Biosensor chip mass spectrometry: a chip-based proteomics approach

Rapid advances in genomic sequencing, bioinformatics, and analytical instrumentation have created the field of proteomics, which at present is based largely on two-dimensional electrophoresis (2-DE) separation of complex protein mixtures and identification of individual proteins using mass spectrometry. These analyses provide a wealth of data, which upon further evaluation leads to many questions regarding the structure and function of the proteins. The challenge of answering these questions create a need for high-specificity approaches that may be used in the analysis of biomolecular recognition events and interacting partners, and thereby places great demands on general protein characterization instrumentation and the types of analyses they need to perform. Over the past five years we have been actively involved in interfacing two general, instrumental techniques, surface plasmon resonance-biomolecular interaction analysis (SPR-BIA) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, into a single concerted approach for use in the functional and structural characterization of proteins. Reviewed here is the recent progress made using biomolecular interaction analysis - mass spectrometry (BIA-MS) in the detailed characterization of proteins and protein-protein interactions and the development of biosensor chip mass spectrometry (BCMS) as a new chip-based proteomics approach.     

Synthesis and characterization of mononuclear zinc aryloxide complexes supported by nitrogen/sulfur ligands possessing an internal hydrogen bond donor

Treatment of a dinuclear zinc hydroxide complex ([(bmnpaZn)(2)(mu-OH)(2)](ClO(4))(2) (1) or [(benpaZn)(2)(mu-OH)(2)](ClO(4))(2) (2)) with excess equivalents of an aryl alcohol derivative (p-HOC(6)H(4)X; X = NO(2), CHO, CN, COCH(3), Br, H, OCH(3)) yielded the nitrogen/sulfur-ligated zinc aryloxide complexes [(bmnpa)Zn(p-OC(6)H(4)NO(2))](ClO(4)) (3), [(benpa)Zn(p-OC(6)H(4)NO(2))](ClO(4)) (4), [(benpa)Zn(p-OC(6)H(4)CHO)](ClO(4)) (5), [(benpa)Zn(p-OC(6)H(4)CN)](ClO(4)) (6), [(benpa)Zn(p-OC(6)H(4)COCH(3))](ClO(4)) x 0.5H(2)O (7), [(benpa)Zn(p-OC(6)H(4)Br)](ClO(4)) (8), [(benpa)Zn(p-OC(6)H(5))](ClO(4)) (9), and [(benpa)Zn(p-OC(6)H(5)OCH(3))](ClO(4)) (10). The solid state structures of 2, 3, 5, and 6 have been determined by X-ray crystallography. While 3 and 6 exhibit a mononuclear zinc ion possessing a distorted five-coordinate trigonal bipyramidal geometry, in 5 each zinc center exhibits a distorted six-coordinate octahedral geometry resulting from coordination of the aldehyde carbonyl oxygen of another zinc-bound aryloxide ligand, yielding a chain-type structure. Zinc coordination of the aldehyde carbonyl of 5 is indicated by a large shift (>40 cm(-)(1)) to lower energy of the carbonyl stretching vibration (nu(C[double bond]O) in solid state FTIR spectra of the complex. In the solid state structures of 3, 5, and 6, a hydrogen-bonding interaction is found between N(3)-H of the supporting bmnpa/benpa ligand and the zinc-bound oxygen atom of the aryloxide ligand (N(3)...O(1) approximately 2.78 A). Solution (1)H and (13)C NMR spectra of 3-10 in CD(3)CN and FTIR spectra in CH(3)CN are consistent with all of the aryloxide complexes having a similar solution structure, with retention of the hydrogen-bonding interaction involving N(3)-H and the oxygen atom of the zinc-coordinated aryloxide ligand. For this family of zinc aryloxide complexes, a correlation was discovered between the chemical shift position of the N(3)-H proton resonance and the pK(a) of the parent aryl alcohol. This correlation indicates that the strength of the hydrogen-bonding interaction involving the zinc-bound aryloxide oxygen is increasing as the aryloxide moiety increases in basicity.     

Analytic subgroups of -modules

In this paper we study the structure of analytic subgroups and of -submodules of -modules.

     

医药类专业物理课程教学存在的问题及对策

根据对国内69所普通高等院校医药类专业（本科）物理课程教学现状调查的结果,分析了在我国高等教育进入“大众化教育”的形势下,医药类专业物理课程教学中存在的4个急需解决的问题,指出了医药类专业物理课程教学改革的方向,并介绍了我们所做的一些工作。     

Spontaneous lepton-number violation and de-leptonization in stellar collapse

We consider core collapse in weak interaction models where global lepton-number invariance is spontaneously broken. We find that the initial neutrino number prevents finite temperature restoration of lepton-number conservation. Assuming the lepton-number violating reactions occur rapidly, we show that the neutrino number is driven to a sufficiently small value to allow electron capture reactions to de-leptonize the core, and lead to a high-entropy collapse.     

Heavy-ion fusion: Comparison of experimental data with classical trajectory models

Currently available data on fusion excitation functions for heavy-ion induced reactions over a wide mass range are compared to results calculated with a classical dynamical model based on the proximity nuclear potential of Blocki et al., the Coulomb potential of Bondorf et al., and one-body nuclear friction in the proximity formalism of Randrup. With these conservative and dissipative forces and the radial parameters of Myers, overall good agreement is obtained between the theoretical excitation functions and most of the available data. Extensive calculations have been performed to test the sensitivity of the calculated fusion cross-sections to a number of parameters, including the radial dependence of the Coulomb and nuclear potentials, the radial and tangential friction form factors as well as the projectile and target radii. The theoretical excitation functions for the lighter heavy-ion systems are rather insensitive to changes in either the conservative or dissipative forces. The calculations show that tangential friction sufficient to produce the rolling condition is necessary to explain the magnitude of the fusion cross-sections at high energies, which are also sensitive to the magnitude of the radial friction component. This is in contrast to the fusion cross-sections at low energies which are determined by the nuclear potential at larger separations, and to a lesser extent by tangential friction. The low energy fusion data are most sensitive to the nuclear radii. The calculations reveal the importance of more experimental measurements of fusion cross-sections at high energies, especially for heavy systems where the magnitudes of the fusion cross-sections are the most sensitive to the assumed forces. However, even for these cases the effects of the conservative and dissipative forces are similar and difficult to separate. These studies indicate, however, that it is possible to construct a conservative potential that will give calculated fusion excitation functions which are in good agreement with all experimental data over the entire mass range. The maximum fusion cross-sections as defined here exceed considerably the liquid-drop limiting value for heavy systems.