stress–energy–momentum tensors for systems with background fields

This article attempts to delineate the roles played by non-dynamical background structures and Killing symmetries in the construction of stress–energy–momentum tensors generated from a diffeomorphism invariant action density. An intrinsic coordinate independent approach puts into perspective a number of spurious arguments that have historically lead to the main contenders, viz the Belinfante–Rosenfeld stress–energy–momentum tensor derived from a Noether current and the Einstein–Hilbert stress–energy–momentum tensor derived in the context of Einstein’s theory of general relativity. Emphasis is placed on the role played by non-dynamical background (phenomenological) structures that discriminate between properties of these tensors particularly in the context of electrodynamics in media. These tensors are used to construct conservation laws in the presence of Killing Lie-symmetric background fields.     

Large-eddy simulations of chevron jet flows with noise predictions

Hybrid large-eddy type simulations for chevron nozzle jet flows are performed at Mach 0.9 and Re = 1.03 × 10⁶. Without using any subgrid scale model (SGS), the numerical approach applied in the present study is essentially implicit large-eddy simulation (ILES). However, a Reynolds-averaged Navier–Stokes (RANS) solution is patched into the near wall region. This makes the overall solution strategy hybrid RANS–ILES. The disparate turbulence length scales, implied by these different modeling approaches, are matched using a Hamilton–Jacobi equation. The complex geometry features of the chevron nozzles are fully meshed. With numerical fidelity in mind, high quality, hexahedral multi-block meshes of 12.5 × 10⁶ cells are used. Despite the modest meshes, the novel RANS–ILES approach shows encouraging performance. Computed mean and second-order fluctuating quantities of the turbulent near field compare favorably with measurements. The radiated far-field sound is predicted using the Ffowcs Williams and Hawkings (FW–H) surface integral method. Encouraging agreement of the predicted far-field sound directivity and spectra with measurements is obtained.     

An anisotropic rotary diffusion model for fiber orientation in short- and long-fiber thermoplastics

The Folgar–Tucker model, which is widely-used to predict fiber orientation in injection-molded composites, accounts for fiber–fiber interactions using isotropic rotary diffusion. However, this model does not match all aspects of experimental fiber orientation data, especially for composites with long discontinuous fibers. This paper develops a fiber orientation model that incorporates anisotropic rotary diffusion. From kinetic theory we derive the evolution equation for the second-order orientation tensor, correcting some errors in earlier treatments. The diffusivity is assumed to depend on a second-order space tensor, which is taken to be a function of the orientation state and the rate of deformation. Model parameters are selected by matching the experimental steady-state orientation in simple shear flow, and by requiring stable steady states and physically realizable solutions. Also, concentrated fiber suspensions align more slowly with respect to strain than models based on Jeffery's equation, and we incorporate this behavior in an objective way. The final model is suitable for use in mold filling and other flow simulations, and it gives improved predictions of fiber orientation for injection molded long-fiber composites.     

Continuous countercurrent extraction of hemicellulose from pretreated wood residues

Two-stage dilute acid pretreatment followed by enzymatic cellulose hydrolysisis an effectivemethod for obtaining high sugar yields from wood residuessuchassoftwood forest thinnings. In the first-stage hydrolysis step, most of the hemicellulose is solubilized using relatively mild conditions. The soluble hemicellu losic sugars are recovered from the hydrolysateslurry by washing with water. The washed solids are then subjected tomoresevere hydrolysis conditions to hydrolyze approx 50% of the cellulose to glucose. The remaining cellulose can further be hydrolyzed with cellulase enzyme. Our process simulation indicates that the amount of water used in the hemicellulose recovery step has a significan tim pact on the cost of ethanol production. It is important to keep water usage as low as possible while mainta ining relatively high recovery of solublesugars. To achieve this objective, a prototype pilot-scale continuous countercurrent screw extractor was evaluated for the recovery of hemicellulose from pretreated forest thinnings. Using the 274-cm (9-ft) long extractor, solubles recoveries of 98, 91, and 77% were obtained with liquid-to-insoluble solids (L/1S) ratios of 5.6, 3.4, and 2.1, respectively. An empirical equation was developed to predict the performance of the screwextractor. This equation predicts that soluble sugar recovery above 95% can be obtained with an L/IS ratio as low as 3.0.     

Fourier transform infrared quantification of sugars in pretreated biomass liquors

The process of converting renewable lignocellulosic biomass to ethanol requires a number of steps, and pretreatment is one of the most important. Pretreatment usually in volves a hydrolysis of the easily hydrolyzed hemicellulosic component of biomass using some form of thermal/chemical/mechanical action that results in a product that can be further hydrolyzed by cellulase enzymes (the cellulosic portion). The sugars produced can then befermented to ethanol by fermentative microorganisms. If the pretreatment step is not severe enough, the resultant residue is not as easily hydrolyzed by the cellulase enzyme. More severe pretreatment conditions result in the production of degradation products that are toxic to the fermentative microorgan ism. In this article, wereport the quantitative analysis of glucose, mannose, xylose, and acetic acid using Fourier transform infrared (FTIR) spectroscopy on liquors from dilute-acid-pretreated softwood and hard wood slurries. Comparison of FTIR and high-performance liquid chromatography quantitative analyses of these liquorsare reported. Recent developments in infrared probe technology has enabled the rapid quantification of these sugars by FTIR spectroscopy in the batch reactor during optimization of the pretreatment conditions, or interfaced to the computer controlling a continuous reactor for on-line monitoring and control.